Anti cd36

Flow cytometry was performed on mouse blood as previously described (63 (link)). Briefly, to obtain single-cell suspensions, spleen samples were ground between sterile frosted glass slides in 7 ml of red blood cell lysis buffer (0.15 mM NH₄Cl, 10 mM KHCO3, 0.1 mM disodium ethylenediaminetetraacetic acid, pH 7.2) and then filtered through sterile nylon mesh. Whole blood, obtained by cardiac puncture, was treated with red blood cell lysis buffer twice. Cell pellets were washed and resuspended in phosphate-buffered saline containing 2 mM ethylenediaminetetraacetic acid and 0.5% bovine serum albumin. Fluorophore-conjugated antibodies with specificity to mouse cell antigens were as follows: anti-CD11b (M1/70) (BD Biosciences Cat# 550993, RRID:AB_394002), anti-CD11c (HL3) (BD Biosciences Cat# 561022, RRID:AB_2033997), anti-SiglecF (E50-2440) (BD Biosciences Cat# 552125, RRID:AB_394340), anti-Ly6G (1A8) (BD Biosciences Cat# 561236, RRID:AB_10611860), and anti-CD36 (JC63.1) (Cayman Chemical Cat# 10009870, RRID:AB_10342682). Live/dead fixable dead cell stains (ThermoFisher) were used to exclude dead cells. CD11b+/c+ cells were analyzed for CD36 expression. Paraformaldehyde-fixed cells were acquired using a Becton Dickinson (BD) LSR Fortessa flow cytometer (BD Biosciences, University of Alberta Flow Cytometry core) and analyzed with FlowJo (version 10) software (RRID:SCR_008520).

Bleomycin, DNase I, paraformaldehyde, mouse IgG, and mouse serum were purchased from Sigma-Aldrich (St. Louis, MO, USA), and simvastatin, obtained from the Cayman Chemical Co. (Ann Arbor, MI, USA), was used as indicated.). Dispase was obtained from Corning Incorporated (Corning, NY, USA). Antibodies for western blotting were as follows: anti-CD36, anti-MMR (Cayman Chemical Co), anti-α- SMA, anti-fibronectin (Abcam, Cambrige, MA, USA), anti-type 1 collagen α2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-α-SMA (Abcam), and anti-β-actin (Sigma-Aldrich).