C43 ab128193

Manufactured by Abcam

C43] ab128193 is a rabbit monoclonal antibody that recognizes the C43 protein. The antibody is suitable for use in various immunochemical techniques, such as Western blotting and immunocytochemistry, to detect the expression of the C43 protein in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Ab129002, by Abcam (2 mentions) Gtx125926, by GeneTex (2 mentions) Odyssey imaging system, by LI COR (2 mentions) Irdye 800cw, by Abcam (2 mentions) F1804, by Merck Group (2 mentions) Ab216772, by Abcam (1 mentions) Free protease inhibitor cocktail tablet, by Roche (1 mentions) Ab216777, by Abcam (1 mentions) Irdye 680rd, by Abcam (1 mentions) Irdye 680rd goat, by Abcam (1 mentions) Protease inhibitor cocktail tablet, by Roche (1 mentions)

2 protocols using c43 ab128193

Visualization of Protein Expression in Mini-Genome Assays

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To visualise protein expression during mini-genome assays, around 500,000 transfected cells were lysed in RIPA buffer (150mM NaCl, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SDS, 50mM TRIS, pH 7.4) supplemented with an EDTA-free protease inhibitor cocktail tablet (Roche).
Proteins were detected with mouse α-FLAG (F1804, Sigma), rabbit α-Vinculin (AB129002, Abcam), rabbit α-PB2 (GTX125926, GeneTex) and mouse α-NP ([C43] ab128193, Abcam). The following near infrared (NIR) fluorescently tagged secondary antibodies were used: IRDye® 680RD Goat Anti-Rabbit (IgG) secondary antibody (Ab216777, Abcam) and IRDye® 800CW Goat Anti-Mouse (IgG) secondary antibody (Ab216772, Abcam). Western Blots were visualised using an Odyssey Imaging System (LI-COR Biosciences).

Golshani P., Liu X.B, & Jones E.G. (2001). Differences in quantal amplitude reflect GluR4- subunit number at corticothalamic synapses on two populations of thalamic neurons. Proceedings of the National Academy of Sciences of the United States of America, 98(7), 4172-4177.

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Visualizing Protein Expression in Mini-Genome Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

To visualize protein expression during mini-genome assays, around 500,000 transfected cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris [pH 7.4]) supplemented with an EDTA-free protease inhibitor cocktail tablet (Roche).
Proteins were detected with mouse α-FLAG (F1804, Sigma), rabbit α-Vinculin (AB129002">AB129002, Abcam), rabbit α-PB2 (GTX125926, GeneTex), and mouse α-NP ([C43] AB128193, Abcam). The following near-infrared (NIR) fluorescently tagged secondary antibodies were used: IRDye 680RD goat anti-rabbit (IgG) secondary antibody (AB216777, Abcam) and IRDye 800CW goat anti-mouse (IgG) secondary antibody (AB216772, Abcam). Western blots were visualized using an Odyssey Imaging System (LI-COR Biosciences).

Peacock T.P., Sheppard C.M., Lister M.G., Staller E., Frise R., Swann O.C., Goldhill D.H., Long J.S, & Barclay W.S. (2023). Mammalian ANP32A and ANP32B Proteins Drive Differential Polymerase Adaptations in Avian Influenza Virus. Journal of Virology, 97(5), e00213-23.

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