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Anti sumo1

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Anti-SUMO1 is a laboratory reagent used in research applications. It is a specific antibody that recognizes the SUMO1 protein, which is involved in post-translational modification processes in cells. The core function of Anti-SUMO1 is to enable the detection and study of SUMO1 and SUMO1-related cellular processes.

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Lab products found in correlation

Anti sumo 2 3, by Enzo Life Sciences (3 mentions) Anti gst, by Cell Signaling Technology (2 mentions) Anti teneurin1, by Novus Biologicals (2 mentions) Anti mor ct aa 387 398, by GenScript (2 mentions) Anti actin, by Merck Group (1 mentions) Anti pml, by Fortis Life Sciences (1 mentions) Anti sumo1, by Thermo Fisher Scientific (1 mentions) Anti sumo2 3, by Abcam (1 mentions) Peroxidase conjugated anti mouse igg, by Merck Group (1 mentions) Anti σ1r, by Thermo Fisher Scientific (1 mentions) Anti cam, by Merck Group (1 mentions) Pierce crosslink magnetic ip co ip kit, by Thermo Fisher Scientific (1 mentions) Anti calmodulin, by Merck Group (1 mentions)

4 protocols using anti sumo1

1

Characterization of SUMO Pathway Proteins

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Primary rabbit antibodies included anti-actin (Sigma-Aldrich; A5060), anti-Daxx (Upstate; 07-471), anti-PIAS1 (LsBio; LS-B9173), anti-PIAS2 (LsBio; LS-C108717), anti-PIAS3 (LsBio; LS-C98795), anti-PIAS4 (LsBio; LS-C108719), anti-PML (Bethyl Laboratories; A301-167A), and anti-Sp100 (SpGH [37 (link)]) antibodies. Primary mouse antibodies included anti-ICP0 (11060 [38 (link)]), anti-ICP4 (58S [39 (link)]), anti-Daxx (AbD Serotec; MCA2143), anti-SUMO1 (Invitrogen; 33-2400), anti-SUMO2/3 (Abcam; ab81371), anti-UL42 (Z1F11 [40 (link)]), and anti-VP5 (DM165 [41 (link)]) antibodies. Primary sheep antibodies included anti-SUMO1 (Enzo Life Sciences; BML-PW0505) and anti-SUMO2/3 (Enzo Life Sciences; BML-PW0510) antibodies. Secondary antibodies included peroxidase-conjugated anti-mouse IgG (Sigma-Aldrich; A4416), DyLight 680- or DyLight 800-conjugated goat anti-rabbit or -mouse IgG (Thermo), and Alexa Fluor 488-, 555-, or 633-conjugated donkey anti-rabbit, -sheep, or -mouse IgG antibodies (Invitrogen).
Brown J.R., Conn K.L., Wasson P., Charman M., Tong L., Grant K., McFarlane S, & Boutell C. (2016). SUMO Ligase Protein Inhibitor of Activated STAT1 (PIAS1) Is a Constituent Promyelocytic Leukemia Nuclear Body Protein That Contributes to the Intrinsic Antiviral Immune Response to Herpes Simplex Virus 1. Journal of Virology, 90(13), 5939-5952.
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2

Antibody Detection of Immunoprecipitated Receptor

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The primary antibody to detect immunoprecipitated receptor was: anti-MOR Ct aa 387–398 (GenScript Co.). Other primary antibodies used in this study were: anti-CaM (Millipore; #05-173), anti-GST (Cell Signaling; #2622), anti-HINT2 (Abnova; #H00084681-01), anti-RGSZ2(1 (link)) (Thermo Scientific; #PA1-25695), anti-RGSZ2(2 (link)) (aa 192-215; GenScript Co.) (14 (link)), anti-teneurin1 (Novus Biologicals; #NBP2-41315), anti-σ1R (Invitrogen; #42-3300), anti-SUMO1 (Enzo; #BML-PW9460), and anti-SUMO-2/3 (Enzo; #BML-PW9465). The anti-HINT1 antibody was raised in rabbits (Immunostep) against the peptide sequence GYRMVVNEGADGGG (93–106). All primary antibodies were detected by using the appropriate horseradish peroxidase-conjugated secondary antibodies.
Cortés-Montero E., Rodríguez-Muñoz M., Sánchez-Blázquez P, & Garzón J. (2019). The Axonal Motor Neuropathy-Related HINT1 Protein Is a Zinc- and Calmodulin-Regulated Cysteine SUMO Protease. Antioxidants & Redox Signaling, 31(7), 503-520.
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3

Investigating HCMV UL44 Sumoylation

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HFF cells were infected with wild-type HCMV (UL44WT-Zeo) or HCMV containing UL44 K410A mutant (UL44-K410A-Zeo) at an MOI of 1. At 48 h post-transfection, total cell lysates were either directly analyzed by SDS-PAGE, or immunoprecipitated with anti-UL44 antibody which was covalently immobilized on Protein A/G beads by Pierce Crosslink Magnetic IP/Co-IP Kit (Thermo Fisher Scientific), then probed for UL44 or sumoylated proteins using anti-UL44 (Santa Cruz Biotechnology) or anti-SUMO1 (Enzo Life Science) antibodies, respectively.
Chen J., Li G., He H., Li X., Niu W., Cao D, & Shen A. (2021). Sumoylation of the Carboxy-Terminal of Human Cytomegalovirus DNA Polymerase Processivity Factor UL44 Attenuates Viral DNA Replication. Frontiers in Microbiology, 12, 652719.
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4

Detecting Recombinant Protein Targets

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The primary antibodies to detect the recombinant proteins were anti-MOR Ct aa 387-398 (GenScript Co.), anti-Calmodulin (#05-173, Millipore,), anti-GST (#2622, Cell Signaling), anti-RGSZ2(1) (#PA1-25695, Thermo Scienti c), anti-RGSZ2(2) (aa 192-215; GenScript Co), anti-teneurin1 (#NBP2-41315, Novus Biologicals), anti-SUMO1 (#BML-PW9460, Enzo), anti-SUMO-2/3 (#BML-PW9465, Enzo). The anti-HINT1 antibody was raised in rabbits (Immunostep) against the peptide sequence GYRMVVNEGADGGG (93-106). All primary antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies.
Cortés-Montero E., Rodríguez-Muñoz M., Sánchez-Blázquez P, & Garzón-Niño J. (2021). Human HINT1 Mutant Proteins that Cause Axonal Motor Neuropathy Exhibit Anomalous Interactions with Partner Proteins. Molecular neurobiology, 58(4).
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