Victor 1420 multilabel counter

Manufactured by PerkinElmer

Sourced in United States, Finland, Poland, Spain, Germany

The Victor 1420 multilabel counter is a versatile laboratory instrument designed for high-throughput detection and quantification of various assays. It is capable of performing fluorescence, luminescence, and absorbance measurements in microplates. The Victor 1420 features automated sample handling, temperature control, and multiple detection modes to support a wide range of applications in life science research and drug discovery.

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Lab products found in correlation

Celltiter glo, by PerkinElmer (4 mentions) Celltiter glo, by Promega (4 mentions) Celltiter glo assay, by Promega (3 mentions) Brdu kit, by Roche (3 mentions) Tmb single solution, by Thermo Fisher Scientific (3 mentions) Micro bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Lysis buffer, by Thermo Fisher Scientific (2 mentions) Lowry assay, by Bio-Rad (2 mentions) Lsrii flow cytometer, by BD (2 mentions) 4mu n acetyl β d glucosaminide, by Merck Group (2 mentions) Enhancement solution, by PerkinElmer (2 mentions) Anti cd69 clone h1.2f3, by BioLegend (2 mentions) Anti cd25, by BioLegend (2 mentions) Streptavidin europium, by PerkinElmer (2 mentions) Anti cd3ε fitc clone 145.2c11, by BioLegend (2 mentions) Anti cd4 percp clone rm4 5, by BioLegend (2 mentions) Human reg4 elisa pair set, by Sino Biological (2 mentions) Polypropylene conical tube, by BD (2 mentions) Thioflavin t tht, by Merck Group (2 mentions) Rabbit anti his hrp, by Thermo Fisher Scientific (2 mentions)

79 protocols using victor 1420 multilabel counter

CIP2A promoter activity assay

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Tcam2-cells were double transfected using the Surefect transfection reagent according to manufactures protocol (Nunclon Surface, Nunc). Cells were transfected with CIP2A-promoter construct (1802 bp upstream [38 (link)], renilla plasmid and siRNA (either scrambled or siOct4-1). Promoter construct (200 ng), renilla (10 ng) and 2 pmol of siRNA were transfected per 96 well plate. Transfections with −1802 bp, −865 bp and −1802ΔCIP2ALuc CIP2A promoter constructs were also done as described above only without siRNAs. −1802ΔCIP2ALuc construct was produced by GenScript mutagenesis service from −1802CIP2ALuc promoter construct and resulting promoter sequence was validated by DNA sequencing. After 3 days the promoter activity was measured using Promega's Dual-Glo luciferase Assay system (E2940) according to manufactures protocol. Luminescence was measured with Victor-multilabel counter 1420 (PerkinElmer).

Ventelä S., Sittig E., Mannermaa L., Mäkelä J.A., Kulmala J., Löyttyniemi E., Strauss L., Cárpen O., Toppari J., Grénman R, & Westermarck J. (2014). CIP2A is an Oct4 target gene involved in head and neck squamous cell cancer oncogenicity and radioresistance. Oncotarget, 6(1), 144-158.

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Quantifying Cell Density via Picogreen Assay

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Cell density was also estimated by means of a Quant-iT™ PicoGreen ® dsDNA Assay Kit (Invitrogen). Briefly, after 7 days, samples were washed with PBS and frozen in a -80ºC freezer. Once thawed, samples were digested with Proteinase K (Roche) diluted 1 to 20 in DPBS at pH 8.1 for 16 h under gently shaking; next, the enzyme was inactivated for 10 min at 90ºC. As soon as they were tempered, the samples were vortexed for 1 min and centrifuged for 1 min at 650 g. Next, 28.7 µL of the supernatants and standards were mixed with 28.52 µL of the Picogreen reagent at a 1:200 concentration in Tris-EDTA (TE) buffer, and 100 µL more of TE buffer was added to each well in a 96-well plate. After 5 min of incubation, fluorescence was measured in a Victor Multilabel Counter 1420 spectrophotometer (Perkin Elmer, Waltham, MA, USA) at 535 nm.

Arnal-Pastor M., Martínez-Ramos C., Vallés-Lluch A, & Pradas M.M. (2016). Influence of scaffold morphology on co-cultures of human endothelial and adipose tissue-derived stem cells. Journal of biomedical materials research. Part A, 104(6).

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Controlled Release of BSA from PEA-HA Scaffolds

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Bovine serum albumin (BSA) was used as a protein model to evaluate the potential of PEA scaffolds coated with HA as a controlled release system. Each sample (previously swollen in PBS) was loaded overnight at 37ºC in 1.2 mL of a 1 mg/mL BSA (Thermo Scientific) solution in PBS. After absorption, samples were transferred to 1.2 mL of fresh PBS each to follow the protein release. The supernatants were collected at selected time points (up to 240 h) and replaced with fresh PBS tempered at 37ºC. Three replicates per time, material and treatment were measured.
The BSA concentration in each supernatant was determined with a Micro BCA Protein assay kit (Thermo Scientific) following the manufacturer's instructions, and its absorbance was read with a Victor Multilabel Counter 1420 spectrophotometer (Perkin Elmer, Waltham, MA; USA) at 570 nm.

Arnal-Pastor M., Pérez-Garnes M., Monleón Pradas M, & Vallés Lluch A. (2016). Topologically controlled hyaluronan-based gel coatings of hydrophobic grid-like scaffolds to modulate drug delivery. Colloids and surfaces. B, Biointerfaces, 140.

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Sustained FITC-IgG Release from pG_EAK/EAK Hydrogel

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The release kinetics of a fluorescein (FITC)-labeled IgG (Pierce goat anti-human IgG) from pG_EAK/EAK gel were determined by monitoring the cumulative fluorescence in release media over 3 months. IgG-loaded gels were injected at the bottom of MicroSert (inner diameter = 4.15 mm, maximum volume = 500 μL) by instilling a mixture of EAK (60 nmol), pG_EAK (150 pmol) and IgG^FITC (75 pmol). The 55 μL mixture was incubated overnight at 37 °C before adding 400 μL of PBS containing 0.1% BSA, 0.024% NaN₃ as the release medium. The insert was placed in its receptacle vial and capped to prevent the medium evaporation. The vial was put under 37 °C for incubation. At various time points, 200 μL of the release medium were collected for measurement using plate reader (Perkin Elmer 1420 Multilabel Counter VICTOR™, excitation = 485 nm; emission = 535 nm). Aliquots of fresh release medium were added to restore the system to its initial volume. The apparent diffusivity (D_app) of IgG in pG_EAK/EAK gel was estimated by fitting the initial release to the non-steady state Fickian equation, relating M_t/M_∞ as a function of t^0.5.

Liu W., Wong-Noonan S., Pham N.B., Pradhan I., Spigelmyer A., Funk R., Nedzesky J., Cohen H., Gawalt E.S., Fan Y, & Meng W.S. (2019). A genetically engineered Fc-binding amphiphilic polypeptide for congregating antibodies in vivo. Acta biomaterialia, 88, 211-223.

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Cytotoxicity Evaluation of GFPs

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RAW 264.7 cells (5 × 10³ cells/well) were cultured in 96-well microplates overnight and treated with serial concentrations (10~160 μg·mL⁻¹) of GFPs for 24 h. Blank control and positive control group were prepared by replacing GFPs solution with equal volume of culture medium and LPS at work concentration of 0.4 μg·mL⁻¹. Then, 10 μL of 5 mg·mL⁻¹ MTT solution in PBS (pH 7.4) was added to each well and incubated for additional 4 h in the dark. After discarding the medium, 100 μL DMSO was added to dissolve the formazan crystals in each well. Finally, the absorbance was detected at 570 nm using a microplate reader (1420 Multilabel counter victor, Perkin-Elmer, Waltham, MA, USA). The percentage of cell viability was expressed as the ratio of absorbance values between samples and blank control groups.

Zhu B., Zhang W., Zhao J., Chen B., Liu F, & Li S. (2022). Characterization and Comparison of Bioactive Polysaccharides from Grifola frondosa by HPSEC-MALLS-RID and Saccharide Mapping Based on HPAEC-PAD. Polymers, 15(1), 208.

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Europium-labeled DELFIA Assay for D-dimer

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The mAb pairs for assay design were selected through a one-step immunoassay. The capture mAbs were adsorbed on plates in phosphate buffer saline (1 μg/100 μl/well; incubation for 30 min). Following three washes with TBST, 75 μl of the detection mAbs in DELFIA assay buffer (200 ng/well) labeled with a stable Eu³⁺ chelate [39 (link)] and 25 μl of the D-dimer-containing sample were added and the mixture was incubated for 1 h. Following six washes with TBST, the enhancement solution (300 μl/well) was added and the mixture was incubated for 3 min under vigorous shaking. The signals were detected using a 1420 Multilabel Counter Victor instrument (Perkin Elmer, USA).

Kogan A.E., Mukharyamova K.S., Bereznikova A.V., Filatov V.L., Koshkina E.V., Bloshchitsyna M.N, & Katrukha A.G. (2015). Monoclonal antibodies with equal specificity to D-dimer and high-molecular-weight fibrin degradation products. Blood Coagulation & Fibrinolysis, 27(5), 542-550.

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Cell Viability Assay with SCH900776

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The cell lines were seeded in 96-well plates in quadruplicates (5 × 10⁴ cells per well, volume 200 μl); half of these cells was treated with 200 nM SCH900776, and after 2 h NAs were applied for 72 h. CLL cells were treated as stated in “Stimulation and treatment of CLL cells”. Four hours before the end of incubation, 10 μl of WST-1 Cell Proliferation Reagent (Roche) was added, and the absorbance was read at 450 nm using spectrophotometer 1420 Multilabel Counter Victor (PerkinElmer).

Zemanova J., Hylse O., Collakova J., Vesely P., Oltova A., Borsky M., Zaprazna K., Kasparkova M., Janovska P., Verner J., Kohoutek J., Dzimkova M., Bryja V., Jaskova Z., Brychtova Y., Paruch K, & Trbusek M. (2016). Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells. Oncotarget, 7(38), 62091-62106.

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Aptamer Binding Affinity Analysis for CFP10

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Example 5

400 pmole of CFP10 was mixed with 10 μL of magnetic beads, and then incubated in 100 μL of a binding buffer (20 mM Tris, 50 mM NaCl, 5 mM KCl, 5 mM MgCl₂, pH 8.0) for 1 hour. To separate unbound CFP10, washing was performed twice with a binding buffer. Subsequently, the resulting mixture was incubated together with various concentrations of a Cy3-labeled aptamer, and an unbound aptamer was removed by washing. The amount of Cy3-labeled ssDNA aptamer bound to the magnetic beads with CFP10 immobilized thereon was measured by fluorescence measurement (1420 Victor Multilabel counter, PerkinElmer, USA) by separating only Cy3-labeled ssDNA bound to CFP10. As a result, as shown in FIG. 3 and Table 2 below, the Kd value of the DNA aptamer having a CG6 base sequence was measured.

TABLE 2

AptamerKd (nM)

CG692.8 ± 26.513

US11028396B2. DNA aptamer specifically binding to CFP10, and use thereof (2021-06-08). MD APTUS INC. [KR]. Inventors: Yoon-Keun Kim [KR], Changill Ban [KR], Chulhun Chang [KR].

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Aptamer Binding Kinetics Characterization

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Example 5

16 μg of TB7.7 was mixed with 10 μL of magnetic beads, and then incubated in 100 μL of a binding buffer (20 mM Tris, 50 mM NaCl, 5 mM KCl, 5 mM MgCl₂, pH 8.0) for 1 hour. To separate unbound TB7.7, washing was performed twice with a binding buffer. Subsequently, the resulting mixture was incubated together with various concentrations of a Cy3-labeled aptamer for an hour, and an unbound aptamer was removed by washing. The amount of Cy3-labeled ssDNA aptamer bound to the magnetic beads with TB7.7 immobilized thereon was measured by fluorescence measurement (1420 Victor Multilabel counter, PerkinElmer, USA) by separating only Cy3-labeled ssDNA bound to TB7.7. As a result, as shown in FIGS. 3A to 3C and Table 2 below, the Kd value of the DNA aptamer having a TG4, TG5, or TG6 base sequence was measured. Through these results, it was confirmed that TG4 was the most stable and exhibited the lowest Kd value, and thus was applied to the subsequent experiments.

TABLE 2

AptamerKd (nM)

TG437.5 ± 2.5

TG5106.3 ± 68.6

TG634.3 ± 7.7

US11634717B2. DNA aptamer specifically binding to TB7.7, and use thereof (2023-04-25). MD APTUS INC. [KR]. Inventors: Yoon-Keun Kim [KR], Changill Ban [KR], Chulhun Chang [KR].

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Quantification of Transformed NIH 3T3 Cells

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NIH 3T3 knockdown cells (5×10³) were seeded using a CytoSelect 96-Well Cell Transformation Assay Kit (Cell Biolabs). For quantification, colonies with >10 cells were counted and normalized to that obtained with a non-silencing shRNA, which was set to 1 (Supplementary Fig. S1B) or cells were lysed, stained with CyQuant and analyzed using a 1420 Victor Multilabel Counter (Perkin Elmer) and a 480/520 nm filter set (Supplementary Fig. S1C). Ponatinib (Selleck) was added to the medium at the concentration(s) indicated in Fig. 5A and B, and soft agar colony formation assays were performed.

Lin L., Chamberlain L., Pak M.L., Nagarajan A., Gupta R., Zhu L.J., Wright C.M., Fong K.M., Wajapeyee N, & Green M.R. (2014). A Large-Scale RNAi-Based Mouse Tumorigenesis Screen Identifies New Lung Cancer Tumor Suppressors that Repress FGFR Signaling. Cancer discovery, 4(10), 1168-1181.

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