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Sc 26196

Manufactured by Merck Group
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Sourced in United States

SC-26196 is a laboratory equipment product. It functions as a core component in various experimental and analytical procedures conducted in research and testing environments.

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Lab products found in correlation

Methl pyruvate, by Merck Group (2 mentions) Inverted infinity and phase contrast microscope, by Thermo Fisher Scientific (2 mentions) Methocult m3434, by STEMCELL (2 mentions) Mts cell proliferation colorimetric assay kit, by Abcam (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

4 protocols using sc 26196

1

Cell Proliferation Assay for Radiation and Drug

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Cell proliferation after treatments of SC-26196 (Sigma-Aldrich Co., St Louis, MO, USA), radiation, or the combination of both was assessed using an MTS Cell Proliferation Colorimetric Assay Kit (Abcam, Cambridge, MA, USA). Briefly, U-87 MG and LN-229 cells were seeded at 5,000 cells per well into 96-well plates and incubated overnight. Then, the cells were exposed to different treatments including SC-26196 alone, X-ray irradiation alone, and the combination of both at various doses. After a 48-hour incubation, 20 µL/well of MTS reagent was added, and the absorbance was measured using a plate reader at 490 nm within 4 hours of incubation. All experiments were repeated at least three times.
Wang J., Liang H., Sun M., Zhang L., Xu H., Liu W., Li Y., Zhou Y., Li Y, & Li M. (2018). Delta-6-desaturase inhibitor enhances radiation therapy in glioblastoma in vitro and in vivo. Cancer Management and Research, 10, 6779-6790.
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2

Modulation of Lipid Metabolism in HepG2 Cells

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HepG2 liver cells were grown in DMEM (Life Technologies) with 10% fetal bovine serum (Life Technologies) and 100 units/mL penicillin, 100 μg/mL streptomycin (Life Technologies) for 2 days. Cells were then treated with vehicle (0.5% ethanol in distilled deionized water), DPA (Nu-Chek-Prep, Elysian, MN) (50μM), or DPA+ CUR (Sigma-Aldrich, Saint Louis, MO) (10, 20, or 40μM) for 48 hours. A separate set of cells were treated with FADS2 (Δ6-desaturase) inhibitor SC-26196 (Sigma-Aldrich, Saint Louis, MO) (2μM) 30min before treatment with DPA (50μM) + Cur (20μM). At the end of each experiment, the cells were harvested for immunoblotting or lipid analysis.
Wu A., Noble E.E., Tyagi E., Ying Z., Zhuang Y, & Gomez-Pinilla F. (2014). Curcumin boosts DHA in the brain: implications for the prevention of anxiety disorders. Biochimica et biophysica acta, 1852(5), 951-961.
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3

Hematopoietic Stem Cell Clonogenic Assay

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Sorted mouse HSC (180 cells Lin−/cKit+/Sca1+/CD48−/CD150+) or CD34-enriched human HSPC (5,000 cells) were plated into methylcellulose media (STEMCELL Technologies, MethoCult M 3434 for mouse or MethoCult H4434 for human) according to the manufacturer’s protocols. Colonies were scored after 10 to 12 days for mouse and 14 to 16 days for human using an Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). For serial re-plating assays, methylcellulose medium from primary platings were dissolved in PBS to dissociate the colonies into a single-cell suspension, washed three times and 20,000 cells (mouse) or 40,000 cells (human) were re-plated in 1ml of MethoCult M3434 or MethoCult H4434 medium for mouse and human cells, respectively. Where indicated, media was supplemented with: 100 nM FADS2 inhibitor SC-26196 (Sigma, PZ0176), 100 μM γ-linolenic fatty acid (GLA) (Sigma, L2378), 100 μM NAC (Sigma, A7250) or 5 mM Methl-pyruvate (Sigma, 371173).
Dong S., Wang Q., Kao Y., Diaz A., Tasset I., Kaushik S., Thiruthuvanathan V., Zintiridou A., Nieves E., Dzieciatkowska M., Reisz J., Gavathiotis E., D’Alessandro A., Will B, & Cuervo A. (2021). Chaperone-mediated autophagy sustains hematopoietic stem cell function. Nature, 591(7848), 117-123.
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4

Hematopoietic Stem Cell Clonogenic Assay

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Sorted mouse HSC (180 cells Lin−/cKit+/Sca1+/CD48−/CD150+) or CD34-enriched human HSPC (5,000 cells) were plated into methylcellulose media (STEMCELL Technologies, MethoCult M 3434 for mouse or MethoCult H4434 for human) according to the manufacturer’s protocols. Colonies were scored after 10 to 12 days for mouse and 14 to 16 days for human using an Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). For serial re-plating assays, methylcellulose medium from primary platings were dissolved in PBS to dissociate the colonies into a single-cell suspension, washed three times and 20,000 cells (mouse) or 40,000 cells (human) were re-plated in 1ml of MethoCult M3434 or MethoCult H4434 medium for mouse and human cells, respectively. Where indicated, media was supplemented with: 100 nM FADS2 inhibitor SC-26196 (Sigma, PZ0176), 100 μM γ-linolenic fatty acid (GLA) (Sigma, L2378), 100 μM NAC (Sigma, A7250) or 5 mM Methl-pyruvate (Sigma, 371173).
Dong S., Wang Q., Kao Y., Diaz A., Tasset I., Kaushik S., Thiruthuvanathan V., Zintiridou A., Nieves E., Dzieciatkowska M., Reisz J., Gavathiotis E., D’Alessandro A., Will B, & Cuervo A. (2021). Chaperone-mediated autophagy sustains hematopoietic stem cell function. Nature, 591(7848), 117-123.
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