Gentamycin

GSCs derived from high-grade glioma samples, the MES subtype (GSC-83, GSC-326) and the PN subtype (GSC-19, GSC-84), were described by us previously^{3 (link)}. The GSCs were cultured in suspension in GSC growth medium [DMEM-F12 (Cat# 10565, Life Technologies) supplemented with B27 (1:50), heparin (5 mg/mL), basic FGF (bFGF) (20 ng/mL), and EGF (20 ng/mL)] in 100 × 20 mm Petri dishes (Cat# 0875711Z, Fisher Scientific) at 37 °C with 5% CO₂. Growth medium was changed every 3 or 4 days. U87MG cells (Cat# HTB-14, ATCC) were cultured in EMEM (Cat#11095080, Thermo Fisher Scientific) with 10% FBS(HI), Sodium Pyruvate (1 mM) (Cat#11360-070, Invitrogen), MEM supplemented with Non-Essential Amino acids (0.1 mM) (Cat#11140050, Thermo Fisher Scientific), antibiotic/antimycotic (4 ml) (Cat#15240062, Thermo Fisher Scientific) and Gentamycin (5 µg/ml) (Cat# 9354, Irvine Scientific). Growth medium was changed every 3 or 4 days. 293-FT cells (Cat# R70007, Thermo Fisher Scientific) were cultured in DMEM (Cat# 45000-304, VWR) with 10% FBS(HI), Glutamine (2 mM) (Cat# 25030081, Thermo Fisher Scientific) and antibiotic/antimycotic (4 ml) (Cat# 15240062, Thermo Fisher Scientific). All cells were cultured at 37 °C with 5% CO₂. Details for each cell line used herein are in Supplementary Table 1.