Fluconazole

Seven isolates of C. albicans (SC5314, CA4, CA8, CA10, CA16, CA103 and CA137) were used in this study, which were isolated from the blood of patients and kindly provided by Professor Shujuan Sun (Shandong Provincial Qianfoshan Hospital, Jinan, China). C. albicans ATCC 10231 was used as a reference strain of the study. All isolates were taken from previously established stocks. Before all experiments, isolates were routinely inoculated twice in yeast extract-peptone-dextrose agar medium overnight at 35°C. Teriflunomide (Dalian Meilun Biotech Co., Ltd, Dalian, China) was dissolved in ethyl alcohol at a concentration of 54, 000 μg/mL, whereas fluconazole (Dalian Meilun Biotech Co., Ltd, Dalian, China) was dissolved in sterilized water at a concentration of 5, 120 μg/mL. To avoid affecting the outcomes of experiments, the proportion of ethyl alcohol was less than 1% of the whole test volume, according to the Clinical and Laboratory Standards Institute M27-A3 (CLSI M27-A3) (Rex et al., 2008 ). This study was approved by the Scientific Research Ethics Committee of Shandong Provincial Maternal and Child Health Hospital (No. 2023-083).

All reactions were carried out under an argon atmosphere. Chloroform was distilled under nitrogen from calcium hydride. 1,10-Phenanthroline was sublimated before use. Ampicillin and fluconazole were brought from Meilun Biotech Co. (Dalian, China), morpholinepropanesulfonic acid, and Salmon Sperm DNA were obtained from Sigma-Aldrich (St. Louis, MO, USA). Their stock solutions were freshly prepared, filter-sterilized, and used at indicated concentrations. SiI₄ was purchased from Alfa, and the other reagents were purchased from Sigma, Acros, or Strem and used without further purification. Column chromatography was performed with silica gel (230–400 mesh). ¹H- and ¹³C-NMR spectra were recorded on a Bruker Advance (400 MHz, Bruker Corporatio, Billerica, MA, USA) at ambient temperature.
In total, the following bacterial and fungal strains were used for this study: Cryptococcus neoformans H99, Candida albicans ATCC90028, Staphylococcus aureus ATCC 33591(MRSA), ATCC 25923(MSSA), Escherichia coli ATCC25922, Pseudomonas aeruginosa PAO1, and clinical isolated Acetobacter baumannii. S. aureus, E. coli, and P. aeruginosa were grown in TSB medium; C. neoformans and C. albicans were grown in YPD medium. These strains were stored as glycerin stock in −80 °C.