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3 protocols using leukotriene c4

1

Lipid Synthesis and Signaling Assay

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Indomethacin, ibuprofen, mevalonic acid, mevastatin, S1P, and glutathione were purchased from Sigma. HPLC-grade solvents were purchased from Fisher. Leukotriene C4, D4, and geranylgeranyl-pyrophosphate was purchased from Cayman Chemical. LPI was purchased from Avanti Polar Lipids. ETYA and AACOCF3 were purchased from Biomol. Ggg was chemically synthesized using the protocol specified in the section below.
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2

Lipid Synthesis and Signaling Assay

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Indomethacin, ibuprofen, mevalonic acid, mevastatin, S1P, and glutathione were purchased from Sigma. HPLC-grade solvents were purchased from Fisher. Leukotriene C4, D4, and geranylgeranyl-pyrophosphate was purchased from Cayman Chemical. LPI was purchased from Avanti Polar Lipids. ETYA and AACOCF3 were purchased from Biomol. Ggg was chemically synthesized using the protocol specified in the section below.
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3

Signaling Pathways in Immune Cells

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Reagents. CysLTR1 antagonist (Montelukast) and leukotriene C 4 were purchased from Cayman Chemical Company, (Ann Arbor, MI, USA). P2Y12 antagonist (MRS2395) and 2-(Methylthio)-ADP were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Antibodies against phospho-PLC γ 2, phospho-Akt, phospho-ERK, ERK, and c-Fos were purchased fromCell Signaling Technology, Inc., (Danvers, MA, USA). Antibody against NFATc1 was purchased from Santa Cruz Biotechnology, Inc. Antibody against phospho-CREB was purchased from EMD Millipore (Billerica, MA, USA). Antibody against β-actin was purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). All other reagents were from Sigma-Aldrich; Merck KGaA.
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