The levels of the CD44+, CD24 -, and CD133+cells in each group were evaluated using flow cytometry with a fluorescein isothiocyanate (FITC)-conjugated CD44 antibody (cat. no. 347943; BD Biosciences), phycoerythrin (PE)-conjugated CD24 antibody (cat. no. 555428; BD Biosciences), and a PE-conjugated CD133 antibody (cat. no. 12-1331-82; eBioscience; Thermo Fisher Scientific, Inc.). Mouse immunoglobulin (Ig)G2a-FITC (cat. no. 555573; BD Biosciences), mouse IgG2a-PE (cat. no. 554648; BD Biosciences), and mouse IgG1K-PE (cat. no. 12-4714-42; eBiosciences Thermo Fisher Scientific, Inc.) were used as the isotype controls (all of antibodies diluted at 1:100 ratio). Cells were incubated with antibodies at 4˚C for 45 minutes and analyzed using FACSCaliburTM (Becton Dickinson). The raw data were analyzed using Flowing Software version 2.5.0 (Perttu Terho).
Mouse igg1k pe
Manufactured by BD
Mouse IgG1K-PE is a mouse immunoglobulin G1 kappa (IgG1K) conjugated with the fluorescent dye Phycoerythrin (PE). It is a laboratory reagent used for various applications in flow cytometry and cell biology research.
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Lab products found in correlation
2 protocols using mouse igg1k pe
1
Quantifying Cancer Stem Cell Markers
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The levels of the CD44+, CD24 -, and CD133+cells in each group were evaluated using flow cytometry with a fluorescein isothiocyanate (FITC)-conjugated CD44 antibody (cat. no. 347943; BD Biosciences), phycoerythrin (PE)-conjugated CD24 antibody (cat. no. 555428; BD Biosciences), and a PE-conjugated CD133 antibody (cat. no. 12-1331-82; eBioscience; Thermo Fisher Scientific, Inc.). Mouse immunoglobulin (Ig)G2a-FITC (cat. no. 555573; BD Biosciences), mouse IgG2a-PE (cat. no. 554648; BD Biosciences), and mouse IgG1K-PE (cat. no. 12-4714-42; eBiosciences Thermo Fisher Scientific, Inc.) were used as the isotype controls (all of antibodies diluted at 1:100 ratio). Cells were incubated with antibodies at 4˚C for 45 minutes and analyzed using FACSCaliburTM (Becton Dickinson). The raw data were analyzed using Flowing Software version 2.5.0 (Perttu Terho).
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The levels of the CD44+, CD24 -, and CD133+cells in each group were evaluated using flow cytometry with a fluorescein isothiocyanate (FITC)-conjugated CD44 antibody (cat. no. 347943; BD Biosciences), phycoerythrin (PE)-conjugated CD24 antibody (cat. no. 555428; BD Biosciences), and a PE-conjugated CD133 antibody (cat. no. 12-1331-82; eBioscience; Thermo Fisher Scientific, Inc.). Mouse immunoglobulin (Ig)G2a-FITC (cat. no. 555573; BD Biosciences), mouse IgG2a-PE (cat. no. 554648; BD Biosciences), and mouse IgG1K-PE (cat. no. 12-4714-42; eBiosciences Thermo Fisher Scientific, Inc.) were used as the isotype controls (all of antibodies diluted at 1:100 ratio). Cells were incubated with antibodies at 4˚C for 45 minutes and analyzed using FACSCaliburTM (Becton Dickinson). The raw data were analyzed using Flowing Software version 2.5.0 (Perttu Terho).
2
PD-L1 Expression in A375 Cells
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A375 parental cells and A375 isogenic cells were harvested with non-enzymatic cell dissociation solution, washed once in ice cold PBS, and re-suspended in flow cytometry staining buffer at 1 × 106 cells per mL. Then 200 μL of each cell type was stained with 20 μL R-phycoerythrin (PE) conjugated mouse anti-human CD274 (PD-L1) antibody (BD Pharmingen, 557924) for 30 min. Mouse IgG1k-PE (BD Pharmingen, 555749) was as an isotype control. Stained cells were analyzed by flow cytometry. Where indicated, cells were stimulated overnight with 200 ng/mL recombinant human interferon gamma (R & D Systems, 285-IF) before they were harvested for flow cytometry analysis.
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