Roswell Park Memorial Institute 1640 medium (RPMI 1640) and inactivated fetal bovine serum (BSF) were obtained from Gibco®, Life Technologies (Carlsbad, CA, USA). Prozima Prolav 750 was purchased from Prozyn Biosolutions (São Paulo, SP, Brazil). Ethylenediaminetetraacetic acid (EDTA), l -fucose, d -xylose, d -galactose, d -mannose, d -glucose, d -arabinose, d -rhamnose, d -glucuronic acid, N-d -acetylglucosamine, d -galactosamine, d -glucosamine, and silver nitrate were all obtained from Sigma Aldrich Co. (St. Louis, MO, USA). The other solvents and chemical reagents used in this study were of analytical grade.
D glucosamine
Manufactured by Merck Group
Sourced in United States, Germany
D-glucosamine is a naturally occurring amino sugar that is a key component of glycosaminoglycans, which are structural elements of cartilage and synovial fluid. It is commonly used in the laboratory setting for research and analysis purposes.
Automatically generated - may contain errors
Lab products found in correlation
D mannose, by Merck Group (7 mentions)
D galactose, by Merck Group (6 mentions)
D glucose, by Merck Group (6 mentions)
D glucuronic acid, by Merck Group (5 mentions)
D galactosamine, by Merck Group (5 mentions)
L fucose, by Merck Group (4 mentions)
D xylose, by Merck Group (4 mentions)
D arabinose, by Merck Group (3 mentions)
L rhamnose, by Merck Group (3 mentions)
D rhamnose, by Merck Group (2 mentions)
Silver nitrate, by Merck Group (2 mentions)
N acetylglucosamine, by Merck Group (2 mentions)
Heparin, by Merck Group (2 mentions)
Pullulan standards, by Resonac (2 mentions)
D galacturonic acid, by Merck Group (2 mentions)
L arabinose, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Silica gel 60 f254 aluminum sheet, by Merck Group (2 mentions)
Tryptone, by ITW Reagents (2 mentions)
Yeast extract, by ITW Reagents (2 mentions)
20 protocols using d glucosamine
1
Carbohydrate Characterization of Prozima Prolav 750
2
Virus Propagation in PK15 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PCV2a/LG, PCV2b/LN590516, and PCV2d/SD446F16 (106.0 TCID50/mL) were used as virus seeds to inoculate, respectively, freshly digested PK15 cell suspensions (2 × 105 cells/mL) at a multiplicity of infection of 0.5. Viruses were maintained in MEM containing 2% FBS and 3 mM d-glucosamine (Sigma-Aldrich, St. Louis, MO, USA). After incubation at 37 °C for 120 h, the virus was collected from cultured infected cells by three freeze-thaw cycles and then centrifuged at 2500× g for 10 min at 4 °C. The supernatants containing the virus were aliquoted and stored at -80 °C. Virus titers were measured as described previously [28 (link)]. Samples with virus titers not less than 106.0 TCID50/mL were used for virus purification.
3
Quantification of Chitinase-Released N-Acetylglucosamine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Morgan–Elson assay was used in order to evaluate the N-acetyl-D-glucosamine released after chitinase treatment, as described previously. For more details see [6 (link),11 (link),12 (link)].
Sample preparation for the ESI-MS analysis was performed by the hydrolysis of organic matrixes obtained after HF-treatment of the biological samples in 6M HCl (24 h at 90 °C). The samples, after HCl hydrolysis were filtrated with a 0.4 micron filter and freeze-dried in order to remove any excess HCl. The standard D-glucosamine as a control was purchased from Sigma (Sigma-Aldrich, Taufkirchen, Germany Both the commercial standard and the prepared sample were dissolved in water before ESI-MS analysis. ESI-MS measurements were performed on an Agilent Technologies 6230 TOF LC/MS spectrometer (Applied Biosystems, Foster City, CA, USA) in line as a detector in the analytical HPLC instrument. Nitrogen was used as the nebulizing and desolation gas.
Sample preparation for the ESI-MS analysis was performed by the hydrolysis of organic matrixes obtained after HF-treatment of the biological samples in 6M HCl (24 h at 90 °C). The samples, after HCl hydrolysis were filtrated with a 0.4 micron filter and freeze-dried in order to remove any excess HCl. The standard D-glucosamine as a control was purchased from Sigma (Sigma-Aldrich, Taufkirchen, Germany Both the commercial standard and the prepared sample were dissolved in water before ESI-MS analysis. ESI-MS measurements were performed on an Agilent Technologies 6230 TOF LC/MS spectrometer (Applied Biosystems, Foster City, CA, USA) in line as a detector in the analytical HPLC instrument. Nitrogen was used as the nebulizing and desolation gas.
4
Chitosan-Based Polymer Synthesis and Characterization
5
Anticoagulant Potential of Seaweed M. angicava
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seaweed M. angicava was collected from the coast of Qingdao, China on April 2013, which is in growth mature period of the seaweed. The raw material was thoroughly washed with tap water, air-dried and stored at room temperature in a dry environment. l -rhamnose, l -arabinose, d -xylose, l -fucose, d -mannose, d -galactose, d -glucose, d -glucuronic acid, d -galacturonic acid, d -glucosamine and heparin were from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Pullulan standards (Mw: 21.1, 47.1, 107, 200, 344, and 708 kDa) were from Showa Denko K.K. (Tokyo, Japan). APTT assay reagent (ellagic acid + bovine phospholipids reagent), TT assay reagent (bovine thrombin) and PT assay reagent (rabbit thromboplastin) were from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). PAI-1 and D-dimer kits were from Simens Healthcare Diagnostics Products (Marburg, Germany). FDP kit was from BIOLINKS CO., LTD. (Tokyo, Japan).
6
Fluorescent Iron Oxide Nanoparticle Synthesis
7
Monosaccharide Identification and LPS Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DEAE-cellulose 23 and Sephadex G-150 were purchased from Amersham Biosciences (Uppsala, Sweden). The standard monosaccharide (D-mannose, D-galactose, D-arabinose, D-fructose, L-rhamnose, D-glucuronic acid, D-glucosamine, and D-galactosamine), trifluoroacetic acid (TFA), 1-phenyl-3-methyl-5-pyrazolone (PMP), LPS, and ConA were obtained from Sigma-Aldrich (St. Louis, MO, USA). The RPMI 1640 medium and fetal bovine serum was provided by Gibco (Vienna, NY, USA). All other chemicals and solvents used were of analytical grade and obtained from Sinopharm (Shanghai, China).
8
Synthesis of Metal Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals were of analytical grade and used without purification. Gold(iii ) chloride trihydrate (HAuCl4·3H2O 99.9%), cetyltrimethylammonium bromide (CTAB 99%), d -glucosamine, silver nitrate (AgNO3 99.9%), copper(ii ) chloride (CuCl2), sodium borohydride (NaBH4 99%), l -ascorbic acid (AA 99.8+%), copper(ii ) nitrate hemi-pentahydrate (2Cu(NO3)2·5H2O), and ethanol were obtained from Sigma Aldrich Korea. Sodium hydroxide (98.9+%) and l -glutamic acid were purchased from Dae-Jung Chemical. All of the experiments were conducted in purified water with 18 mQ resistance.
9
Culturing Human Renal Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human renal cancer cell lines 786-O and caki-1 were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (CCCAS, China) and were cultured in RPMI-1640 medium (HyClone) supplemented with 10% fetal bovine serum (Gibco). All cells were cultured in a humidified incubator with 5% CO2 at 37 °C. D-glucosamine was purchased from Sigma Chemical Co (sigma A3286). All the antibodies were purchased from Abcam.
10
Chitin Extraction from Crustacean Waste
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Squid pens, crab shells, and shrimp shells were collected from Shin-Ma Frozen Food Co. (I-Lan, Taiwan). Shrimp head powder (SHP) was gifted from Fwu-Sow Industry (Taichun, Taiwan). Demineralized shrimp shell powder (deSSP) and demineralized crab shell powder (deCSP) were prepared via acid treatment [22 (link)]. Chitin (from shrimp shell), tyrosine, D-glucosamine and the reagents (3,5-dinitrosalicylic acid and Folin-Ciocalteu) were all purchased from Sigma-Aldrich Corp. (Singapore). Macro-prep DEAE and Macro-Prep High S were bought from Bio-Rad (Hercules, CA, USA). All other reagents were the highest grade available. P. macerans TKU029 [16 (link)] was provided by Life Science Development Center, Tamkang University, Taiwan.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!