Rpmi 1640 cell culture media

Manufactured by Merck Group

Sourced in United States

RPMI 1640 is a widely used cell culture medium developed by Moore et al. at Roswell Park Memorial Institute. It is a balanced salt solution designed to support the growth and maintenance of a variety of mammalian cell types in vitro.

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RPMI 1640 (3389 citations) RPMI-1640 media (314 citations) RPMI media (88 citations) Cell culture media (83 citations) Culture media (16 citations) RPMI 1640 culture media (15 citations) 1640 media (5 citations) RPMI culture media (3 citations) RPMI cell culture media (2 citations) RPMI 1640 culture (2 citations)

8 protocols using rpmi 1640 cell culture media

Isolation and Labeling of Circulating Tumor Cells

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Albumin from human serum (HSA), ethanolamine, picoline borane, Hoechts 33342 dye, RPMI 1640 cell culture media, and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St Louis, MO, USA). Fetal bovine serum, fluorescein isothiocyanate (FITC) labeled anti-EpCAM and 1% Penicillin-Streptomycin were from Thermo Fisher Scientific (Waltham, MA, USA). Lysis buffer was purchased from Becton Dickinson (Franklin Lakes, NJ, USA). The glutaraldehyde was from Carl Roth Chemicals (Karlsruhe, Germany) and the absolute ethanol from VWR (Radnor, PA, USA). Human immunoglobulin G1 was obtained from Molecular Innovations (Novi, MI, USA).

Jarvas G., Szerenyi D., Tovari J., Takacs L, & Guttman A. (2021). Modification of Hemodialysis Membranes for Efficient Circulating Tumor Cell Capture for Cancer Therapy. Molecules, 26(16), 4845.

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Comprehensive Biochemical Assays and Cell Culture Protocols

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Cell culture grade DMSO (Cat No D2650), Congo Red (Cat No C6277), Dulbecco’s phosphate buffered saline (DPBS, D8537), Evans Blue (Cat No E2129), β-nicotinamide adenine dinucleotide (NADH, Cat No N4505), oxaloacetate (Cat No O4126) and RPMI 1640 cell culture media (Cat No R8758) were purchased from Sigma-Aldrich. Fulvestrant (Cat No S1191) was purchased from Selleckchem. Lapatinib (HY-50898), nilotinib (Cat No HY-10159), sorafenib (Cat No HY-10201) and vemurafenib (Cat No HY-12057) were purchased from MedChem Express. 3′,3′′,5′,5′′-Tetraiodophenolphthalein (TIPT, Cat No sc-216639) was purchased from Santa Cruz Biotechnology. AmpC β-lactamase was purified from Escherichia coli as previously described.^{30 (link)} Malate dehydrogenase (MDH, Cat No 442610) and CENTA (Cat No 219475) were purchased from EMD Millipore. Trypsin (Cat No T0303) and Suc-Ala-Ala-Pro-Arg-pNA (Cat No L1720) were purchased from Sigma-Aldrich and BACHEM respectively. Cell line MDA-MB-231 (Cat No HTB-26) was purchased from ATCC. Triton X-100 (Cat No 21568-2500) was purchased from Acros. Presto Blue cell viability reagent (Cat No A13262) was purchased from Invitrogen. 96-well plates (Cat No 655096) for DLS were purchased from Greiner Bio-One.

McLaughlin C.K., Duan D., Ganesh A.N., Torosyan H., Shoichet B.K, & Shoichet M.S. (2016). Stable Colloidal Drug Aggregates Catch and Release Active Enzymes. ACS chemical biology, 11(4), 992-1000.

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Macrophage Activation Protocol

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DMF, adenosine 5’-triphosphate disodium hydrate, LPS (LPS 055: B5), ML385, Fetal bovine serum (FBS), RPMI 1640 cell culture media, L-Glutamine, penicillin/streptomycin, phosphate-buffered saline (PBS), and trypsin/EDTA were purchased from Sigma-Aldrich (St. Louis, USA). Ultra-Pure LPS (LPS 0111: B4) were purchased from In vivoGen (San Diego, USA). Supplementary Table 1 contains all antibodies used in this research.

Tastan B., Arioz B.I., Tufekci K.U., Tarakcioglu E., Gonul C.P., Genc K, & Genc S. (2021). Dimethyl Fumarate Alleviates NLRP3 Inflammasome Activation in Microglia and Sickness Behavior in LPS-Challenged Mice. Frontiers in Immunology, 12, 737065.

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Cell Culture Conditions for Breast Cancer Cell Lines

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The human breast cancer cells MDA-MB 468, BT20, MDA-MB 231, MCF7, T47D, SK-BR-3, HEK-293 and mouse 4T1 breast cancer cells were obtained from the American Type Culture Collection. MDA-MB 468 and HEK-293 cells were maintained in DMEM (Sigma) supplemented with 2 μM L-glutamine (Sigma); BT20 cells were maintained in EMEM (Sigma) supplemented with 1 mM Sodium Pyurvate (Sigma); MDA-MB 231, MCF7, T47D, 4T1 and PBMCs were maintained in RPMI 1640 cell culture media (Sigma); SK-BR-3 cells were maintained in DMEM/F12 cell culture media (Sigma). All culture media were supplemented with 10% FBS and 1% Penicillin-Streptomycin (P+S) and cultures were grown at 37 °C with 5% CO_2.

Thakur R., Trivedi R., Rastogi N., Singh M, & Mishra D.P. (2015). Inhibition of STAT3, FAK and Src mediated signaling reduces cancer stem cell load, tumorigenic potential and metastasis in breast cancer. Scientific Reports, 5, 10194.

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Lipid Trafficking Assay in Breast Cancer Cells

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Fulvestrant (Cat. No. S1191) was purchased from Selleck Chemicals. Sorafenib (Cat. No. HY-10201) and vemurafenib (Cat. No. HY-12057) were purchased from MedChem Express. Cell culture grade DMSO (Cat. No. D2650), bovine serum albumin (Cat. No. A7030), IgG from human serum (Cat. No. I4506), insulin from bovine pancreas (Cat. No. I0516), and RPMI 1640 cell culture media (R8758) were purchased from Sigma-Aldrich. Trastuzumab (Herceptin) was obtained from Roche (Mississauga, Ontario, Canada). McCoy’s 5A (Cat. No. 1660082) and DMEM F12 (Cat. No. 11330032) cell culture media, CholEsteryl BODIPY FL C12 (Cat. No. C3927MP), CholEsteryl BODIPY 542/563 C11 (Cat. No. C12680), Hoechst 33342 (Cat. No. H1399), wheat germ agglutinin Alexa Fluor 647 conjugate (Cat. No. W32466), dextran Alexa Fluor 647 conjugates (Cat. No. D22914), and PrestoBlue cell viability reagent (A12361) were purchased from Thermo Fisher Scientific. Cell lines SKOV-3 (Cat. No. HTB-77), MDA-MB-231 (Cat. No. HTB-26), BT-474 (Cat. No. HTB-20), and MCF-7 (Cat. No. HTB-22) were purchased from ATCC. Charcoal-stripped fetal bovine serum (Cat. No. 080750) and Hank’s balanced salt solution (Cat. No. 311515) were purchased from Wisent Bioproducts. Polysorbate 80 (HX2) was purchased from NOF America Corp.

Ganesh A.N., McLaughlin C.K., Duan D., Shoichet B.K, & Shoichet M.S. (2017). A New Spin on Antibody–Drug Conjugates: Trastuzumab-Fulvestrant Colloidal Drug Aggregates Target HER2-Positive Cells. ACS applied materials & interfaces, 9(14), 12195-12202.

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Macrophage Activation and Characterization

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RPMI-1640 cell culture media (#R0833), 200 mM L-glutamine (#G7513), 1% penicillin/streptomycin (#P4333), Accutase (#A6964), mitomycin C (#10107409001), antimycin A from Streptomyces sp. (#A8674), gentamicin (#G1397), dihydroethidium (DHE) (#37291), and gelatin type A (#G1890) were from Sigma-Aldrich. FBS (#P040-37500) and Panexin BMM Serum Substitute (#P04-951SA2) were obtained from PAN Biotech. Ultrapure lipopolysaccharide (LPS) from E. coli K12 (#tlrl-peklps), and ATP (#tlrl-atpl) were purchased from Invivogen. TE buffer (#A0386.1000), and DMSO (#A3672-0250) were from AppliChem, M-CSF (#130-101-705) from Miltenyi Biotech, and mouse recombinant IFN-g (#87389.100) from Biomol.
Primers for qRT-PCR were purchased from Eurofins Genomics. Other chemicals were obtained from either Sigma-Aldrich or Carl Roth unless stated otherwise.

Legroux T.M., Schymik H.S., Gasparoni G., Mohammadi S., Walter J., Libert C., Diesel B., Hoppstädter J, & Kiemer A.K. (2024). Immunomodulation by glucocorticoid-induced leucine zipper in macrophages: enhanced phagocytosis, protection from pyroptosis, and altered mitochondrial function. Frontiers in immunology, 15.

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Synthesis and Characterization of Multifunctional Nanoparticles

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Iron (III) acetylacetonate (Fe(acac)3, 99%) and decanoic acid (DA, 99%) were purchased from Acros.
Dibenzylether (DBE, 98%), acetone (99.5%), chloroform (99.8%), methanol (99.8%), isopropanol (99.8%), polycaprolactone (PCL, MW 80000 g mol -1 ) and doxorubicin hydrochloride (98.0-102.0%) were purchased from Sigma Aldrich. Squalane (SQ, 98%) was purchased from Alfa Aesar. Milli-Q water filtered through 0.22 µm pore size hydrophilic filters (18.2 MΩ-cm) was supplied by a Milli-Q® integral water purification system. NIH/3T3 (CRL-1658), HeLa-WT (CCL-2) and MCF7 (HTB-22) cell lines were purchased from ATCC, dulbecco's modified eagle's medium (DMEM), RPMI 1640 cell culture media (RPMI), fetal bovine serum (FBS), penicillin streptomycin (PS), paraformaldehyde, triton X-100, dimethyl sulfoxide (DMSO), Phalloidin FITC-labelled, DAPI, MTT assay kit and glutamine were purchased from Sigma Aldrich.

Serio F., Silvestri N., Kumar Avugadda S., Nucci G.E.P., Nitti S., Onesto V., Catalano F., D'Amone E., Gigli G., Del Mercato L.L, & Pellegrino T. (2022). Co-loading of doxorubicin and iron oxide nanocubes in polycaprolactone fibers for combining Magneto-Thermal and chemotherapeutic effects on cancer cells. Journal of colloid and interface science, 607(Pt 1).

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Eluate Preparation from Composite Surfaces

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Both the top and bottom surface of the composite specimens were used to prepare eluates of each material tested. Each of these surfaces was scraped separately, ensuring that the entire surface was uniformly removed. Scraping was done mechanically by using a stainless steel dental scaler. Hence, two eluates were prepared from each material for a specific curing time applied: one from the top surface layer and the other from the 4-mm deep bottom surface layer. Samples were pulverized manually with an agate mortar [30] . To obtain the eluate, 0.01 g of pulverized scrapings of a distinct layer was incubated in 1.5 ml of RPMI 1640 cell culture media (Sigma-Aldrich, Taufkirchen, Germany) at 37°C and 5% CO 2 for 48 h. Amount of 0.01 g corresponds to the quantity of scrapings obtained when 1 mm of the top or bottom surface of the specimens was removed. The quantity of medium was empirically determined. The eluates were centrifuged (Biofuge Pico, Heraeus Instruments, Hanau, Germany) at 2000 g for 10 min to remove the scrapings, and the supernatant thus obtained was used for whole blood culture treatment.

Tauböck T.T., Marovic D., Zeljezic D., Steingruber A.D., Attin T, & Tarle Z. (2017). Genotoxic potential of dental bulk-fill resin composites. Dental materials : official publication of the Academy of Dental Materials, 33(7).

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