DOX was purchased from Sigma (Santa Clara, CA, USA). Tissue Protein Extraction Kit was obtained from KEYGEN Biotech. Co., Ltd. (Nanjing, China). The bicinchoninic acid (BCA) protein assay kit, ROS assay kit, cell lysis buffer and phenylmethanesulfonyl fluoride (PMSF) were obtained from Beyotime Institute of Biotechnology (Jiangsu, China). Creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) detection kits were purchased from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). Sodium dodecyl sulfate (SDS), hydroxymethyl aminomethane (Tris) and 4′,6′-Diamidino-2-phenylindole (DAPI) were purchased from Sigma (St. Louis, MO, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was provided by Roche Diagnostics (Basel, Switzerland). Lipofectamin2000, TransZolTM, TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal), TransStart® Top Green qPCR SuperMix were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). SanPrep Column MicroRNA Mini-Preps Kit, MicroRNA First Strand cDNA Synthesis Kit and MicroRNAs Quantitation PCR Kit were purchased from Sangon Biological Engineering Technology & Services Co., Ltd. (Shanghai, China).
Glutathione peroxidase gsh px detection kits
Manufactured by Nanjing Jiancheng
Sourced in China
Glutathione peroxidase (GSH-Px) detection kits are lab equipment used to measure the activity of the enzyme glutathione peroxidase in biological samples. Glutathione peroxidase is an important antioxidant enzyme that helps protect cells from oxidative stress.
Automatically generated - may contain errors
Lab products found in correlation
Phenylmethanesulfonyl fluoride pmsf, by Beyotime (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt, by Roche (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Ros assay kit, by Beyotime (1 mentions)
Cell lysis buffer, by Beyotime (1 mentions)
Bicinchoninic acid bca protein assay kit, by Beyotime (1 mentions)
Tissue protein extraction kit, by Keygen Biotech (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Isoproterenol hydrochloride, by Merck Group (1 mentions)
Anti jnk antibody, by Cell Signaling Technology (1 mentions)
Anti il 1 antibody, by Abcam (1 mentions)
Anti il 18 antibody, by Abcam (1 mentions)
Anti wnt5a antibody, by Abcam (1 mentions)
Anti nlrp3 antibody, by Cell Signaling Technology (1 mentions)
Anti il 6 antibody, by Abcam (1 mentions)
Superoxide dismutase sod, by Nanjing Jiancheng (1 mentions)
Sirt1 sirna, by RiboBio (1 mentions)
Cell lysis buffer for western and ip, by Beyotime (1 mentions)
4 protocols using glutathione peroxidase gsh px detection kits
1
Cellular Stress and Antioxidant Assays
2
Antioxidant Mechanisms in Cellular Systems
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Acrylamide (ACR), melatonin (MT), and other chemicals (all 99.9% purity) were obtained from Sigma Chemical Co. All solvents were of analytical grade and Milli-Q water was used throughout the analyses. Malonaldehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) detection kits were provided by the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Fluorescent probe 2,7-dichlorofluorescein diacetate (DCFH-DA), Hoechst 33258 dye, normal-melting-point agarose (NMPA) gel, low-melting-point agarose (LMPA) gel, and ethidium bromide (EB) were from Sigma Co. RPMI-1640 culture medium and fetal bovine serum were purchased from GIBCO. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) cell viability assay kit was from Promoter Biotechnology Inc. (Nanjing, China).
3
Molecular Mechanisms of SFRP5 in Inflammation
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Recombinant human SFRP-5 was obtained from R&D Systems (MN, USA). Isoproterenol hydrochloride was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Wnt5A antibody, anti-SFRP5 antibody, anti-IL-1 antibody, anti-IL-6 antibody, and anti-IL-18 antibody were purchased from Abcam (Cambridge, UK). An anti-glyceraldehyde-3 phosphate dehydrogenase (GAPDH) antibody was purchased from Bioworld (MN, USA). Anti-NLRP3 antibody and anti-JNK antibody were purchased from Cell Signaling Technology (Danfoss, MA, USA). Superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) detection kits were purchased from Nanjing Jiancheng Institute of Biological Engineering (Nanjing, China).
4
Biochemical Markers of Oxidative Stress
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Creatinine (Cr), blood urea nitrogen (BUN), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH-Px) detection kits were purchased from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). Enhanced Bicinchoninic Acid (BCA) Protein Assay Kit, Reactive Oxygen Species Assay Kit, Cell lysis buffer for Western and IP and phenylmethanesulfonyl fluoride (PMSF) were obtained from Beyotime Institute of Biotechnology (Jiangsu, China). Sodium dodecyl sulfate (SDS), hydroxymethyl aminomethane (Tris) and 4′,6′-Diamidino-2-phenylindole (DAPI) were purchased from Sigma (St. Louis, MO, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was provided by Roche Diagnostics (Basel, Switzerland). Hematoxylin, 2-step plus®Poly-HRP Anti-Mouse/Rabbit IgG Detection System and 3,3′-Diaminobenzidine tetrahydrochloride (DAB) Substrate Kit were provided by Zhongshan Golden Bridge Biotechnology (Beijing, China). TransZol™, TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR(One-Step gDNA Removal) and TransStart® Top Green qPCR SuperMix were supplied by Beijing TransGen Biotech Co., Ltd. (Beijing, China). Sirt1 siRNA (Sequence: CCACCTGAGTTGGATGATA) was purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China), and Lipofectamine 2000 was obtained from GenePharma (Shanghai, China).
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