Log In Sign up
The largest database of trusted experimental protocols

Normal saline

Manufactured by Quality Biological
Visit Supplier

Normal saline is a sterile isotonic solution composed of sodium chloride and water. It is commonly used as a diluent, flushing agent, or replacement fluid in various medical and laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Bovine catalase, by Merck Group (1 mentions) Wiretrol, by Drummond (1 mentions) Microtainer tube, by BD (1 mentions) Bss plus, by Alcon (1 mentions) Lactated ringers, by Pfizer (1 mentions) Ultrapure distilled water, by Thermo Fisher Scientific (1 mentions) Sodium bicarbonate, by Merck Group (1 mentions) Sodium carbonate, by Merck Group (1 mentions)

4 protocols using normal saline

1

Catalase Assay for Bacterial Quantification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
We used the catalase assay developed by Iwase et al.16 (link) with slight modifications. Bacterial strains were grown overnight in 1% soytone at 37°C with shaking. Optical density at 600 nm (OD600) was recorded to estimate bacterial concentration and 2 mL of culture (6–8 × 109 bacteria) was centrifuged; the supernatant was removed; and the cell pellet was resuspended in 100 μL of normal saline (9 g/L sodium chloride; Quality Biological, Gaithersburg, MD) and transferred into 13 × 100 mm borosilicate glass tubes. One-hundred microliters of 1% Triton X-100 and 100 μL of 35% stabilized hydrogen peroxide were added. The tubes were left untouched at room temperature until the foaming stopped (about 2 minutes). The height of the foam in the tube was then recorded. A standard curve was also created using dilutions of commercially available bovine catalase (Sigma-Aldrich; 3,000 units/mg), and the final results were expressed as units of catalase (U) per 1010 bacterial cells, assuming an OD600 of 1.25 corresponds to 109 bacteria/mL of culture for all strains tested.
Fuche F.J., Sen S., Jones J.A., Nkeze J., Permala-Booth J., Tapia M.D., Sow S.O., Tamboura B., Touré A., Onwuchekwa U., Sylla M., Kotloff K.L, & Tennant S.M. (2017). Characterization of Invasive Salmonella Serogroup C1 Infections in Mali. The American Journal of Tropical Medicine and Hygiene, 98(2), 589-594.
+ Open protocol
+ Expand
2

Colon Sampling and Drug Administration in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Final dosing and processing conditions were as follows. Male 5–6 week old CF-1 mice were housed in cages with wire mesh bottoms to prevent coprophagia and food was withheld for 16–24 h to reduce the number of pellets present in the colon. Mice were anesthetized with isoflurane and given a 200 μL normal saline (Quality Biological) pre-cleansing enema administered with a plastic flexible feeding tube (Instech Laboratories; 22 G X 25 mm), which was optimized to be sufficient volume to clear the distal colon of pellets prior to dosing. Mice were allowed to regain consciousness for 10 min ambulatory time. Mice were then anesthetized to administer 50 μL of drug solution using a 100 μL WireTrol (Drummond) inserted at a depth of 2 cm into the mouse colon. At designated times, blood was collected into BD Microtainer tubes with K2EDTA, and plasma was obtained by centrifugation (1300 rcf for 10 min). The distal 4 cm of colon was excised, bisected open to remove pellets as necessary, and cut in half longitudinally with a scalpel. The tissue was stored in cryogenic tubes and flash frozen in liquid nitrogen. All samples were stored at −80˚C after being flash frozen in liquid nitrogen.
Hoang T., Date A.A., Ortiz J.O., Young T.W., Bensouda S., Xiao P., Marzinke M., Rohan L., Fuchs E.J., Hendrix C., Gumber S., Villinger F., Cone R.A., Hanes J, & Ensign L.M. (2018). Development of rectal enema as microbicide (DREAM): Preclinical progressive selection of a tenofovir prodrug enema. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 138, 23-29.
+ Open protocol
+ Expand
3

Thawing and Rinsing Cryopreserved REPS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen cryovials were maintained in liquid nitrogen for at least 1 day and up to 15 months. To thaw, cryovials were placed in a 37 °C water bath and REPS were removed from cryovials using fine-tipped forceps. Thawed REPS were rinsed in two sequential volumes (2 mL and 4 mL, respectively) of Normal Saline (Quality Biological), Lactated Ringers (Hospira), BSS (Alcon), or BSS PLUS (Alcon) at room temperature for approximately 20–30 s for the first rinse and approximately 1 min for the second rinse. Following the second rinse, REPS were transferred to X-VIVO 10 culture medium and maintained in a 37 °C incubator at 5% CO2.
Pennington B.O., Bailey J.K., Faynus M.A., Hinman C., Hee M.N., Ritts R., Nadar V., Zhu D., Mitra D., Martinez-Camarillo J.C., Lin T.C., Thomas B.B., Hinton D.R., Humayun M.S., Lebkowski J., Johnson L.V, & Clegg D.O. (2021). Xeno-free cryopreservation of adherent retinal pigmented epithelium yields viable and functional cells in vitro and in vivo. Scientific Reports, 11, 6286.
+ Open protocol
+ Expand
4

Compound Sourcing for HIV Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
TFV (FT104801501) and TDF (FT280311499) were both sourced from Carbosynth (Compton, UK). CMX157 (lot 021; potassium salt) was generously provided by Chimerix (Chimerix, Durham, NC). TAF (GS-7340; lot C11/019W) was provided by Gilead Sciences (Gilead, Foster City, CA). Normal saline was obtained from Quality Biological, Inc and UltraPure distilled water was obtained from Invitrogen. Sodium bicarbonate, sodium carbonate and sodium chloride powders were obtained from Sigma-Aldrich.
Hoang T., Date A.A., Ortiz J.O., Young T.W., Bensouda S., Xiao P., Marzinke M., Rohan L., Fuchs E.J., Hendrix C., Gumber S., Villinger F., Cone R.A., Hanes J, & Ensign L.M. (2018). Development of rectal enema as microbicide (DREAM): Preclinical progressive selection of a tenofovir prodrug enema. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 138, 23-29.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!