Ccnb1

Manufactured by Merck Group

Sourced in United States

CCNB1 is a protein-coding gene that plays a crucial role in cell cycle regulation. It encodes a regulatory subunit of the cyclin-dependent kinase complex, which is involved in the transition from G2 phase to mitosis. The CCNB1 product is essential for the initiation and progression of the cell division process.

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Lab products found in correlation

2 protocols using ccnb1

Western Blot Antibody Panel

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The following antibodies should be utilized for Western blot analysis. ANP32B (cat. No. ab200836; Abcam, USA), ANP32A (cat. No. 15810-1-AP; Proteintech, USA) and C-MYC (cat. No. 9402S; Cell Signal Technology, USA), PLK1 (cat. No. ab17056; Abcam, USA), Anti-acetyl-Histone H3 antibody (cat. No. 06-599; Millipore, USA), Histone H3 (D1H2) XP^® Rabbit mAb (cat. No. 4499S; Cell Signal Technology, USA), H3K27ac (cat. No. ab4729; Abcam, USA), Monoclonal ANTI-FLAG M2 antibody (Lot#SLBS3530V; Sigma, GER), CCNB1(cat. No. ab32053; Abcam, USA), CDK2 (cat. No. ab32147; Abcam, USA), BAX (cat. No. ab32503; Abcam, USA). The reference protein used was GAPDH (cat. No. MA3374; Millipore).

Wan X., Wang J., Fang F., Hu Y., Zhang Z., Tao Y., Zhang Y., Yu J., Wu Y., Zhou B., Yin H., Ma L., Li X., Zhuo R., Cheng W., Zhang S., Pan J., Lu J, & Hu S. (2024). Super enhancer related gene ANP32B promotes the proliferation of acute myeloid leukemia by enhancing MYC through histone acetylation. Cancer Cell International, 24, 81.

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High-Throughput Screening of Mitotic Regulators

Cited 1 time

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The drugs used for screening in this study are listed in Supplementary Table 1.
Reversine, SP600125, ZM 447439, BI 2536, STLC, Dimethylenastron, SB203508, RO 3306, Cdk1 Inhibitor III, Roscovitine, NSC 95397, IPA3, Y-27632, ITX-3, Nocodazole, Paclitaxel, Blebbistatin, Cytochalasin B, MG 132 and Velcade were purchased from Sigma–Aldrich (St. Louis, MO, USA). AZ 3146 and Mps-BAY2a were obtained from Tocris (Bristol, United Kingdom). MLN8237 (Alisertib), AZD1152-HQPA (Baraserib) and SB743921 were purchased from Selleckchem. GSK923295 is from MedChem express.
Monoclonal antibodies against α-tubulin, CDK1, AURKA, PLK1, KIF11 (Sigma-Aldrich); CCNB1, CCNE1, TP53, CDKN1A, JNK1, JNK2 (Cell signaling); EB1, COFILIN (Santa Cruz), TTK, BUBR1 (Abcam). Polyclonal antibodies against PRC1 (Santa Cruz), Tpx2 [69 (link)]; Kif23 [70 (link)] were used.

Jemaà M., Abdallah S., Lledo G., Perrot G., Lesluyes T., Teyssier C., Roux P., van Dijk J., Chibon F., Abrieu A, & Morin N. (2016). Heterogeneity in sarcoma cell lines reveals enhanced motility of tetraploid versus diploid cells. Oncotarget, 8(10), 16669-16689.

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