Camk2n1 human cdna clone

Human prostate cancer LNCaP cells were obtained from ATCC and maintained in in RPMI 1640 medium (Invitrogen) supplemented with 10% FBS or 10% charcoal/dextran stripped FBS (cFBS). C4-2 was maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% FBS. The CAMK2N1 human cDNA clone was purchased from OriGene Technologies and subcloned into the EcoRI/XhoI site of MSCV-IRES-GFP (Addgen) retroviral vector. pGIPZ lentiviral vector, pGIPZ-shCAMK2N1-1 (shCA-1, 5′-TCAATAACAACCCGCTTGC-3′), pGIPZ-shCAMK2N1-3 (shCA-3, 5′-TAGACACCAGGAGGTGCCT-3′) were purchased from Thermo Scientific. PSA-Luc MMTV-Luc, ARE4-Luc reporter genes were described [30 (link)]. AKT inhibitor VIII was purchased from Perkin Elmer (Waltham, MA). LNCaP infected with GIPZ-Vector, shCA-1, shCA-3. C4-2 infected with MSCV-IRES-GFP, MSCV-CAMK2N1-IRES-GFP, pGIPZ-Vector, shCA-1, shCA-3. GFP positive cells were selected by FACS (Fluorescence Activated cell sorter).

Human prostate cancer DU145 and PC3 cells were obtained from ATCC and maintained in DMEM medium (Invitrogen) supplemented with 10% fetal bovine serum. The CAMK2N1 human cDNA clone was purchased from OriGene Technologies and subcloned into the EcoRI/XhoI site of MSCV-IRES-GFP (Addgen) retroviral vector. pGIPZ Vector, pGIPZ-shCAMK2N1(V3LHS-315689, 5'-TCAATAACAACCCGCTTGC-3'), were purchased from Thermo Scientific. DU145 and PC3 cells infected with MSCV-IRES-GFP, MSCV-CAMK2N1-IRES-GFP, pGIPZ-Vector, pGIPZ-shCAMK2N1. GFP positive cells were selected by FACS (Fluorescence Activated cell sorter).