The HeLa cell line was cultured in Dulbecco's modified Eagle's medium (DMEM) with 1.0 g/L d -glucose (Lonza) supplemented with 2 mM l -glutamine (Sigma-Aldrich) and 10% fetal bovine serum (Biowest). 293T, SH-SY5Y, T98G, LN18, LN229, and U251-MG cell lines were maintained in DMEM with 4.5 g/L d -glucose (Lonza) supplemented with 10% fetal bovine serum. The Flp-In T-REx-293 cell line (Invitrogen) was cultured in DMEM with 4.5 g/L d -glucose supplemented with 10% tetracycline-free fetal bovine serum (Biowest), 100 µg/mL zeocin (Invitrogen) and 15 µg/mL blasticidin S (Invivogen). In stimulation experiments for qRT-PCR the cells were grown in reduced serum medium (0.5% FBS) 24 h prior to stimulation. Cell lines were maintained at 37°C in a humidified atmosphere with 5% CO2. All cell cultures were examined on a regular basis for mycoplasma contamination using PCR (van Kuppeveld et al. 1993 (link)).
Tetracycline free fetal bovine serum
Manufactured by Biowest
Sourced in France
Tetracycline-free fetal bovine serum is a raw material used in cell culture media formulations. It provides essential nutrients and growth factors to support the growth and proliferation of various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Lonza (2 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
D glucose, by Biowest (1 mentions)
Blasticidin s, by InvivoGen (1 mentions)
D glucose, by Lonza (1 mentions)
Flp in t rex 293 cell line, by Thermo Fisher Scientific (1 mentions)
Zeocin, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Merck Group (1 mentions)
Puromycin, by InvivoGen (1 mentions)
2 protocols using tetracycline free fetal bovine serum
1
Cell Culture Protocols for Multiple Cell Lines
2
Maintenance and Characterization of U251-MG Cell Line
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U251-MG cell line was maintained in DMEM with 4.5 g/L D-glucose (Lonza, Walkersville, MD, USA) supplemented with 10% fetal bovine serum. The Sleeping Beauty transposon-based cell lines with doxycycline inducible transgene expression were cultured in media supplemented with 10% tetracycline-free fetal bovine serum (Biowest, Nuaillé, France) and 2 μg/mL puromycin (Invivogen, San Diego, CA, USA). In stimulation experiments for RT-qPCR the cells were grown in reduced serum medium (0.5% FBS) 24 h prior to stimulation. Cell lines were maintained at 37 °C in a humidified atmosphere with 5% CO2. All cell cultures were examined on a regular basis for mycoplasma contamination using PCR [33 (link)].
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