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Bep neon transfection system

Manufactured by Thermo Fisher Scientific
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The BEP (Neon Transfection System) is a laboratory instrument designed for the efficient delivery of nucleic acids, such as DNA or RNA, into a variety of cell types. It utilizes an electroporation-based method to temporarily permeabilize the cell membrane, allowing the introduction of genetic material into the cells.

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Lab products found in correlation

Gene gun pds 1000 he system, by Bio-Rad (2 mentions) Lipofectamine 2000, by Bio-Rad (2 mentions)

Close spelling variants of this product

Neon Transfection System Neon transfection Neon system NeonTM

2 protocols using bep neon transfection system

1

Optimized Cellular Transfection via Nanochannels

Check if the same lab product or an alternative is used in the 5 most similar protocols
For CNP, a single layer of MEFs, MSCs, DCs, or HEK- 293T cells (~200,000 cells) was spread on a 1 cm x 1 cm 3D CNP silicon chip surface for overnight cell incubation. Plasmids pre-loaded in PBS buffer were injected into individual cells via nanochannels (~500 nm diameter and 5 μm spacing) using a 200 V electric field for 5 pulses at 10 ms/pulse with a 0.1 second interval. Various electroporation conditions were tested for best choice. BEP (Neon Transfection System, Thermo Fisher Scientific), Gene Gun (PDS-1000/He™ System, Bio-Rad) and Lipofectamine 2000 transfections were conducted according to manufacturers’ instructions. Ascl1/Brn2/Myt1l plasmids at a weight ratio of 2/1/1 and a concentration of 100 ng/mL in PBS buffer, according to the protocol in literature,40 were pre-mixed for transfection. Cell transfection of PTEN, miR-128, CD47, CDX-CD47, CREKA-CD47 and CD63-GFP plasmids followed the same procedure.
Yang Z., Shi J., Xie J., Wang Y., Sun J., Liu T., Zhao Y., Zhao X., Wang X., Ma Y., Malkoc V., Chiang C., Deng W., Chen Y., Fu Y., Kwak K.J., Fan Y., Kang C., Yin C., Rhee J., Bertani P., Otero J., Lu W., Yun K., Lee A.S., Jiang W., Teng L., Kim B.Y, & Lee L.J. (2019). Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nature biomedical engineering, 4(1), 69-83.
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2

Optimized Cellular Transfection via Nanochannels

Check if the same lab product or an alternative is used in the 5 most similar protocols
For CNP, a single layer of MEFs, MSCs, DCs, or HEK- 293T cells (~200,000 cells) was spread on a 1 cm x 1 cm 3D CNP silicon chip surface for overnight cell incubation. Plasmids pre-loaded in PBS buffer were injected into individual cells via nanochannels (~500 nm diameter and 5 μm spacing) using a 200 V electric field for 5 pulses at 10 ms/pulse with a 0.1 second interval. Various electroporation conditions were tested for best choice. BEP (Neon Transfection System, Thermo Fisher Scientific), Gene Gun (PDS-1000/He™ System, Bio-Rad) and Lipofectamine 2000 transfections were conducted according to manufacturers’ instructions. Ascl1/Brn2/Myt1l plasmids at a weight ratio of 2/1/1 and a concentration of 100 ng/mL in PBS buffer, according to the protocol in literature,40 were pre-mixed for transfection. Cell transfection of PTEN, miR-128, CD47, CDX-CD47, CREKA-CD47 and CD63-GFP plasmids followed the same procedure.
Yang Z., Shi J., Xie J., Wang Y., Sun J., Liu T., Zhao Y., Zhao X., Wang X., Ma Y., Malkoc V., Chiang C., Deng W., Chen Y., Fu Y., Kwak K.J., Fan Y., Kang C., Yin C., Rhee J., Bertani P., Otero J., Lu W., Yun K., Lee A.S., Jiang W., Teng L., Kim B.Y, & Lee L.J. (2019). Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nature biomedical engineering, 4(1), 69-83.
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