Anti ifn γ

Manufactured by ABclonal

Sourced in China

Anti-IFN-γ is a laboratory reagent used for the detection and quantification of the interferon-gamma (IFN-γ) protein. It is a specific antibody that binds to IFN-γ and can be used in various immunoassay techniques, such as ELISA, to measure the levels of this cytokine in biological samples.

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Lab products found in correlation

Granzyme b, by Bioss Antibodies (1 mentions) Anti sting, by Cell Signaling Technology (1 mentions) Anti p tbk1, by Cell Signaling Technology (1 mentions) Anti il 17a, by Bioworld Technology (1 mentions) Anti p sting, by Cell Signaling Technology (1 mentions) Anti γδ tcr, by Thermo Fisher Scientific (1 mentions) Anti nak tbk1, by Abcam (1 mentions) β actin, by Proteintech (1 mentions) Enhanced chemiluminescence detection kit, by Applygen (1 mentions) Ecl plus western blotting detection reagents, by Merck Group (1 mentions) Anti tnf α, by Bioss Antibodies (1 mentions) Anti il 4, by Bioss Antibodies (1 mentions) Anti β actin, by Bioss Antibodies (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Bicinchoninic acid assay kit, by Beyotime (1 mentions)

3 protocols using anti ifn γ

Immunohistochemical Analysis of Immune Markers

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Immunohistochemistry
(IHC) staining was carried out based on the previously described studies.^{31 (link),32 (link)} The primary antibody included anti IFN-γ (catalog number A12450,
1:100 dilution, ABclonal), Granzyme B (catalog number bs-1351R, 1:500
dilution, Bioss), and Ki-67 (catalog number GB13030-2, 1:1000 dilution,
Servicebio). After diaminobenzidine (#ZLI9107, ZSGB-BIO, Beijing,
China) staining and hematoxylin counterstaining, the tissue photos
were captured with an Olympus microscope (Olympus, Tokyo, Japan).

Guo W., Gao H., Li H., Ge S., Zhang F., Wang L., Shi H, & Han A. (2022). Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer. ACS Applied Materials & Interfaces, 14(28), 31634-31644.

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Western Blot Analysis of Oral Lichen Planus

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Fresh tissue samples from patients with OLP and healthy controls were carefully dissected. Protein concentrations were measured according to a BCA assay (Thermo Scientifc, Waltham, MA, USA) generated standard curve. A total of 40 µg of protein was denatured and then subjected to 12% SDS-polyacrylamide gel electrophoresis, followed by transfer onto polyvinylidene fuoride membranes (Millipore Corporation, Billerica, MA, USA). The membranes were incubated with primary antibodies (anti-γδ TCR (Thermo Fisher, Catalog #5A6.E9), anti-STING (Cell Signaling Technology, Catalog #D2P2F), anti-NAK/TBK1 (Abcam, Catalog #EP611Y), anti-p-STING (Cell Signaling Technology, Catalog #E9A9K), anti-p-TBK1 (Cell Signaling Technology, Catalog #D52C2), anti-IFN-γ (Abclonal, Wuhan, China, Catalog #A12450), anti-IL-17A (Bioworld, Dublin, OH, USA, Catalog #BS6041), anti-HLA-DR (Nonus, CO, USA, Catalog #NBP2-67610) and anti-CCR6 (Bioss, Beijing, China, Catalog #BS-1542R)). After being washed, the membranes were then probed with secondary antibodies. Next, the blots were stained using an enhanced chemiluminescence detection kit (Applygen, Beijing, China). The relative protein levels were calculated based on β-actin (Proteintech, Chicago, IL, USA, Catalog #66009-1-Ig) as the loading control and were densitometrically analyzed by Image J software (NIH, Bethesda, MD, USA).

Huang S., Tan Y.Q, & Zhou G. (2023). Aberrant Activation of the STING-TBK1 Pathway in γδ T Cells Regulates Immune Responses in Oral Lichen Planus. Biomedicines, 11(3), 955.

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Gestational Immune Profile Analysis

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Total protein was extracted from spleen tissues isolated from five pregnant CBA/J mice from each group on day 14 of gestation using RIPA lysis buffer (Beyotime Institute of Biotechnology). Protein (30 µg/lane) was quantified using a bicinchoninic acid assay kit (Beyotime Institute of Biotechnology) and separated by 10% SDS-PAGE and transferred to PVDF membranes. The membranes were blocked with 10% skim milk at room temperature for 4 h. Subsequently, the membranes were incubated at 4°C overnight with the following primary antibodies: Anti-FoxP3 (cat. no. bs-0269R; 1:1,000; BIOSS), anti-TNF-α (cat. no. A0277; 1:1,000; ABclonal Biotech Co., Ltd.), anti-IFN-γ (cat. no. bs-0480R; 1:1,000; BIOSS), anti-IL-4 (bs-0581R; 1:1,000; BIOSS) and anti-β-actin (cat. no. AC026; 1:2,000; ABclonal Biotech Co., Ltd.). Subsequently, the membranes were incubated with a goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (1:2,000; cat. no. bs-0295G-HRP; BIOSS). Protein bands were visualized using ECL Plus Western Blotting Detection reagents (EMD Millipore). Blots were performed in triplicate and protein expression was quantified using ImageJ 2× software (National Institutes of Health) with β-actin as the loading control.

Li G., Yang L., Li D., Zhang J., Du L., Xia L., Liu Y, & Hu W. (2020). Effects of combined treatment with PD-L1 Ig and CD40L mAb on immune tolerance in the CBA/J × DBA/2 mouse model. Molecular Medicine Reports, 21(4), 1789-1798.

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