2.4g2 mab

Manufactured by Thermo Fisher Scientific

The 2.4G2 mAb is a monoclonal antibody that binds to the Fc gamma receptor II (FcγRII) on mouse cells. It is commonly used as a blocking reagent to prevent Fc receptor-mediated binding of antibodies or other Fc-containing proteins in various immunological applications.

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Lab products found in correlation

Cd8 fitc, by BioLegend (1 mentions) Cd11b fitc, by BioLegend (1 mentions) Cd19 fitc, by BioLegend (1 mentions) Cd11c fitc, by BD (1 mentions) Lung dissociation kit, by Miltenyi Biotec (1 mentions) Fortessa, by BD (1 mentions) Fixable aqua dead cell staining kit, by Thermo Fisher Scientific (1 mentions) Cd3 fitc 145 2c11, by Thermo Fisher Scientific (1 mentions) Cd4 fitc gk1, by Thermo Fisher Scientific (1 mentions) Nk1.1 fitc, by BioLegend (1 mentions) Gr1 fitc rb6 8c5, by BioLegend (1 mentions) Cd11c hl3, by BD (1 mentions) Rbc lysing buffer, by Merck Group (1 mentions) Gentlemacs, by Miltenyi Biotec (1 mentions) Cd11b m1 70, by BD (1 mentions) Siglec f, by BD (1 mentions) Anti cd4 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions) Foxp3 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti h2b pe, by BD (1 mentions)

Close spelling variants of this product

Monoclonal antibody (mAb) against CD16/CD32 (clone 2.4G2, (2 citations)

2 protocols using 2.4g2 mab

Comprehensive Lung Immune Cell Profiling

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BAL and lung cells were treated with RBC lysing buffer (Sigma). Lungs were dissociated using a Lung Dissociation Kit (Miltenyi Biotec) and Gentle MACS (Miltenyi Biotec). LIVE/DEAD cells were stained with Fixable Aqua Dead Cell Staining Kit (Thermo Fisher Scientific), and after Fc block with the 2.4G2 mAb (eBioscience), cells were stained with the following Abs: DR3-PE (4C12; BioLegend), SiglecF (E50-2440; BD Biosciences), CD11b (M1/70; BD Biosciences), CD11c (HL3; BD Biosciences), Ly6G (1A8; BioLegend), ST2-PE (101001PE, MD), CD90.2 (30-H12; BioLegend). For lineage markers for ILC2 staining, the following Abs were used: CD3-FITC (145-2C11; eBioscience), CD4-FITC (GK1.5; eBioscience), CD8- FITC (5H-10-1; BioLegend), CD19-FITC (1D3; BioLegend), NK1.1-FITC (PK136; BioLegend), CD11b-FITC (M1/70; BioLegend), CD11c-FITC (HL3; BD Biosciences), and Gr1-FITC (RB6-8C5; BioLegend). Flow cytometry analysis was performed on a Fortessa (BD Biosciences), and data were analyzed using FlowJo Software (version 10; FlowJo, Ashland, OR). Live CD45+ lung immune cells were separated into T cells (CD3+, CD90.2+), macrophages (CD11b+, CD11c+ SiglecF+), DCs (CD11c+ MHC class II+), neutrophils (GR1+, CD11b+ SigF-), eosinophils (Ly6C+, SigF+, CD11c-) and ILC2 (Lin^- Thy1.2⁺ ST2⁺Sca1⁺).

Steele H., Sachen K., McKnight A.J., Soloff R, & Herro R. (2021). Targeting TL1A/DR3 Signaling Offers a Therapeutic Advantage to Neutralizing IL13/IL4Rα in Muco-Secretory Fibrotic Disorders. Frontiers in Immunology, 12, 692127.

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Analysis of Th Cells in Helminth Infection

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In experiments that did not involve BMT, spleen and MLN cells from uninfected and Hpb-infected BALB/c WT, STAT6^–/–, STAT6 VT, furin^fl/fl and CD4 Cre x furin^fl/fl mice were isolated 3 weeks post infection. Cells were suspended at 2×10⁷ cells/ml in PBS with 2% FCS, and Fc receptors were blocked with a 2.4G2 mAb (Clone: 145–2C11, eBiosciences). Antibodies for surface staining were: anti-CD3 PE-Cy7, anti-CD3 FITC (Clone: 145–2C11, eBiosciences), anti-CD4 PE-Cy7 (Clone: GK1.5, eBioscience), anti-LAP PE (Clone: TW7–20B9 BioLegend) vs. isotype IgG1κ PE and anti-GARP PE (Clone: F011–5, BioLegend). For intracellular Foxp3 staining, the Foxp3 staining buffer (Clone: FJK-16S, eBioscience) and anti-Foxp3 PE, Foxp3 PE-Cy7 and Foxp3 APC antibodies were used in accordance with the manufacturer’s instructions.
In experiments involving BMT, spleen and MLN cells from uninfected and Hpb-infected BALB/c or STAT6^–/– mice were isolated on day 6 post-infection and stained as detailed above. These experiments utilized, in addition, anti-H2b PE, anti-H2d PE, and anti-H2b APC antibodies (Clones: SF1–1.1, SF1–1.1.1, AF6.88.5 BD Biosciences).

Li Y., Liu W., Guan X., Truscott J., Creemers J.W., Chen H.L., Pesu M., El Abiad R.G., Karacay B., Urban JF J.r., Elliott D.E., Kaplan M.H., Blazar B.R, & Ince M.N. (2018). STAT6 and Furin Are Successive Triggers for the Production of TGFβ by T Cells. Journal of immunology (Baltimore, Md. : 1950), 201(9), 2612-2623.

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