Cd11c cre mice

Mice were bred and housed under SPF conditions in the vivarium of the La Jolla Institute for Allergy & Immunology (LJI, La Jolla, CA). Female 8 week old Il10^−/−, Il10rb^−/− and Rag^−/− were purchased from Jackson laboratory. Female 8 week old Il10^gfp (VertX) were obtained from Christopher Karp (Cincinnati Children’s Hospital, OH) and also purchased from Jackson Laboratory. Il10^−/−Rag^−/− mice were described previously^{47 (link)}. Il10^flox/flox mice were obtained from Alexander Rudensky (Memorial Sloan-Kettering Cancer Center, NY) with the permission of Axel Roers (Dresden University of Technology, Germany). Il10^flox/flox mice were bred to Cd4-Cre, LysM-Cre and Cd11c-Cre mice, which were all purchased from Jackson laboratory. All mouse strains were generated on the C57BL6/J background or backcrossed at least 10 generations. All procedures were approved by the LJI Animal Care and Use Committee.

All procedures and protocols carried out during this study were approved under The Institutional Animal Care and Use Committee (IACUC) at the University of Minnesota. Female BALB/c (H-2^d), C57BL/6 (B6; H-2^b) mice were purchased from Charles River Laboratories. Female B10.BR (H-2^k), B6.Cg-Tg (Vil1-cre) (B6 villin^Cre) and CD11c^Cre mice were purchased from The Jackson Laboratory. In villin^Cre mice, the expression of Cre recombinase is restricted to villus and crypt epithelial cells of the small and large intestines. B6 CYP26A1 flox/flox (loxP-STOP-loxP-CyP26A1) mice were generated by two of the authors (YCL and RNJ, Dartmouth College, USA). B6 RALDH1 flox/flox frozen embryos and RALDH2 flox/flox mice were kindly provided by Drs. Pierre Chambon and Norbert Ghyselinck (Institute for Genetics and Cellular and Molecular Biology, France). RALDH1 flox/flox embryos were implanted into pseudopregnant mothers to generate RALDH1 flox/flox offspring. Both female and male B6 CYP26A1^stop/stop, B6 RALDH1 flox/flox, RALDH2 flox/flox and conditional strains of each were used in experiments. Mice were housed in a specific-pathogen free facility in micro-isolator cages maintained by the University of Minnesota.

CD11c.Cre mice and OT-II mice were purchased from Jackson Laboratories. FOXO1^L/L mice were generously provided by Dr. Ronald DePinho (University of Texas MD Anderson Cancer Center, Houston, Texas) (18 (link)). FOXO1^L/L mice were bred with CD11cCre mice to generate FOXO1 deleted mice (CD11c.Cre⁺.FOXO1^L/L) and the control littermates (CD11c.Cre⁻.FOXO1^L/L) (19 ). All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Pennsylvania.
Mice were challenged by intraperitoneal injection of lightly fixed P. gingivalis (ATCC, #33277) (10⁹ CFU once weekly) or sham with vehicle alone (PBS) and euthanized one week after the last injection or oral inoculated by P. gingivalis/F.nucleatum (ATCC, #25586) as previously described (20 (link)) three times weekly for two weeks. Mice were euthanized 6 weeks after the last oral inoculation. Antibody (IgG1 or IgG2a) against P. gingivalis was measured by ELISA as previously described and the concentration was determined by reference to a standard curve (20 (link)).