E1015

Manufactured by Applygen

Sourced in China

E1015 is a compact and versatile laboratory centrifuge designed for a wide range of applications. It features a maximum speed of 15,000 rpm and can accommodate a variety of sample sizes and tube types. The centrifuge is equipped with a user-friendly digital display and intuitive controls for easy operation.

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Lab products found in correlation

E1013, by Applygen (15 mentions) E1005, by Applygen (3 mentions) E1016, by Applygen (3 mentions) Gpo pod, by Applygen (2 mentions) Enhanced bca protein quantitation assay kit, by Keygen Biotech (1 mentions) E1003, by Applygen (1 mentions) 7600 autoanalyzer, by Hitachi (1 mentions) Bicinchoninic acid protein assay kit, by Applygen (1 mentions) G7021, by Merck Group (1 mentions) Enzyme linked immunosorbent assay kit, by Nanjing Jiancheng (1 mentions) Multimode microplate reader, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Tecan (1 mentions) Dounce homogenizer, by Thermo Fisher Scientific (1 mentions) P1511, by Applygen (1 mentions) Ab207612, by Abcam (1 mentions) Ab32028, by Abcam (1 mentions) Ab8805, by Abcam (1 mentions) Ab182651, by Abcam (1 mentions) Ab48394, by Abcam (1 mentions) Ab192890, by Abcam (1 mentions)

Spelling variants of this product

Tissue total cholesterol assay kit E1015 (3 citations) E1015-105 (3 citations) Cholesterol assay kit (E1015) (2 citations)

29 protocols using e1015

Serum and Hepatic Lipid Profile Analysis

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Serum concentration of total cholesterol (Tch) was measured with a biochemical automatic analyzer (Hitachi 7020; HITACHI) using a commercial cholesterol assay kit (E1015; Applygen Technologies, Inc.). Serum concentrations of LDL-cholesterol (LDL-C) and HDL-cholesterol were measured with a biochemical automatic analyzer (Hitachi 7020; HITACHI) using a commercial cholesterol assay kit (KP712, KF253 Wako Pure Chemical Industries, Ltd. Wako), respectively. Serum concentration of TG was measured with a biochemical automatic analyzer (Hitachi 7020; HITACHI) using a commercial TG assay kit (E1013; Applygen Technologies, Inc.). Serum non-esterified fatty acid (NEFA) concentrations were determined using the Wako NEFA Cacyllcoenzyme A synthetase acyl-coenzyme A oxidase assay method. Hepatic total cholesterol concentration was measured using a tissue total cholesterol assay kit (E1015; Applygen Technologies, Inc.) following the manufacturer’s instructions. Hepatic concentration of total triglyceride was quantified with a biochemical automatic analyzer (Hitachi 7020; HITACHI) using a commercial TG assay kit (E1013; Applygen Technologies, Inc.).

Zhou J., Gao S., Chen J., Zhao R, & Yang X. (2016). Maternal sodium butyrate supplement elevates the lipolysis in adipose tissue and leads to lipid accumulation in offspring liver of weaning-age rats. Lipids in Health and Disease, 15, 119.

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Hepatic Triglyceride and Cholesterol Assay

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Hepatic triglyceride (TG) and Chol were determined by using a commercial assay kit (E1013 and E1015, Applygen Technologies Inc., China) following the manufacturer's instructions.

Wu L., Liu X., Zhang A., Chen H., Zhao R, & Jia Y. (2023). Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken. Frontiers in Veterinary Science, 10, 1147024.

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Lipid Profile Analysis of Biological Samples

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Bile, homogenized liver and feces were used to extracted lipids using FOLCH (chloroform:methonal-2:1) as described previously [18 (link)] and enzymatically measured using commercially available kits (Cholesterol: E1015, Applygen, China; Triglyceride: E1013, Applygen, China; Bile acids: BI7710, Zhicheng Biological Technology, China; Phospholipid: EFR0178, FUJIFILM, Japan). All procedures were carried out in accordance to the instructions by kits.

Shen W., Shao W., Wang Q., Wang B., Zhao G., Gu A., Jiang Z, & Hu H. (2023). Dietary diosgenin transcriptionally down-regulated intestinal NPC1L1 expression to prevent cholesterol gallstone formation in mice. Journal of Biomedical Science, 30, 44.

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Macrophage Cholesterol Metabolism Regulation

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Macrophages were transfected with shRNA-NC, shRNA-PAR2-1 for 48 h and treated with ox-LDL (100 μg/ml) for another 24 h. Further, macrophages co-transfected with shRNA-PAR2 and pcDNA3.0-DKK1 for 48 h and treated with ox-LDL (100 μg/ml) for another 24 h. The total cholesterol and free cholesterol levels were detected using the commercial kit (E1015, E1016; Applygen Technologies Inc., Beijing, China) following the instructions. Cells were treated with lysis buffer for 10 min and centrifuged at 1500 × g for 10 min. The supernatant was collected and detected at 550 nm.

Liu Y., Wei M., Liu G., Song C., Yang M., Cao Z, & Zheng M. (2020). Silencing Protease-Activated Receptor-2 alleviates ox-LDL-induced lipid accumulation, inflammation and apoptosis via activation of Wnt/β-catenin signaling. General physiology and biophysics, 39(5).

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Liver Lipid Quantification Assays

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The triglyceride content and total cholesterol in the liver were assayed using a triglyceride assay kit (E1015, Applygen Technologies, Beijing, China) and a total cholesterol assay kit (GPO-POD; Applygen Technologies, Beijing, China), respectively, according to the manufacturer’s recommended protocol.

Zhou L., Ding S., Li Y., Wang L., Chen W., Bo T., Wu K., Li C., Liu X., Zhao J., Xu C, & Gao L. (2016). Endoplasmic Reticulum Stress May Play a Pivotal Role in Lipid Metabolic Disorders in a Novel Mouse Model of Subclinical Hypothyroidism. Scientific Reports, 6, 31381.

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Tissue Triglyceride and Cholesterol Quantification

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TG and cholesterol was detected by the Tissue/Cell Triglyceride (TG) Content Enzymatic Determination Kit and Enzymatic assay kit to determine the total cholesterol (CHOL) in tissue cells (E1013, E1015, Applygen Technologies Inc., Beijing, China) according to the manufacturer’s instructions. Adipose tissue was added into lysate, grinded by electric homogenizer and left on ice for 10 min. A total of 10 µL of the liquid was removed for protein concentration assay correction. The remaining liquid was heated at 70 °C for 10 min. The upper clarified liquid was centrifuged at 2000 rpm for 5 min at room temperature and removed for enzymatic assay. The optical density of each sample was determined using a multi-function microplate reader (Biotech, San Francisco, CA, USA). The experiment was repeated three times and three technical replicates were performed for each sample. The total protein concentration was measured with the Enhanced BCA Protein Quantitation Assay Kit (KGP902, KeyGEN BioTECH, Nanjing, China). The TG content was finally corrected per mg protein content.

Lu X., Fang X., Mi J., Liu Y., Liu R., Li G., Li Y, & Yang R. (2023). Effects of Adipose Tissue-Specific Knockout of Delta-like Non-Canonical Notch Ligand 1 on Lipid Metabolism in Mice. International Journal of Molecular Sciences, 25(1), 132.

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Quantifying Cellular Lipid Profiles

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TAG and cholesterol contents in GMEC were quantified using a triacylglycerol enzymatic assay kit (E1013, Applypen Technologies Inc., Beijing, China) and cholesterol enzymatic assay kit (E1015, Applygen Technologies Inc., Beijing, China) following the manufacturer’s instructions. Cellular TAG and cholesterol content is shown as the ratio of TAG or cholesterol concentration to the protein concentration. Detailed procedures for these assays have been reported previously [33 (link)].

Huang L., Luo J., Gao W., Song N., Tian H., Zhu L., Jiang Q, & Loor J.J. (2022). CRISPR/Cas9-Induced Knockout of miR-24 Reduces Cholesterol and Monounsaturated Fatty Acid Content in Primary Goat Mammary Epithelial Cells. Foods, 11(14), 2012.

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Quantifying Cholesterol and Triglycerides in Cells and Tissues

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The levels of cholesterol and triglyceride in cellular or tumor tissues (from nude mice) were assessed using total cholesterol assay kit E1015 (Applygen Technologies Inc., Beijing, China) and triglyceride assay kit E1013 (Applygen Technologies Inc.). All experiments were performed in accordance with the manufacturer’s instructions. In detail, 0.1 mL of lysis buffer was added to 1 × 10⁶ cells, and then 10 µL lysis buffer was added to each 1 mg of tissue.

Jin Y., Chen Z., Dong J., Wang B., Fan S., Yang X, & Cui M. (2021). SREBP1/FASN/cholesterol axis facilitates radioresistance in colorectal cancer. FEBS Open Bio, 11(5), 1343-1352.

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Comprehensive Lipid Profiling Protocol

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The content of total cholesterol and triglycerides (TG) in serum was measured using commercial assay kit (E1005 and E1003 respectively; Applygen Technologies, Inc. Beijing, China). Concentrations of LDL cholesterol (LDLC) and high-density lipoprotein cholesterol (HDLC) in serum were measured with respective assay kits (006340 and 006328, Beijing BHKT Clinical Reagent Co., Ltd., Beijing, China). The cholesterol and TG in liver was determined according to the instruction of a tissue commercial assay kit (E1015 and E1013 respectively; Applygen Technologies, Inc. Beijing, China). Hepatic and serum bile acid content was detected by enzymatic colorimetric methods according to a commercial bile acid assay kit (E003; Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China).

Cai D., Yuan M., Liu H., Pan S., Ma W., Hong J, & Zhao R. (2016). Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification. Nutrients, 8(10), 646.

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Hepatic Cholesterol Quantification

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Hepatic total cholesterol and free cholesterol concentration were measured using a tissue total cholesterol assay kit (E1015; Applygen Technologies, Inc.) and free cholesterol assay kit (E1016; Applygen Technologies, Inc.) following the manufacturer's instructions. Cholesteryl ester was quantified by subtracting the free cholesterol values from the total cholesterol value.

Wen G., Yao L., Hao Y., Wang J, & Liu J. (2022). Bilirubin ameliorates murine atherosclerosis through inhibiting cholesterol synthesis and reshaping the immune system. Journal of Translational Medicine, 20, 1.

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