Acetyl coenzyme a acetyl coa sodium salt

GNE1, N²-(((1r,4r)-4-aminocyclohexyl)methyl)-5-chloro-N⁴-(5-cyclopropyl-1H-pyrazol-3-yl)pyrimidine-2,4-diamine; GNE2, N²-(((1r,4r)-4-aminocyclohexyl)methyl)-N⁴-(5-cyclo-
propyl-1H-pyrazol-3-yl)pyrimidine-2,4-diamine; GNE3, 2-(4-aminopiperidin-1-yl)-N-(5-cyclopropyl-1H-pyrazol-3-yl)
thieno [3 (link),2-d]pyrimidin-4-amine; and the N-acetylated metabolite of GNE1 (GNE4), N-((1r,4r)-4-(((5-chloro-4-((5-cyclopropyl-1H-pyrazol-3-yl)amino)pyrimidin-2-yl)amino)
methyl)cyclohexyl)acetamide were synthesized in-house (supplemental method). Acetyl coenzyme A (acetyl-CoA) sodium salt, 1-aminobenzotriazole (ABT), bovine serum albumin, ethylenediaminetetraacetic acid (EDTA), indomethacin, ketamine/xylazine HCl solution, Krebs-Henseleit buffer, labetalol, loperamide, and polyethylene glycol (PEG400) were purchased from Sigma-Aldrich (St. Louis, MO). Tetrasodium salt of NADPH was obtained from EMD Millipore (Billerica, MA). All other reagents and solvents of highest quality were purchased from commercial vendors.

Diethylstilbestrol (DES, CAS 56-53-1), 17α-ethinylestradiol (EE2, CAS 57-63-6), genistein (GEN, CAS 446-72-0), coumestrol (COU, CAS 479-13-0), methoxychlor (MXC, CAS 72-43-5), reduced nicotinamide adenine dinucleotide phosphate (NADPH), uridine 5ʹ-diphosphoglucurnic acid (UDPGA), adenosine 3ʹ-phosphate 5ʹ-phosphosulfate (PAPS), lithium salt hydrate, acetyl coenzyme A (acetyl CoA) sodium salt, alamethicin, magnesium chloride, sodium phosphate, sodium chloride, and rat serum were purchased from Sigma-Aldrich (Zwijndrecht, the Netherlands). Dimethyl sulfoxide (DMSO) was purchased from Acros Organics (Geel, Belgium) and phosphate-buffered saline was purchased from Invitrogen (Breda, the Netherlands). Pooled liver S9 fractions from male and female Sprague-Dawley rats were obtained from Tebu-bio (Heerhugowaard, the Netherlands). Rapid equilibrium dialysis (RED) devices, including RED inserts, RED base plate and sealing tape, were purchased from Fisher (Landsmeer, the Netherlands).