Smoc2 sc 376104

Transforming growth factor-β (TGF-β, 763104) was purchased from Biolegend (San Diego, CA, USA). Isoprenaline (ISO, I5627) was obtained from Sigma-Aldrich and prepared in DD water. The SMOC2 (SC-376104, 1:500 for western blot and 1:100 for satining) and ILK (SC-20019, 1:500) antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Antibodies against collagen type I (Col I, 1:1000), collagen type III (Col III, 1:1000), phospho-JNK (Tyr185,1:1000), JNK (1:2000), phospho-p38 MAPK (Thr180/Tyr182,1:1000), t-p38 (1:1000), phospho-AKT (1:5000), t-AKT (1:5000) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH,1:4000) were obtained from Proteintech (Wuhan, China). Anti-α smooth muscle actin (a-SMA, 1:100 for staining) antibody was purchased from Abcam (Cambridge, UK).

HEK293 cells were purchased from ATCC (VA, USA). Cells were maintained in complete Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 ug/ml streptomycin at 37°C in 5% CO₂. Primary antibodies against β-actin (sc-47778), OPN (sc-21742), and SMOC2 (sc-376104) were purchased from Santa Cruz Biotechnology (Shanghai, China). Primary antibodies against RUNX2 (12556S), p-AKT1/2/3 (4060S), and AKT1/2/3 (9272) were purchased from Cell Signaling Technology (Shanghai, China). A primary antibody against FAK (66258-1-Ig) was purchased from Proteintech. A primary antibody against p-FAK (ab81298) was ordered from Abcam, and the PI3K inhibitor LY294002 was purchased from MCE (USA).