Mononuclear cells (1×106/100 μl) from peripheral blood or bone marrow of AML patients were stained with the following antibodies (all from BD unless stated otherwise, dilution used and catalogue number in parentheses): anti-CD45RA-FITC (1:25, 555488), anti-CD90-APC (1:50, 561971), anti-CD135-Biotin (1:10, 624008), anti-CD38-PE-Cy7 (1:200, 335790), anti-CD10-Alexa-700 (1:10, 624040), anti-CD7-Pacific Blue (1:50, 642916), anti-CD45-V500 (1:200, 560777), anti-CD34-APC-Cy7 (1:100, custom made by BD, CD34 clone 581), anti-CD34-PerCP-Efluor 710 (1:100, e-Bioscience 46-0344-42), anti-CD33-PE-Cy5 (1:100, Beckman Coulter PNIM2647U), anti-CD19-PE (1:200, 349204), anti-CD3-FITC (1:100, 349201), anti-CD56-Alexafluor 647 (1:100, 557711), and Streptavidin-QD605 (1:200, Invitrogen Q10101MP). Samples from Patients #1, 10, 11 (remission sample only), 32, 35, and 55 were enriched for CD34+ cells using a Miltenyi CD34 MicroBead kit according to the manufacturer’s protocol prior to antibody staining. Cells were sorted on a FACS AriaIII to a post-sort purity of >95%.
Anti cd135 biotin
Manufactured by BD
Anti-CD135-Biotin is a laboratory reagent used for the detection and identification of CD135-positive cells in biological samples. It is a biotinylated monoclonal antibody that specifically binds to the CD135 antigen, also known as Fms-like tyrosine kinase 3 (FLT3). The biotinylation of the antibody allows for further detection and quantification of the target cells using appropriate detection methods.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 fitc, by Thermo Fisher Scientific (2 mentions)
Anti cd45 v500, by BD (2 mentions)
Anti cd38 pe cy7, by BD (2 mentions)
Anti cd45ra fitc, by BD (2 mentions)
Cd34 microbead kit, by Miltenyi Biotec (2 mentions)
Anti cd34 percp efluor 710, by Thermo Fisher Scientific (2 mentions)
Anti cd19 pe, by Thermo Fisher Scientific (2 mentions)
Anti cd90 apc, by BD (2 mentions)
Anti cd33 pe cy5, by Beckman Coulter (2 mentions)
Anti cd10 alexa 700, by BD (2 mentions)
Anti cd7 pacific blue, by BD (2 mentions)
Anti cd34 apc cy7, by BD (2 mentions)
Anti cd56 alexafluor 647, by Thermo Fisher Scientific (2 mentions)
Streptavidin qd605, by Thermo Fisher Scientific (2 mentions)
2 protocols using anti cd135 biotin
1
AML Immune Profiling Protocol
2
AML Immune Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mononuclear cells (1×106/100 μl) from peripheral blood or bone marrow of AML patients were stained with the following antibodies (all from BD unless stated otherwise, dilution used and catalogue number in parentheses): anti-CD45RA-FITC (1:25, 555488), anti-CD90-APC (1:50, 561971), anti-CD135-Biotin (1:10, 624008), anti-CD38-PE-Cy7 (1:200, 335790), anti-CD10-Alexa-700 (1:10, 624040), anti-CD7-Pacific Blue (1:50, 642916), anti-CD45-V500 (1:200, 560777), anti-CD34-APC-Cy7 (1:100, custom made by BD, CD34 clone 581), anti-CD34-PerCP-Efluor 710 (1:100, e-Bioscience 46-0344-42), anti-CD33-PE-Cy5 (1:100, Beckman Coulter PNIM2647U), anti-CD19-PE (1:200, 349204), anti-CD3-FITC (1:100, 349201), anti-CD56-Alexafluor 647 (1:100, 557711), and Streptavidin-QD605 (1:200, Invitrogen Q10101MP). Samples from Patients #1, 10, 11 (remission sample only), 32, 35, and 55 were enriched for CD34+ cells using a Miltenyi CD34 MicroBead kit according to the manufacturer’s protocol prior to antibody staining. Cells were sorted on a FACS AriaIII to a post-sort purity of >95%.
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