The A375 and Malme-3M melanoma cell lines lines used in this study were purchased from the ATCC. The WM983B melanoma cell line was a gift from L. Larue (Institut Curie, France). The A375 and WM983B cell lines were maintained at 37 °C and 5% CO2 in a humidified atmosphere and grown in Dulbecco’s modified Eagle’s medium (DMEM) growth media supplemented with 10% fetal bovine serum (FBS) and 2 mM glutamine (Gibco). The Malme-3M cells were grown in Iscove’s modified Dulbecco’s medium supplemented with 20% FBS. The PC9 lung cancer cell line was grown in RPMI1640 supplemented with 10% FBS. All cell lines were regularly controlled to be mycoplasma-free by using a PCR-based test (Biovalley). BRAF inhibitor (PLX4032, #S1267), MEKi (Cobimetinib, #S8041), EGFR inhibitor (Erlotinib, #S1023), KDM6B inhibitor (GSK-J1, #S7581) and CBP/p300 inhibitor (SGC-CBP30, #S7256) were purchased from Selleckchem (Euromedex, France). Silvestrol was purchased from MedChem Tronica (# HY-13251, Sweden). Pateamine A was provided by S. Apcher (Gustave Roussy Institute, France). Hippuristanol was provided by J. Tanaka (University of the Ryukyus, Japan). 4E1RCat (#SML0197) and cycloheximide (CHX, #C104450) was purchased from Sigma. All the chemicals were dissolved in dimethylsulfoxide (DMSO) for in vitro studies. The panel of small-molecule chemical library was obtained from L. Désaubry (Strasbourg, France).
Sgc cbp30
Manufactured by Selleck Chemicals
Sourced in United States
The SGC-CBP30 is a laboratory equipment product designed for use in chemical and scientific research applications. It serves as a core function tool without further interpretation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Bortezomib, by Selleck Chemicals (2 mentions)
Plx4032, by Selleck Chemicals (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Wm983b, by Thermo Fisher Scientific (1 mentions)
Dmem growth media, by Thermo Fisher Scientific (1 mentions)
Gsk j1, by Selleck Chemicals (1 mentions)
4e1rcat, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Jib 04, by Selleck Chemicals (1 mentions)
Trametinib, by Selleck Chemicals (1 mentions)
I bet151, by Selleck Chemicals (1 mentions)
Penicillin, by Selleck Chemicals (1 mentions)
Arpe 19, by American Type Culture Collection (1 mentions)
Mum 2b, by Cell Resource Center (1 mentions)
Solcitinib, by Selleck Chemicals (1 mentions)
Ulixertinib, by Selleck Chemicals (1 mentions)
Pd166866, by Selleck Chemicals (1 mentions)
Fgf 1, by Thermo Fisher Scientific (1 mentions)
10 protocols using sgc cbp30
1
Melanoma and Lung Cancer Cell Lines
2
Pharmacological Agents for Cell Signaling
3
Evaluating Histone Acetyltransferase Inhibitors
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A human retinal pigment epithelium cell line (ARPE-19) was obtained from ATCC (ATCC CRL2302, Manassas, VA). Human UM cell lines (MuM-2B and C918) were obtained from the Cell Resource Center, Peking Union Medical College (Beijing, China). All cell lines were cultured in RPMI-1640 medium with supplementation of 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA) and appropriate amounts of penicillin (100 U/mL) and streptomycin (100 mg/mL) in a humidified atmosphere of 5% CO2 at 37° C. The histone acetyltransferase inhibitors C646, which targets EP300, and SGC-CBP30, which targets cAMP response element binding protein (CREB) binding protein (CREBBP), were purchased from Selleck (Shanghai, China).
4
Targeting Oncogenic Signaling Pathways in Breast Cancer
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The drugs listed below were acquired from Selleck chem: FGFR inhibitors PD166866 (specific to FGFR1), Alofanib (targeting FGFR2), H3B-6527 (inhibiting FGFR4), and AZD4547 (effective against FGFR1-3), pan-FGFR inhibitor TAS-120, JAK inhibitors Solcitinib (inhibiting JAK1) and AZD1480 (inhibiting JAK2), UC2288 (p21 inhibitor), STAT inhibitors Fludarabine (inhibiting STAT1),
Stattic (inhibiting STAT3) and BAY2353 (Niclosamide, inhibiting STAT3), SGC-CBP30 (potent CREBBP/EP300 inhibitor), and ulixertinib (ERK1/ERK2 inhibitor). Human EGF protein and FGF ligands including FGF1, FGF2, FGF4, FGF7, FGF9, FGF8a, FG19 and FGF21 were purchased from PeproTech. The breast cancer cell lines CAMA1, MDA-MB-134, MCF7, and T47D were obtained from the American Type Culture Collection (ATCC). The CAMA1 and MCF7 cell lines were grown in DMEM with a 10% FBS and 1% antibiotic-antimycotic solution, while T47D and MDA-MB-134 were cultured in RPMI with 10% FBS and 1% antibiotic-antimycotic solution. Regular testing for mycoplasma contamination was conducted using the commercially available Myco Alert kit from Lonza. All cell lines utilized in this research have undergone authentication by ATCC, and only cells with a low number of passages were employed in experiments to ensure work confidence.
Stattic (inhibiting STAT3) and BAY2353 (Niclosamide, inhibiting STAT3), SGC-CBP30 (potent CREBBP/EP300 inhibitor), and ulixertinib (ERK1/ERK2 inhibitor). Human EGF protein and FGF ligands including FGF1, FGF2, FGF4, FGF7, FGF9, FGF8a, FG19 and FGF21 were purchased from PeproTech. The breast cancer cell lines CAMA1, MDA-MB-134, MCF7, and T47D were obtained from the American Type Culture Collection (ATCC). The CAMA1 and MCF7 cell lines were grown in DMEM with a 10% FBS and 1% antibiotic-antimycotic solution, while T47D and MDA-MB-134 were cultured in RPMI with 10% FBS and 1% antibiotic-antimycotic solution. Regular testing for mycoplasma contamination was conducted using the commercially available Myco Alert kit from Lonza. All cell lines utilized in this research have undergone authentication by ATCC, and only cells with a low number of passages were employed in experiments to ensure work confidence.
5
Cardiac Progenitor Cell Manipulation
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Cardiac progenitor cells were kindly provided by the Molecular Oncology Laboratory of the University of Chicago Medical Center (Illinois, USA)15 (link) and cultured in DMEM/F12 (Gibco, China) containing 10% fetal bovine serum (Gibco, Australia) and penicillin/streptomycin. The cells were incubated at 37 °C in a humidified atmosphere of 5% CO2. Lentivirus containing three target P300 siRNAs were obtained from HANBIO (Shanghai, China). The target sequence was as follows: 5′- GGAACTACCCTAC TTTGAAGG -3′. Transfection was performed according to the manufacturer's instructions. In the inhibitor experiment, SGC-CBP30 (Selleck, Shanghai, China) was added at a concentration of 2 μM for 6 h prior to RT-qPCR and Chromatin immunoprecipitation.
6
Targeting Epigenetic Regulators in Cancer
7
Investigating CTLA4 and IRF1 Regulation
8
Compound Treatment on BCLs
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BCLs were continuously treated for 72 h in adherent conditions with bortezomib (stock concentration SC = [10 mM], Selleckchem), carfilzomib (SC = [10 mM], Selleckchem), chloroquine (SC = [10 mM], Selleckchem), Cortistatin A (SC = [100 μM], a kind gift from Prof. P. Baran, The Scripps Research Institute, La Jolla, CA, USA), docetaxel (SC = [10 mM], GSK343 (SC = [1 mM], Active Biochemicals Co), I‐CBP112 (SC = [10 mM], Sigma), JQ1 (SC = [10 mM], Selleckchem), mifepristone (SC = [10 mM], Selleckchem), Ro5‐3335 (SC = [10 mM], Calbiochem), ruxolitinib (SC = [10 mM], Selleckchem), SGC‐CBP30 (SC = [10 mM], Selleckchem), salinomycin (SC = [200 μM], Selleckchem), spliceostatin A (SC = [10 mM], Adooq Biosciences), tazemetostat (SC = [5 mM], Selleckchem), and 8‐AZA (SC = [100 mM], Chembiotech). All these compounds were resuspended in dimethyl sulfoxide (DMSO; Sigma), except for chloroquine resuspended in H2O. For the in vivo experiments, salinomycin (SC = [6 mg/ml], Medchemexpress) and JQ1 (SC = [100 mg/ml], Medchemexpress) were resuspended in a solution of DMSO/(2‐Hydroxypropyl)‐β‐cyclodextrin (HPCD) 10% (1:9, v/v).
9
Modulating Cholangiocyte Responses to LPS
10
Silkworm Embryo Cell Line Treatments
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The BmE silkworm embryo cell line was maintained in Grace’s insect cell culture medium (Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Waltham, MA, USA) at 27 °C. Cells were incubated with 2.5 μM ecdysone (H5142; Sigma-Aldrich, St Louis, MO, USA) or 2 μM trichostatin A (S1045; Selleck Chemicals, Houston, TX, USA) in culture medium for 12 h, unless otherwise stated. BmE cells were treated with 30 μM C646 (S7152; Selleck Chemicals, Houston, TX, USA) or 25 μM SGC-CBP30 (S7256; Selleck Chemicals, Houston, TX, USA) for 30 min before harvesting. As a vehicle control, the cells were treated with DMSO in parallel.
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