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28 protocols using 2 methylimidazole

1

Synthesis of LFP/C-ZIF-8 Composite

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The raw materials used in this experiment include methanol (99.5%, Beijing Chemical Works), zinc nitrate (99%, Tianjin Fuchen Chemical Reagent Factory), 2-methylimidazole (99%, Sinopharm Group Chemical Reagent Co., Ltd.), and commercial LiFePO4 powder (Qinghai Taifeng First Lithium Technology Co., Ltd.).
The coating processes were performed as described below: LFP was dispersed into methanol to form a uniform slurry by ultrasonic. Then, zinc nitrate and 2-methylimidazole were dissolved into the above slurry by magnetic stirring for 1 h. The mixture was aged at room temperature for 24 h and the gray powders were precipitated. The ratio of LFP dispersion: zinc nitrate: 2-methylimidazole was 100 mL:1.029 g:1.314 g. And the ratio of LFP: methanol was 8(g):x(mL) (x = 0, 188, 282, 376). The powders were washed very carefully with methanol and annealed at 500/600/700/800/900 °C with a heating rate of 5 °C min−1 under nitrogen flow to obtain the LFP/CZIF-8 samples. As the blank sample, the ZIF-8 was synthesized using the same method without LFP.
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2

Synthesis and Characterization of Metal-Organic Frameworks

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Zinc acetate dihydrate
(Zn(CH3COO)2·2H2O), 2-methylimidazole
(2MI), Sodium dichromate (Na2Cr2O7·2H2O), ethanol, acetone, and glutaraldehyde were
purchased from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China).
Phosphate buffer saline (PBS) was produced by Thermo Fisher Scientific
Co., Ltd. (Shanghai, China). The Luria–Bertani
broth was obtained from Solarbio Science & Technology Co., Ltd.
(Beijing, China). Bull aerum albumin-Rhodamine B isothiocyanate (BSA-RBITC)
was purchased from Beijing Bioss Biotechnology Co., Ltd. (Beijing,
China). EDTA solution (0.5 M, pH 8.0) and a reactive oxygen species
(ROS) assay kit were purchased from Shanghai Beyotime Technologies
Inc. (Shanghai, China). Fluorescein diacetate (FDA) and propidium
iodide (PI) double staining cell viability assay kit was from Shanghai
BestBio Biotechnology Co., Ltd. (Shanghai, China).
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3

Silver-Zinc Oxide Synthesis

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Silver nitrate (AgNO3, 99%), zinc chloride (ZnCl2, 99%), 2-methylimidazole (C4H6N2, 99%), rhodamine B (RhB, 99%) and methanol (CH3OH, 99%) were all analytical reagents and purchased from Sinopharm Chemical Reagent Co. Ltd. All reagents were directly used as received without further purification. Distilled water was used in this experiment.
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4

Synthesis of Cobalt-based Catalysts

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All of the chemicals for
syntheses used in this study were purchased commercially and used
as received. Cobaltous nitrate hexahydrate (Co(NO3)2·6H2O, ≥98.5%), 2-methylimidazole (2-MeIM,
≥99%), tetrahydrofuran (THF, ≥99%), phosphomolybdic
acid hydrate (H3PMo12O40·nH2O, ≥99.6%), and sodium molybdate dihydrate
(NaMoO4·2H2O, ≥99%) were purchased
from Sinopharm Chemical Reagent Co., while tetrahydrofuran (THF, ≥99%)
from Aladdin.
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5

Synthesis of Co3S4-Graphene Hybrid

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100 mg of GO was dispersed in 40 mL deionized water. Then 1.454 g of cobalt nitrate hexahydrate (Co(NO3)·6H2O, 98.5%, Sinopharm Chemical Reagent Co., Ltd) was added into the GO aqueous solution and stirred for 20 min. 2-Methylimidazole (2-mlm, 99%, Sinopharm Chemical Reagent Co., Ltd) was dispersed in another 60 mL deionized water and, after it completely dissolved, it was quickly added to the above mentioned mixture, and stirred for 30 min. The composite of Co-MOF and GO (denoted as Co-MOF–GO) was obtained after centrifugation and dried overnight at 60 °C. Then, Co-MOF–GO was mixed with sublimed sulfur (S, 99.5%, Sinopharm Chemical Reagent Co., Ltd) at a mass ratio of 1 : 3, then heated in a furnace filled with argon at 800 °C for 2 h (heating rate 5 °C min−1) to obtain the Co1−xS/Co9S8 and graphene hybrid (denoted as Co1−xS/Co9S8–G). Finally, the Co1−xS/Co9S8–G powder was annealed in a muffle furnace at 300 °C for 3 h (heating rate 2 °C min−1) to obtain Co3S4–G.
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6

Synthesis of Cobalt-based Materials

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Cobalt nitrate hexahydrate (Co(NO3)2·6H2O, > 99.8%) was purchased from Shanghai Titanchem Co. Ltd., and methanol (CH3OH, > 99.5%), cobalt sulfate heptahydrate (CoSO4·7H2O, > 99.8%), 2-methylimidazole (C4H6N2, 99%), and polyvinylpyrrolidone ((C6H9NO)n) were obtained from Sinopharm Chemical Reagent Co. Ltd. All reagents were used as received without further purification.
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7

Fabrication of Cotton-based Adsorbent

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Zinc nitrate hexahydrate, 2-methylimidazole, methanol, ethanol and tetrachloroethene were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 2-Hydroxyethyl acrylate (HEA) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Cotton fabric was purchased from the market. All reagents were used without further purification.
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8

Luteolin-based Cytotoxicity Evaluation

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Luteolin with a purity of >99.9% was purchased from Shanghai Aladdin Biochemical Technology Co. Ltd., China. Zinc nitrate hexahydrate (Zn(NO3)2·6H2O), 2-methylimidazole, dimethyl sulfoxide (DMSO) and methanol with analytical grade were obtained from Sinopharm Group Chemical Reagent Co. Ltd., China. Roswell Park Memorial Institute (RPMI)-1640 medium, fetal bovine serum (FBS), phosphate buffered saline (PBS), trypsin-EDTA and streptomycin were purchased from Solarbio Biotechnology Co., Ltd., China. Cell Counting Kit-8 acquired from Beyotime Biotechnology Co., Ltd., China. The other chemicals such as Tween 80, sodium hydroxide and phosphoric acid were of analytical grade and purchased from Aladdin Biochemical Technology Co. Ltd. China.
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9

Synthesis of Metal-Organic Frameworks

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Nickel nitrate hexahydrate (Ni(NO3)2·6H2O, 99.9%), methanol, 2-methylimidazole (98%) and zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%) were purchased from Sinopharm Chemical Reagent Co., Ltd, Shanghai, China. All chemical reagents were of analytical grade and were used without further purification.
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10

Quantification of Genetically Modified Proteins

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Streptavidin (SA), hemoglobin, horseradish peroxidase (HRP), bovine serum albumin (BSA), 3,3′,5,5′-tetramethylbenzidine (TMB) and polyvinyl pyrrolidone (PVP) were obtained from Sigma-Aldrich. 2-methylimidazole, zinc nitrate (Zn (NO3)2), tween-20, sulfuric acid (H2SO4), potassium dihydrogen phosphate (KH2PO4), dibasic sodium phosphate (Na2HPO4), potassium chloride (KCl) and sodium chloride (NaCl) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 96-well ELISA plates were bought from Corning-Costar. CP4-EPSPS, Cry1Ab, Cry1e, biotin-antibody and monoclonal antibody to Cry1Ab were acquired from Youlong Biotech Co., Ltd. (Shanghai, China). All chemical reagents were used as received without further purification.
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