GNP accumulation in cells or organs was quantified using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES). Following 16 h of incubation with GNPs, the cells were washed three times with PBS and the cells were suspended from the monolayer cultures with 0.25% trypsin-EDTA (Ethylenediaminetetraacetic acid) (Gibco, Burlington, ON, Canada) for quantification of GNPs present per cell. Cells were counted with either a hemocytometer (Hausser Scientific, Horsham, UK) or a Vi-CELL XR automated cell counter (Beckman Coulter, Brea, CA, USA). The surgically removed organs were weighed and homogenized. Then, the samples were treated with aqua regia (mixture of 37% hydrochloric acid (HCl) (Sigma-Aldrich) and 70% nitric acid (HNO3) (Caledon Laboratories Ltd., Georgetown, ON, Canada) at a ratio of 3:1 in a silica oil bath. The samples were diluted and concentrations of gold (Au) atoms were measured (mg/L) with the Optima 7300 DV ICP AES (Perkin Elmer, Waltham, MA, USA). The resulting gold atom counts were converted to GNPs per cell or per organ weight.
Optima 7300 dv icp aes
Manufactured by PerkinElmer
Sourced in United States
The Optima 7300 DV ICP AES is a laboratory instrument manufactured by PerkinElmer. It uses inductively coupled plasma atomic emission spectroscopy (ICP-AES) to perform quantitative elemental analysis of samples.
Automatically generated - may contain errors
Lab products found in correlation
Hemocytometer, by Hausser Scientific (2 mentions)
Vi cell xr automated cell counter, by Beckman Coulter (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Jem 2100f microscope, by JEOL (1 mentions)
Sirion 200 microscope, by Thermo Fisher Scientific (1 mentions)
Vario el 3 element analyzer, by Elementar (1 mentions)
Aqua regia, by Merck Group (1 mentions)
Optima 7300 dv, by PerkinElmer (1 mentions)
X pert pro super diffractometer, by Philips (1 mentions)
Escalab 250xi spectrometer, by Thermo Fisher Scientific (1 mentions)
Geminisem 500, by Zeiss (1 mentions)
7 protocols using optima 7300 dv icp aes
1
Quantification of GNP Accumulation
2
Elemental Analysis of ICP-AES Samples
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Elemental analysis of the ICP‐AES samples was performed using the acidic digestion method.[23 ] Briefly, samples were digested in a 2:1 mixture of 70% HNO3 and 30% H2O2 at 85 °C in a hot water bath for 30 min. The digested solutions were diluted (1:5) with distilled water and filtered using 0.22 µm filters (Millex‐GV Syringe Filters, PVDF Durapore Membrane 13 mm diameter, 0.22 µm pore size ethylene oxide sterilized, Millipore, Burlington, MA). The first milliliter of the filtrate was used to saturate the filter, and ≈4 mL of the solution was collected for analysis. The filtrates were analyzed for Mn content using ICP‐AES (Optima7300 DV ICP‐AES, PerkinElmer Ltd, Boston, MA) analysis. The exact tissue weights and dilution volumes were documented for use in final calculations. The concentrations of Mn were measured in triplicate, adjusted to the sample volume and normalized to the brain weight.
3
Comprehensive Characterization of Catalytic Materials
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Transmission electron microscopy (TEM) images were taken on a JEM 2100 F microscope (JEOL, Japan) operated at 200 kV. Scanning electron microscopy (SEM) imaging was carried out on a Sirion 200 microscope (FEI, USA) operated at 5 kV. Nitrogen sorption isotherms were obtained at 77 K with a Micromertics ASAP 3020 analyzer (Micromertics, USA). Before each measurement, the sample was degassed in vacuum at 200 °C for at least 5 h. The Brunauer-Emmett-Teller (BET) method was used to calculate the specific surface areas of the samples. The Barrett-Joyner-Halenda (BJH) model was utilized to analyze pore size distributions, based on which total pore volumes could be obtained. Powder X-ray diffraction (XRD) data were collected with a MiniFlex 600 diffractometer (Rigadu, Japan) using Cu Kα radiation (40 kV, 15 mA). The X-ray photoelectron spectra (XPS) were recorded on an ESCALab MKII X-ray photo-electron spectrometer using Mg Kα radiation as an exciting source. The Co and B contents were determined with an Optima 7300 DV ICP-AES (PerkinElmer, USA). The N and C contents were analyzed with a VarioELIII element analyzer (Elementar Analysensysteme GmbH, Germany).
4
Quantifying Cellular GNP Uptake via ICP-AES
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GNP accumulation in cells was quantified using inductively coupled plasma atomic emission spectroscopy ((ICP-AES) Optima 7300 DV, Perkin Elmer Inc., Waltham, USA). Following sixteen hours of incubation with GNPs, the cells were washed three times with Phosphate Buffer Saline (PBS) and the cells were suspended from the monolayer cultures with 0.25% trypsin-EDTA (Gibco Invitrogen, Carlsbad, California) for quantification of GNPs present per cell. Cells were counted with either a hemocytometer (Hausser Scientific, Horsham, PA, USA) or a Vi-CELL XR automated cell counter (Beckman Coulter, Brea, CA, USA) and then treated with aqua regia (mixture of 25% hydrochloric acid (HCl) (Sigma-Aldrich) and 75% nitric acid (HNO3) (Caledon Laboratories Ltd., Georgetown, Canada) in a ratio of 3:1 v/v) in a silica oil bath. The samples were diluted and concentrations of gold (Au) atoms were measured in (mg/L) with the Optima 7300 DV ICP AES (Perkin Elmer, Waltham, MA, USA). The number of GNPs of each sample was calculated.
5
Comprehensive Physicochemical Analysis of Catalysts
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XRD patterns were obtained by using a Philips X’Pert ProSuper diffractometer with Cu Kα radiation (λ = 1.54178 Å). The morphology was examined by SEM using the ZEISS GeminiSEM 500. TEM and HRTEM images were undertaken on a JEM-2100F field-emission electron microscope with an accelerating voltage of 200 kV. HAADF-STEM was performed on a JEOL JEM-ARF200F HRTEM with a spherical aberration corrector at voltage of 200 kV. EDS elemental mappings were obtained on JEOL JEM-F200 instrument. All catalysts for synthesis were dissolved in aqua regia and obtain the contents of Fe, Co, Ni and Cr in as-prepared catalysts were determined by ICP-AES on a PerkinElmer Optima 7300 DV ICP-AES instrument. The XPS were recorded on a Thermo ESCALAB 250Xi spectrometer with an excitation source of monochromatized Al Ka (hν = 1486.6 eV) and a pass energy of 30 eV. The values of binding energies were calibrated with the C 1 s peak of contaminant carbon at 284.80 eV.
6
Quantifying Ionic Concentrations in Nucleic Acids
7
Sequential Extraction Protocol for Soil Analysis
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Add the next extracting solution in the sequence (see table 2) and repeat steps 2 to 5.
For the extractions with 0.1, 0.5, 1.0 and 5 M acid extracts, carry out steps 2-5 with the addition 0.25, 0.50, 0.75 and 1 ml, respectively, of 9% (v/v) H 2 O 2 prior to making up the final acid volume to 10 ml (as shown in Table 2).
The hydrogen peroxide is added to aid the digestion of the organic material and Mn oxides as the sequential extraction proceeds.
The tubes are weighed before and after removal of the supernatant solution to determine the volume of solution withdrawn so that the volume of solution in contact with the soil during shaking is known. A small amount of solution is left behind (ca. 0.1-0.3ml) but this is taken into account by the self-modelling mixture resolution data processing which is carried out after the analysis of extracts.
The extracted solutions were analysed for major and trace elements required for the CISED data processing using a Perkin Elmer Optima 7300DV ICP-AES. The sample introduction system was a Conikal U-Series concentric glass nebulizer with a glass cyclonic spray chamber. The ICP-AES operating conditions and wavelengths used are given in tables S1 and S2 in the supplementary information. The wavelengths were chosen to give suitable detection limits, linear ranges and freedom from spectral interference in the CISED extraction matrix.
For the extractions with 0.1, 0.5, 1.0 and 5 M acid extracts, carry out steps 2-5 with the addition 0.25, 0.50, 0.75 and 1 ml, respectively, of 9% (v/v) H 2 O 2 prior to making up the final acid volume to 10 ml (as shown in Table 2).
The hydrogen peroxide is added to aid the digestion of the organic material and Mn oxides as the sequential extraction proceeds.
The tubes are weighed before and after removal of the supernatant solution to determine the volume of solution withdrawn so that the volume of solution in contact with the soil during shaking is known. A small amount of solution is left behind (ca. 0.1-0.3ml) but this is taken into account by the self-modelling mixture resolution data processing which is carried out after the analysis of extracts.
The extracted solutions were analysed for major and trace elements required for the CISED data processing using a Perkin Elmer Optima 7300DV ICP-AES. The sample introduction system was a Conikal U-Series concentric glass nebulizer with a glass cyclonic spray chamber. The ICP-AES operating conditions and wavelengths used are given in tables S1 and S2 in the supplementary information. The wavelengths were chosen to give suitable detection limits, linear ranges and freedom from spectral interference in the CISED extraction matrix.
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