Pyrosequencing of the amplicons was performed according to the manufacturer's protocol using the GS Junior System (Roche Diagnostics). Emulsion PCR, breaking, and bead enrichment were conducted using the GS Junior Titanium emPCR Kit, Lib-L emPCR Reagents, Lib-L Kit, Oil and Breaking Kit, and the Bead Recovery Reagents Kit according to the supplier's instructions (Roche Diagnostics). For emulsion PCR, we used a copy-per-bead ratio of 0.5. Enrichment of the DNA-carrying magnetic beads was accomplished using a magnetic particle collector (Invitrogen, Life Technologies). The quantity of the enriched beads was determined with the GS Junior Bead Counter (Roche Diagnostics). Finally, we loaded 100,000 to 500,000 beads onto the PicoTiterPlate (Roche Diagnostics). Sequencing was carried out according to standard Roche/454 protocols using the GS Titanium Sequencing Kit (Roche Diagnostics) and the GS Junior device.
Magnetic particle collector
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Magnetic Particle Collector is a laboratory equipment designed to efficiently separate and collect magnetic particles from a liquid suspension. It utilizes a strong magnetic field to attract and concentrate the magnetic particles, allowing for easy removal and further processing or analysis.
Automatically generated - may contain errors
Lab products found in correlation
Gs junior bead counter, by Roche (2 mentions)
Picotiterplate, by Roche (2 mentions)
Gs titanium sequencing kit, by Roche (2 mentions)
Gs junior device, by Roche (2 mentions)
Gs junior system, by Roche (2 mentions)
Oil and breaking kit, by Roche (2 mentions)
Bead recovery reagents kit, by Roche (2 mentions)
Lib l kit, by Roche (1 mentions)
Gs junior titanium empcr kit, by Roche (1 mentions)
Gs junior titanium empcr kit lib l, by Roche (1 mentions)
2 protocols using magnetic particle collector
1
Pyrosequencing of Amplicons using GS Junior
2
Pyrosequencing with GS Junior System
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pyrosequencing was carried out according to the manufacturer's protocol for amplicons using the GS Junior System (Roche Diagnostics). Emulsion PCR, breaking, and bead enrichment was carried out using the GS Junior Titanium emPCR Kit Lib-L, emPCR Reagents Lib-L kit, Oil and Breaking kit, and the Bead Recovery Reagents kit according to the supplier's instructions (Roche Diagnostics). For emulsion PCR, we used a copy-per-bead ratio of 0.5. Enrichment of DNA-carrying beads was done with magnetic beads and a magnetic particle collector (Invitrogen, Carlsbad, CA, USA). To evaluate the amount of enriched beads, counting was performed using the GS Junior Bead Counter (Roche Diagnostics). Finally, we loaded 100,000-500,000 beads onto the PicoTiterPlate (Roche Diagnostics). Sequencing was carried out according to standard Roche/454 protocols using the GS Titanium Sequencing kit (Roche Diagnostics) and the GS Junior device.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!