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Water 2707

Manufactured by Waters Corporation
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The Waters 2707 is a liquid chromatography autosampler designed for high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) applications. The autosampler accurately and precisely injects liquid samples into the chromatographic system for analysis.

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Lab products found in correlation

515 hplc pump, by Waters Corporation (4 mentions) Kintex c18 hplc column, by Phenomenex (2 mentions) Empower software, by Waters Corporation (2 mentions)

Spelling variants of this product

Waters 2707 autosampler (20 citations) Waters 2707 (12 citations) Waters 1515/2414/2707 (2 citations) Waters 2707 autosampler delivery system (2 citations)

4 protocols using water 2707

1

Electrochemical Quantification of Biogenic Amines

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Biogenic amine concentrations were determined in the Vanderbilt University Medical Center Hormone Assay & Analytical Core utilizing an Antec Decade II (oxidation: 0.65) electrochemical detector operated at 33 °C. Twenty microliter samples of the supernatant were injected using a Water 2707 autosampler onto a Phenomenex Kintex C18 HPLC column (100 × 4.60 mm, 2.6um). Biogenic amines were eluted with a mobile phase consisting of 89.5% 0.1 M TCA, 10−2 M sodium acetate, 10−4 M EDTA and 10.5% methanol (pH 3.8). Solvent was delivered at 0.6 ml/min using a Waters 515 HPLC pump. HPLC control and data acquisition were managed by Empower software. Isoproterenol (5 ng/mL) was included in the homogenization buffer for use as a standard to quantify the biogenic amines.
Naguib S., Bernardo-Colón A., Cencer C., Gandra N, & Rex T.S. (2019). Galantamine protects against synaptic, axonal, and vision deficits in experimental neurotrauma. Neurobiology of disease, 134, 104695.
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2

HPLC Quantification of Biogenic Amines

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Biogenic amine concentrations were determined utilizing an Antec
Decade II (oxidation: 0.65) electrochemical detector operated at
33°C. Twenty microliter samples of the supernatant were injected
using a Water 2707 autosampler onto a Phenomenex Kintex C18 HPLC column
(100 × 4.60 mm, 2.6 μm). Biogenic amines are eluted with a
mobile phase consisting of 89.5% 0.1M TCA, 10−2 M
sodium acetate, 10−4 M EDTA and 10.5% methanol (pH
3.8). Solvent was delivered at 0.6 ml/min using a Waters 515 HPLC pump.
Using this HPLC solvent the following biogenic amines were eluted in the
following order: Norepinephrine, Epinephrine, DOPAC, Dopamine, 5-HIAA,
HVA, 5-HT, and 3-MT. Data acquisition was managed by Empower software
(Waters Corporation, Milford, MA USA). Isoproterenol (5 ng/mL) was
included in the homogenization buffer for use as an internal standard to
quantify the biogenic amines of interest.
Wu J., Kung J., Dong J., Chang L., Xie C., Habib A., Hawes S., Yang N., Chen V., Liu Z., Evans R., Liang B., Sun L., Ding J., Yu J., Saez-Atienzar S., Tang B., Khaliq Z., Lin D.T., Le W, & Cai H. (2019). Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning. Cell reports, 28(5), 1167-1181.e7.
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3

Biogenic Amine Analysis by HPLC-ECD

Cited 1 time
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Biogenic amines were analyzed in the Vanderbilt University Neurochemistry Core as described previously8 (link). Briefly, biogenic amine concentrations were determined utilizing an Antec Decade II (oxidation: 0.65) electrochemical detector operated at 33 °C. 20μl samples of the supernatant were injected using a Water 2707 autosampler onto a Phenomenex Kintex C18 HPLC column (100 × 4.60 mm, 2.6 μm). Biogenic amines are eluted with a mobile phase consisting of 89.5% 0.1 M TCA, 10-2 M sodium acetate, 10-4 M EDTA and 10.5 % methanol (pH 3.8). Solvent was delivered at 0.6 ml/min using a Waters 515 HPLC pump. Using this HPLC solvent the following biogenic amines were eluted in the following order: Norepinephrine, Epinephrine, DOPAC, Dopamine, 5-HIAA, HVA, 5-HT, and 3-MT. Data acquisition was managed by Empower software (Waters Corporation, Milford, MA USA). Isoproterenol (5 ng/mL) was included in the homogenization buffer for use as an internal standard to quantify the biogenic amines of interest.
Liu Z., Yang N., Dong J., Tian W., Chang L., Ma J., Guo J., Tan J., Dong A., He K., Zhou J., Cinar R., Wu J., Salinas A.G., Sun L., Kumar M., Sullivan B.T., Oldham B.B., Pitz V., Makarious M.B., Ding J., Kung J., Xie C., Hawes S.L., Wang L., Wang T., Chan P., Zhang Z., Le W., Chen S., Lovinger D.M., Blauwendraat C., Singleton A.B., Cui G., Li Y., Cai H, & Tang B. (2022). Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction. Nature Communications, 13, 3490.
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4

Electrochemical Quantification of Biogenic Amines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biogenic amine concentrations were determined in the Vanderbilt University Medical Center Hormone Assay & Analytical Core utilizing an Antec Decade II (oxidation: 0.65) electrochemical detector operated at 33 °C. Twenty microliter samples of the supernatant were injected using a Water 2707 autosampler onto a Phenomenex Kintex C18 HPLC column (100 × 4.60 mm, 2.6um). Biogenic amines were eluted with a mobile phase consisting of 89.5% 0.1 M TCA, 10−2 M sodium acetate, 10−4 M EDTA and 10.5% methanol (pH 3.8). Solvent was delivered at 0.6 ml/min using a Waters 515 HPLC pump. HPLC control and data acquisition were managed by Empower software. Isoproterenol (5 ng/mL) was included in the homogenization buffer for use as a standard to quantify the biogenic amines.
Naguib S., Bernardo-Colón A., Cencer C., Gandra N, & Rex T.S. (2019). Galantamine protects against synaptic, axonal, and vision deficits in experimental neurotrauma. Neurobiology of disease, 134, 104695.
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