The o-cresolphthalein complex one (OCPC) method was used to quantify calcium deposition by the MSC in microgels as previously described14 (link). Briefly, the samples were homogenized in 1X Tris–EDTA buffer and centrifuged at 10,000g for 10 min. The supernatant was collected and mixed with a working solution containing 5% ethanolamine/boric acid buffer (Sigma), 5% o-cresolphthalein (Sigma), and 2% hydroxyquinoline (Sigma) in distilled water. The mixture was incubated at room temperature in the dark for 10 min, and the absorbance was read at 575 nm. CaCl2 (Sigma) dissolved in water at different concentrations served as standards. Microgels with HA in the formulation were excluded from this assay.
O cresolphthalein
Manufactured by Merck Group
Sourced in Canada
O-cresolphthalein is a laboratory reagent used as a colorimetric indicator. It is a chemical compound that changes color in response to changes in pH, making it useful for various analytical and testing applications in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Ethanolamine boric acid buffer, by Merck Group (2 mentions)
Cacl2, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Gluconolactone, by Merck Group (1 mentions)
Amino acid, by Merck Group (1 mentions)
Glycerophosphate, by Merck Group (1 mentions)
Cyquant cell proliferation kit, by Thermo Fisher Scientific (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Alizarin red stain, by Merck Group (1 mentions)
Potassium phosphate dibasic, by Merck Group (1 mentions)
Sodium iodide, by Merck Group (1 mentions)
Imidazole, by Merck Group (1 mentions)
Cadmium chloride, by Merck Group (1 mentions)
Sodium potassium tartrate, by Merck Group (1 mentions)
Copper sulphate, by Merck Group (1 mentions)
Magnesium chloride, by Merck Group (1 mentions)
L alanine, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Potassium iodide, by Merck Group (1 mentions)
7 protocols using o cresolphthalein
1
Quantifying Calcium Deposition in MSC Microgels
2
Porcine Skin Barrier Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Amino acids, o-cresolphthalein, calcium chloride, and gluconolactone were purchased from Sigma-Aldrich. The porcine skin (Micropig, back skin, 2.5 × 2.5 cm2, 1 mm thickness) was obtained from MediKinetics (Pyeongtaek, South Korea). Phosphate buffered saline (PBS) was purchased from GIBCO (New York, USA). The human skin equivalent (Neoderm-E) was constructed by TEGO Science (Seoul, South Korea). The antibodies against Dsg1, Dsc1, and α-Tubulin were purchased from Thermo Fisher (MA, USA), Cloud-Clone (TX, USA), and Santa Cruz Biotechnology (MN, USA). The tape strip (D-Squame D100) for sampling stratum corneum was obtained from Cuderm (TX, USA). All other molecular biology reagents were purchased from commercial sources and were of analytical grade. The serine for human in vivo test was purchased from Evonik (Essen, Germany) and was of cosmetic grade. Human testing was in accordance with the Declaration of Helsinki.
3
Branched PEI-Mediated BMP-2 Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Branched PEIs of 2 kDa and 25 kDa were acquired from Sigma. mTAT, the HIV-1 TAT (RKKRRQRRRR) covalently fused with ten histidine and two cysteine residues (C-5H-Tat-5H-C), was synthesized and obtained from Changxi Biology Ltd. (Shanghai, China). The alkaline phosphatase (ALP) substrate p-nitrophenol phosphate (p-NPP), 8-hydroxyquinoline, o-cresolphthalein, 2-amino-2-methyl-propan-1-ol (AMP), dexamethasone, glycerophosphate, and ascorbic acid (AA) were also obtained from Sigma. The commercially available BMP-2 expression vector pCMV6-XL4-BMP2 was obtained from Origene (Rockville, MD). We obtained the blank (control) gWiz plasmid and its GFP expressing derivative gWiz-GFP from Aldevron Gene Therapy Systems, Inc. (San Diego, US). A CyQUANT cell proliferation kit was from Molecular Probes (Portland, OR). A BMP-2 ELISA kit was obtained from Wuhan Boster (Wuhan Boster, PR China). Phospho-Smad1/5/8 primary antibodies were purchased from Abcam (Cambridge, US).
4
Vascular Smooth Muscle Cell Calcification
5
Quantifying Calcium Deposition in AD-MSC Microgels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The o-cresolphthalein complex one (OCPC) method was used to quantify calcium deposition by the AD-MSC on microgels as previously described (26 (link)). Briefly, the samples were homogenized through sonication in 1X Tris-HCl buffer and centrifuged at 10,000 g for 10 min. The supernatant was then collected and mixed with a working solution containing 5% ethanolamine/boric acid buffer (Sigma), 5% o-cresolphthalein (Sigma), and 2% hydroxyquinoline (Sigma) in DI water. The mixture was incubated at room temperature in the dark for 10 min, and the absorbance was read at 575 nm. CaCl2 (Sigma) dissolved in DI water at different concentrations served as standards.
6
Biochemical Assays Utilizing Common Laboratory Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cadmium chloride (CdCl 2, 99%), silver nitrate (AgNO 3, 99.9%), potassium dihydrogen phosphate (KH 2 PO 4 , 99.5%), dibasic potassium phosphate (K 2 HPO 4, 99.95%), ascorbic acid (vitamin C, 99.9%), ethylenediaminetetraacetic acid (EDTA, 99.0%), 2thiobarbituric acid (TBA, 97.0%), salicylic acid (C₇H₆O₃, 95.5%), 5,5′-dithiobis-2-nitrobenzoic acid (DTNB, 98.28%), 4-nitro blue tetrazolium chloride (NBT, 99.9%), hydrogen peroxide (H 2 O 2 , 99.9%), ethanolamine (C 2 H 7 NO, 99%), o-cresolphthalein (C 22 H 18 O 4 , 95%), 8-hydroxyquinoline (C 9 H 7 NO, 99.99%), sodium potassium tartrate (KNaC 4 H 4 O 6 .4H 2 O, 99%), sodium iodide (NaI, 99%) potassium iodide (KI, 99.99%), copper sulphate (CuSO₄,98%), sodium hydroxide (NaOH 99.99%), imidazole (C 3 H 4 N 2 , 99%), pyruvic acid (C 3 H 4 O 3 , 99%), Tris pH 7.8 (C 4 H 11 NO 3 , 99.9%), L-alanine (C 3 H 7 NO 2 , 99%), diethanolamine pH 10.4 (C 4 H 11 NO 2 , 90%), chloroform (CHCl 3 , 94%) and magnesium chloride (MgCl 2 , 98%) were from Sigma-Aldrich.
7
Microcystin-LR Degradation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microcystin-LR (MC-LR) was purchased from Cayman Chemicals (Ann Arbor, Michigan, MI, USA) and a stock solution of 50 mg/mL was made by diluting 100 μg lyophilized film of MC-LR (as supplied) using 2 mL of methanol, stored at -20 °C. Crystal violet and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Aldrich, (Ontario, Canada). Various reagents for preparing the stock solution of iron, magnesium and copper (divalent form) including: ferrous ammonium sulphate hexahydrate (iron source), o-phenanthroline, magnesium sulphate (magnesium source), o-cresolphthalein, barium chloride, ethylenediamine tetraacetate, potassium cyanide, and copper sulphate pentahydrate (copper source), were purchased from Sigma Aldrich, (Ontario, Canada). Quartz sand used as the filter media was obtained from Chemin Ste-Foy DWTP, Quebec City, Canada. MC-degraders: Arthrobacter ramosus (NRRL B-3159), Bacillus sp. (NRRL B-14393) and Sphingomonas sp.
(NRRL B-59555) were purchased from NRRL Agricultural Research Service (ARS) culture collection. All the analytical reagents used in preparing nutrient and culture media, LC-MS grade solvents and reagents used to prepare analytical mobile phases, were purchased from Fisher Scientific, (Ontario, Canada).
(NRRL B-59555) were purchased from NRRL Agricultural Research Service (ARS) culture collection. All the analytical reagents used in preparing nutrient and culture media, LC-MS grade solvents and reagents used to prepare analytical mobile phases, were purchased from Fisher Scientific, (Ontario, Canada).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!