35 mm confocal dish

Manufactured by SPL Life Sciences

The 35-mm confocal dish is a laboratory equipment designed for cell culture and microscopy applications. It provides a contained and controlled environment for observing cells under a confocal microscope.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Applied precision deltavision elite, by Cytiva (1 mentions) Deltavision algorithms, by Cytiva (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Biotinylated goat anti mouse igg antibody, by Vector Laboratories (1 mentions) Streptavidin alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Hoechst 33342 dye, by Thermo Fisher Scientific (1 mentions) R37601, by Thermo Fisher Scientific (1 mentions)

4 protocols using 35 mm confocal dish

Visualizing siRNA and Peptide Nanocomplex Internalization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cellular internalization of the siRNA/peptide nanocomplex was investigated using confocal imaging. HeLa cells of 2.0 × 10⁴ were incubated in a 35-mm confocal dish (SPL Life Sciences Co., Ltd., Pocheon, South Korea) for 24 h. After a 30-min incubation of the final 50 nM Cy3-labeled siRNA and fluorescein (FITC)-labeled S-R15 (20:1 N/P ratio), the
nanocomplex was applied to the cells for 4 h. The nucleus and actin were stained using 5 µg/mL Hoechst 33342 and 100 nM SiR-actin kit, respectively. The intracellular localization and co-localization of siRNA and S-R15 were confirmed using fluorescence and a confocal microscope (Ti2; Nikon, Tokyo, Japan). Both of them were analyzed at the single-molecule level using super-resolution radial fluctuation (SRRF). Bright-field and fluorescence images were acquired at 900× magnification. ImageJ software was used to merge the fluorescence images of Cy3, FITC, and SiR-actin [37 (link)].

Ryu Y.C., Kim K.A., Kim B.C., Wang H.M, & Hwang B.H. (2021). Novel fusion peptide‐mediated siRNA delivery using self‐assembled nanocomplex. Journal of Nanobiotechnology, 19, 44.

+ Open protocol

+ Expand

Lysosome Calcium Measurement with GCaMP3-ML1

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysosome calcium measurement was performed using methods described previously [10 (link)]. Briefly, 2 × 10⁵ HT1080 cells stably expressing GCaMP3-ML1 were cultured in a 35-mm confocal dish (SPL Life Sciences, 100350). Changes in cytosolic Ca²⁺ levels were monitored by following changes in GCaMP3-ML1 fluorescence for 15 min upon addition of 200 μM GPN in Ca²⁺-free external solution containing 145 mM NaCl, 5 mM KCl, 3 mM MgCl₂, 10 mM glucose, 1 mM EGTA (Sigma-Aldrich, E3889), 20 mM HEPES (Gibco, 15630080), pH 7.4, using the real-time mode of epifluorescence microscopy (Applied Precision DeltaVision Elite, Applied Precision Inc., USA). Data Inspection Program provided by the DeltaVision software was used to measure the intensity of GCaMP3-ML1 fluorescence, and the mean fluorescence intensity was monitored at 488 nm. The acquired epifluorescence images were numerically deconvolved using DeltaVision algorithms (Applied Precision Inc., USA).

Kim H.K., Lee G.H., Bhattarai K.R., Lee M.S., Back S.H., Kim H.R, & Chae H.J. (2020). TMBIM6 (transmembrane BAX inhibitor motif containing 6) enhances autophagy through regulation of lysosomal calcium. Autophagy, 17(3), 761-778.

+ Open protocol

+ Expand

Immunofluorescence Assay for PEDV Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Vero cells were maintained in α-MEM media supplemented with 5% heat-inactivated FBS and 1% antibiotic-antimycotic agent, and cultivated on coverslips placed in 35 mm confocal dish (SPL, Korea) for the immunofluorescence assay. After cells reached confluence, PEDV SM98 strain was inoculated onto the cell monolayer at multiplicity of infection (MOI) of 1 and incubated for 1 h at 37 °C. Subsequently, serum-free α-MEM media containing trypsin (2.5 μg/ml) was added and the culture was incubated for 3 days at 37 °C. Then, cells were fixed with 4% paraformaldehyde for 10 min at room temperature. Cells were subsequently blocked with 1% bovine serum albumin in PBS for 1 h at room temperature and then incubated with mouse anti-S25-229 immune sera for 1 h at room temperature. Then, cells were incubated with biotinylated goat anti-mouse IgG antibody at a dilution of 1: 200 (Vector, USA) followed by streptavidin-Alexa Fluor 488 (Invitrogen, USA) at a dilution of 1: 500. Finally, cells were counterstained with Hoechst 33342 dye (Invitrogen, USA) for 1 min, and cell staining was visualized using a confocal laser scanning microscopy LSM710 (Carl Zeiss, USA).

Piao D.C., Lee Y.S., Bok J.D., Cho C.S., Hong Z.S., Kang S.K, & Choi Y.J. (2016). Production of soluble truncated spike protein of porcine epidemic diarrhea virus from inclusion bodies of Escherichia coli through refolding. Protein Expression and Purification, 126, 77-83.

+ Open protocol

+ Expand

Photodynamic Therapy of U87MG Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To visualize the frequency of live cells after treatment with KUP-1 (0.4 mg mL⁻¹) and O₂@ KUP-1 (0.4 mg mL⁻¹) under irradiation at 530 nm (60 mW cm^-2) for 3 min, U87MG cells (2 × 10⁴) were plated on a 35-mm confocal dish (SPL, Korea). When the cells were plated on the dish, they were treated with KUP-1 and O₂@ KUP-1 in SF media. After incubation for 12 h, the cells were rinsed twice with 1 × PBS and irradiated with SF media at 530 nm. The cells on SF media were irradiated at 530 nm (60 mW cm⁻²) for 3 min, and the cells were incubated for 30 h at 37 °C with 5% CO₂. The frequency of live cells was determined according to the manufacturer’s protocol (Cat #R37601, Thermo Fisher, USA).

Shin J., Kang D.W., Lim J.H., An J.M., Kim Y., Kim J.H., Ji M.S., Park S., Kim D., Lee J.Y., Kim J.S, & Hong C.S. (2023). Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation. Nature Communications, 14, 1498.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!