Mouse anti cd90 pecytm7

The density of second-generation BMSCs was adjusted to 2 × 10⁷ cells/mL. The control group received 50 µL of buffer, while the single-label group received 5 µL of hamster anti-CD29-AF647 (BD Biosciences, Franklin Lakes, NJ, USA), mouse anti-CD90-PECyTM7 (BD Biosciences), mouse anti-CD106-PE (BD Biosciences), mouse anti-CD11b-V450 (BD Biosciences), or mouse anti-CD45-FITC (BD Biosciences) to each branch-flow sampling tube, after which 45 µL of buffer was added to each tube. The multicolor group received 5 µL of each antibody into a one-branch-flow sampling tube, and then, 25 µL of buffer was added. Next, 50 µL of cell suspension was added to each flow tube, the tubes were incubated at room temperature for 30 min and washed twice with staining buffer, and then, 500 µL of buffer was added to each tube for detection by flow cytometry (Beckman Coulter Life Sciences, Brea, CA, USA).

The density of passage 3 BMSCs was adjusted to 2 × 10⁷ cells/mL, and 50 µL of cell suspension was used per sample. Staining buffer was used in the control group. Cells were incubated with 5 µL mouse anti-CD34-FITC (BD, USA), mouse anti-CD45-FITC (BD, USA), mouse anti-CD45-FITC (BD, USA), mouse anti-CD90-PECyTM7 (BD, USA), or mouse anti-CD105-PE (BD, USA). Then, 45 µL staining buffer was added to each tube, followed by mixing, and 5 µL of each antibody was put into the flow detection tube. Then, 30 µL staining buffer was added. Next, 50 µL of cell suspension was added to each tube, samples were mixed, incubated at 4 °C in the dark for 30 min, and washed three times with staining buffer, and then 500 µL staining buffer was added to each tube. Surface antigens were detected by flow cytometry (Beckman, USA).