Sodium carbonate decahydrate

For the macronutrient analysis all analytical grade reagents were purchased from Panreac (Barcelona, Spain) and Merck (Darmstadt, Germany). Tocopherols (␣, ␤, ␥ and ␦) and tocotrienols (␣, ␤, ␥ and ␦) were purchased from Calbiochem (La Jolla, California, USA) and tocol (2-methyl-2-(4,8,12-trimethyl-tridecyl)chroman-6-ol) was obtained from Matreya Inc. (Pennsylvania, USA). The mixture of methyl esters of fatty acids (FAME) standards Supelco37 were obtained from Supelco (Bellefonte, PA, USA). 1,1-diphenyl-2-picrylhydrazyl (DPPH • ) free radical, Folin-Ciocalteu's reagent, gallic acid, glycerol, TPTZ (2,4,6-tripyridyl-s-triazine) solution, petroleum ether, potassium hydroxide, anhydrous sodium sulfate, ferrous sulfate heptahydrate were purchased from Sigma-Aldrich (Steinheim, Germany). Ethanol reagent grade, sodium acetate, sodium carbonate decahydrate, sodium nitrite, aluminum chloride, acetone, n-hexane, methanol and sodium hydroxide were purchased from Merck (Darmstadt, Germany). HPLC-grade n-hexane and 1,4-dioxane were from Fluka (Madrid, Spain). HPLC grade nhexane was from Merck (Darmstad, Germany). Purified water was obtained from a Milli-Q water purification system (Millipore, Bedford, MA, USA).