A quantitative and statistical analysis of immunohistochemical reaction using the Olympus DP-Soft image system (version 4.1 for Windows) was carried out on a Microphot-SA (Nikon, Japan) microscope equipped with a Camedia-5050 Z digital camera (Olympus, Japan). The analysis was performed on sections from the SM strips from the stomach of Wistar rats (n = 6 for each group). Five sections of the SM strips were measured, and the percentage of cells expressing 5-HT3 in the circular and longitudinal layer of SM cells, as well as in the myenteric plexus of the stomach, was determined. Each antibody was analyzed for five fields, in each of them the average number of cells with positive unit area response at ×200 magnification.
Camedia 5050z digital camera
Manufactured by Olympus
Sourced in Japan
The Camedia-5050Z is a digital camera manufactured by Olympus. It has a 5.0-megapixel image sensor and can capture images at a maximum resolution of 2,560 × 1,920 pixels.
Automatically generated - may contain errors
Lab products found in correlation
Dp soft image system, by Nikon (3 mentions)
Microphot sa, by Nikon (3 mentions)
Microphot sa microscope, by Nikon (2 mentions)
A2547, by Merck Group (2 mentions)
Bbk 120, by ScyTek Laboratories (1 mentions)
Super block, by ScyTek Laboratories (1 mentions)
Sc 12187, by Santa Cruz Biotechnology (1 mentions)
7 protocols using camedia 5050z digital camera
1
Immunohistochemical Analysis of Stomach Muscles
2
Immunohistochemical Analysis of ACE2 and αSMA in Rat Heart
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The sections (5 µm-thick) obtained from rat heart were deparaffinized, then subjected to the following procedures: detection of antigenic epitopes with citrate buffer, blocking endogenous peroxidase with 3% hydrogen peroxide, blocking endogenous biotin using a kit (ref: № BBK 120, Scy Tek, Lab. Inc., Logan, UT, USA), blocking non-specific binding using a reagent (Superblock, Scy Tek, Lab. Inc., Logan, UT, USA), followed by incubation for 24 h (at 4 °C) with ACE2 polyclonal antibody 1:200 (E-AB-12224, Elabscience Biotechnology Inc., Houston, TX, USA), after which a second 10 min incubation followed, with a biotinylated secondary antibody (№ AGL015 Scy Tek Lab. Inc., Logan, UT, USA). The reaction was visualized by 3,3′-diaminobenzidine tetrachloride (DAB, Scy Tek Lab. Inc., Logan, UT, USA), and the slices were counterstained with Mayer’s hematoxylin. The same protocol was used for the immunohistochemical analysis of αSMA monoclonal antibody 1:5000 (A-2547, Sigma Chemical, St. Louis, MO, USA). All microphotographs were taken using a Nikon Microphot SA microscope (Olimpus, Japan), combined with Camedia-5050Z digital camera (Olympus, Japan).
3
Histological Analysis of Rat Aortas
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Descending thoracic aortas from CY, CO, and A groups of rats were harvested and fixed in 10% formalin for 48 hours and then transferred to 70% ethanol for storage at 4°C. Fixed aortic segments were embedded in paraffin and sectioned at 5 μm thickness. Sequential sections were stained with hematoxylin/eosin and orcein. The photomicrographs enclosed were taken on Nikon Microphot SA microscope (Japan), equipped with a Camedia-5050Z digital camera (Olympus, Japan).
4
Immunohistochemical Analysis of Gastrointestinal Radiation Damage
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Immunohistochemical studies were performed on paraffin-embedded sections (4–5 µm) obtained from tissues of control and irradiated rats (n = 6), mounted on glass microscope slides and secured by means of an adhesive.
The sections obtained from the gastric wall of the rat were deparaffinized, then subjected to the following procedures: detection of antigenic epitopes with citrate buffer, blocking endogenous peroxidase with 3% hydrogen peroxide, blocking endogenous biotin using a kit (ref: No. BBK 120, Scy Tek, Lab., Inc., Logan, UT, USA), blocking non-specific binding using a reagent (Superblock, Scy Tek, Lab., Inc., Logan, UT, USA), followed by incubation for 24 h (at 4 °C) with specific mouse monoclonal antibody against SR-2A (A-4): sc-166775 or SR-2B (C-6): sc-376878 (Santa Cruz Biot., CA, USA, 1:300 solution), after which a second 10 min incubation followed, with a biotinylated secondary antibody (No. AGL015 Scy Tek Lab., Inc., Logan, UT, USA). The reaction was visualized by 3,3′-diaminobenzidine tetrachloride (DAB, Scy Tek Lab., Inc., Logan, UT, USA), and the slices were counterstained with Mayer’s hematoxylin.
All microphotographs were taken using a Nikon Microphot SA (Nikon, Tokyo, Japan) microscope combined with a Camedia-5050Z digital camera (Olympus, Tokyo, Japan). The preparations were observed at a magnification ×400.
The sections obtained from the gastric wall of the rat were deparaffinized, then subjected to the following procedures: detection of antigenic epitopes with citrate buffer, blocking endogenous peroxidase with 3% hydrogen peroxide, blocking endogenous biotin using a kit (ref: No. BBK 120, Scy Tek, Lab., Inc., Logan, UT, USA), blocking non-specific binding using a reagent (Superblock, Scy Tek, Lab., Inc., Logan, UT, USA), followed by incubation for 24 h (at 4 °C) with specific mouse monoclonal antibody against SR-2A (A-4): sc-166775 or SR-2B (C-6): sc-376878 (Santa Cruz Biot., CA, USA, 1:300 solution), after which a second 10 min incubation followed, with a biotinylated secondary antibody (No. AGL015 Scy Tek Lab., Inc., Logan, UT, USA). The reaction was visualized by 3,3′-diaminobenzidine tetrachloride (DAB, Scy Tek Lab., Inc., Logan, UT, USA), and the slices were counterstained with Mayer’s hematoxylin.
All microphotographs were taken using a Nikon Microphot SA (Nikon, Tokyo, Japan) microscope combined with a Camedia-5050Z digital camera (Olympus, Tokyo, Japan). The preparations were observed at a magnification ×400.
5
Quantitative Analysis of 5-HT3 Expression
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A quantitative and statistical analysis of immunohistochemical reaction using the Olympus DP-Soft image system (version 4.1 for Windows) was carried out on a Microphot-SA (Nikon, Tokyo, Japan) microscope equipped with a Camedia-5050Z digital camera (Olympus, Tokyo, Japan). The analysis was performed on sections from the SM strips from the stomach of Wistar rats (n = 6 for each group). Five sections of the SM strips were measured, and the percentage of cells expressing 5-HT3 in the circular and longitudinal layer of SM cells, as well as in the myenteric plexus of the stomach, was determined. Each antibody was analyzed for five fields, in each of them the average number of cells with positive unit area response at ×400 magnification.
6
Immunohistochemical Analysis of ACE and SMA
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Sections were deparaffinised, then subjected to antigenic detection of the epitopes with citrate buffer, and an endogenous peroxidase blockade was made with hydrogen peroxidase (3%), a kit (ref: No. BBK 120, Scy Tek, USA) was used to block the endogenous biotin аnd a reagent to block non-specific binding (Superblock, Scy Tek), followed by incubation for 24 hours at 4°C with anti-goat АСЕ -1:300 (sc-12187, Santa Cruz Biotechnology Inc. USA) and monoclonal anti-α smooth muscle actin (A-2547, Sigma) 1:5000 -NRS/TBS/BSA, next incubated with secondary antibody: biotinylated anti-goat (No. AGL015 Scy Tek., USA) for 10 min. The reaction was visualized with 3,3΄-diaminobenzidine tetrachloride (DAB, ScyTek Lab. Inc., USA); counterstaining was performed with Mayer's hematoxylin. As negative controls, sections in which the primary antibodies were replaced by a buffer solution (PBS) were used. Microphotographs were performed with Nikon Microphot SA microscope (Japan), combined with Camedia-5050Z digital camera (Olympus, Japan) at ×100 and ×400 magnification.
7
Immunohistochemical Analysis of Rat Testes
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Quantitative analysis of the immunohistochemical reaction was conducted using the Olympus DP-Soft image system (version 4.1 for Windows) and Microscope-SA (Nikon, Japan) system equipped with Camedia-5050Z digital camera (Olympus, Japan). The analysis was performed on sections from testis of Wistar rats (n=25 for each group of animals, controls, and the group fed the high lipid diet). The parameters were measured on 5 sections of the animal; we determined the percentage of cells expressing CRP, SAA, IL-4, and ACE in the seminiferous tubules and the testicular interstitial cells. For each antibody was analyzed for 5 fields of the testis of each animal in each of them by means of a graduated grating (6×5 fields, each field having a size of 100 μm 2 ) was determined average number of cells with positive unit area response at ×400 magnification.
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