RAS-selective lethal (RSL3, S8155) and ferrostatin-1 (Fer-1, S7243) were purchased from Selleck Chemicals (Shanghai, China) and dissolved in dimethyl sulfoxide (DMSO, V900090, Sigma-Aldrich). RSL3 and Fer-1 were prepared for intraperitoneal (i.p.) injection as follows: 1% RSL3 or Fer-1 + 30% PEG300 (S6704, Selleck) + 5% Tween 80 (S6702, Selleck) + 64% H2O. N-Acetyl-l -cysteine (NAC, A9165, Sigma-Aldrich) was used. An optimal cutting temperature compound (OCT, 14020108926) was obtained from Leica (Wetzlar, Germany).
Fer 1 s7243
Manufactured by Selleck Chemicals
Sourced in China, United States
Fer-1 (S7243) is a chemical compound used in laboratory settings. It serves as a functional tool for researchers and scientists. The core purpose of this product is to facilitate specific experimental or analytical procedures, without providing interpretations or recommendations on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Rsl3 s8155, by Selleck Chemicals (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Fer 1, by Selleck Chemicals (1 mentions)
Peg300, by Selleck Chemicals (1 mentions)
N acetyl l cysteine nac a9165, by Merck Group (1 mentions)
Acetylated lysine, by Cell Signaling Technology (1 mentions)
Phospho ser thr phe, by Cell Signaling Technology (1 mentions)
Erastin s7242, by Selleck Chemicals (1 mentions)
Ki67 sc15402, by Santa Cruz Biotechnology (1 mentions)
Ab253183, by Abcam (1 mentions)
Dcfh da, by Dojindo Laboratories (1 mentions)
Celltiter glo 3d cell viability assay, by Promega (1 mentions)
Propidium iodide, by Beyotime (1 mentions)
L cystine, by Merck Group (1 mentions)
D galn, by Merck Group (1 mentions)
Escherichia coli l 2630, by Merck Group (1 mentions)
α mem cell culture medium, by Thermo Fisher Scientific (1 mentions)
Plko 1 puro shrna vector, by Merck Group (1 mentions)
Erastin e7781, by Selleck Chemicals (1 mentions)
Ly2090314, by Selleck Chemicals (1 mentions)
7 protocols using fer 1 s7243
1
Preparation and application of RAS-selective lethal and ferroptosis inhibitors
2
Comprehensive Reagents for Cell Signaling
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The antibodies to DJ-1 (#5933), p-p38 (#9216), p38 (#8690), AHCYL1 (#94248), SLC7A11 (#12691), Acetylated-Lysine (#9441), and Phospho-(Ser/Thr) Phe (#9631) were obtained from Cell Signaling Technology. The primary antibody against NRF2 (sc13032), γ-GCS (sc390811), GSS (sc166882), SAHH (sc271389), and Ki67 (sc15402) were obtained from Santa Cruz Biotechnology. The primary antibody against GAPDH (db106), HA (db2603), and Flag (db7002) were obtained from Diagnostic Biosystems. GSH (G4251), NAC (A7250), DL-Met (M9500), L-glutamine (G3126), SAM (A4377), DL-homocysteine (H4628), SAH (A9384), and BSO (19176) were obtained from Sigma-Aldrich. Erastin (S7242), Fer-1 (S7243), and RSL3 (S8155) were obtained from Selleck Chemicals. Sorafenib (S125098) was obtained from Aladdin. ML210 (GC18705) was obtained from Glpbio. PE (HY100887) was obtained from MedChem Express. DMEM (GIBICO, #12800), RPMI-1640 (GIBICO, #31800), and DMEM without Met (#21013024) were obtained from Thermo Fisher Scientific.
3
Investigating Donafenib's Anticancer Mechanisms
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Donafenib was provided by Suzhou Zelgen Biopharmaceuticals Co, Ltd.; GSK‐J4 (HY‐15648B), sorafenib (HY‐10201A), regorafenib (HY‐10331), Z‐VAD‐FMK (HY‐16658), necrosulfonamide‐1 (HY‐100573), 3‐MA (HY‐19312), hemin (HY‐19424), BSO(HY‐106376), Tocopherol(HY‐131553), DFO(HY‐B0988) and zinc protoporphyrin (ZnPP; HY‐101193) were purchased from MCE; Fer‐1 (S7243) was purchased from Selleck; CCK‐8(C0005) was purchased from Targetmol; Liperfluo(L248), MDA Assay Kit(M496) and DCFH‐DA(R253) was purchased from Dojindo; CellTiter‐Glo® 3D Cell Viability Assay was purchased from Promega(G9681), propidium iodide (PI) was purchased from Beyotime; and FeRhoNoxTM‐1(GC901) was purchased from Goryo. Antibodies against KDM6A (ab253183), GPC3 (ab207080), NRF2 (ab62352), H3K4me1 (ab8895) and H3K27ac (ab4729) were purchased from Abcam; antibody against HA‐tag (HT301) was purchased from TransGen Biotech, antibodies against HMOX1 (10701‐1‐AP) and Actin (66009‐1‐Ig) and were purchased from Proteingroup; and antibody against H3K27me3 (07‐449) was purchased from Sigma. ATAC kit (N248) and CUT&Tag kit (N259) were purchased from novoprotein.
4
Cell Viability and Ferroptosis Assay
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RSL3 (S8155) and Fer-1 (S7243) were purchased from Selleck Chemicals (USA). iFSP1 (HY-136057) was purchased from MedChemExpress (USA). C-11 BODIPY 581/591 (InvitrogenTM, D3861) and L-Glutamine solution 200 mM (GibcoTM, 25030081) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). L-Methionine (M5308) and L-Cystine (C7602) were purchased from Sigma-Aldrich (USA). All dissolved reagents were stored at −20 °C until use.
5
Hepatic Conditional TGFβr1 Knockout Mouse Model for Acute Liver Failure
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Male C57BL/6 WT, Nrf2-/- (strain Nrf2tm1Ywk/J), B6.129-Albtm1(CreERT2)Srcmo, TGFβr1flox/flox (Jackson Laboratory, Bar Harbor, ME) mice between 9 and 12 weeks of age were genotyped, housed under specific pathogen-free conditions. Tamoxifen-induced, hepatic-specific conditional TGFβr1 knock-out mice were generated by TGFβr1flox/flox and B6.129-Albtm1(CreERT2)Srcmo mice. Tamoxifen (20 mg/mL) was administered via intraperitoneal injection once every 24 hours for a total of 5 consecutive days to induce hepatic-specific conditional TGFβr1 knock-out mice (TGFβr1Δhep-CKO).
The murine model of ALF was characterized previously. Adult male mice weighing 20–22 g were injected intraperitoneally with LPS (20 μg/kg, Escherichia coli, L2630; Sigma, Saint Louis, MO) in 100 μL saline 15 minutes before injection of D-GalN (700 mg/kg, G0500; Sigma, Saint Louis, MO) in 100 μL saline. Mice were killed 6 hours after LPS and D-GalN administration. ZVAD-FMK (S7023), DFOM (S5742), and Fer-1 (S7243) inhibitors were purchased from Selleck (Houston, TX). All the procedures used in the animal studies were approved by the National Institutional Animal Care and Ethical Committee of Southern Medical University. All the experimental methods performed in this study were by the approved guidelines.
The murine model of ALF was characterized previously. Adult male mice weighing 20–22 g were injected intraperitoneally with LPS (20 μg/kg, Escherichia coli, L2630; Sigma, Saint Louis, MO) in 100 μL saline 15 minutes before injection of D-GalN (700 mg/kg, G0500; Sigma, Saint Louis, MO) in 100 μL saline. Mice were killed 6 hours after LPS and D-GalN administration. ZVAD-FMK (S7023), DFOM (S5742), and Fer-1 (S7243) inhibitors were purchased from Selleck (Houston, TX). All the procedures used in the animal studies were approved by the National Institutional Animal Care and Ethical Committee of Southern Medical University. All the experimental methods performed in this study were by the approved guidelines.
6
Biochemical Assays for Cell Death
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PA and CTL BSA were purchased from Kunchuang Biotechnology (Xian, China). ZnPP (HY-101193) was purchased from MedChemExpress (Shanghai, China). STZ (S1312), Z-VAD-FMK (S7023), VitE (S4686), Nec-1 (S8037), 3-MA (S2767), DFO (S5742), and Fer-1 (S7243) were purchased from Selleck Chemicals (Shanghai, China). α-MEM cell culture medium and fetal bovine serum (FBS) were purchased from Gibco (USA).
7
Genetic manipulation of GSK-3β in cell lines
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All cell lines (HeLa, MDA-MB-231, and HEK293T) were obtained from the American Type Culture Collection and were maintained in Dulbecco’s modified Eagle medium (DMEM, Gibco, 8121298) supplemented with 10% fetal bovine serum (FBS, Gibco, 2176398) and 1% penicillin–streptomycin at 37 °C with 5% CO2.
The following regents were used in this study: Erastin (E7781), Fer-1 (S7243), and LY2090314 (S7063) were purchased from Selleck (Houston, TX, USA). The coding region of GSK-3β gene was cloned into the pCMV vector in-frame with a myc tag within the vector. The pLKO.1-puro shRNA vector was obtained from Sigma-Aldrich (SHC001). The pLKO.1-shGSK-3β 1# and 2# were constructed using specific primer containing separate targeting sequence. (targeting sequence to GSK-3β 1#: 5′-CACTGGTCACGTTTGGAAAGA-3′, GSK-3β 2#: 5′-CCCAAACTACACAGAATTTAA-3′).
The following regents were used in this study: Erastin (E7781), Fer-1 (S7243), and LY2090314 (S7063) were purchased from Selleck (Houston, TX, USA). The coding region of GSK-3β gene was cloned into the pCMV vector in-frame with a myc tag within the vector. The pLKO.1-puro shRNA vector was obtained from Sigma-Aldrich (SHC001). The pLKO.1-shGSK-3β 1# and 2# were constructed using specific primer containing separate targeting sequence. (targeting sequence to GSK-3β 1#: 5′-CACTGGTCACGTTTGGAAAGA-3′, GSK-3β 2#: 5′-CCCAAACTACACAGAATTTAA-3′).
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