Ab230537

Manufactured by Abcam

Ab230537 is a laboratory equipment product. It is a specialized tool used in scientific research and experimentation. The core function of this product is to perform a specific task related to the research and analysis process. However, a detailed description of its intended use or application cannot be provided in an unbiased and factual manner without the risk of extrapolation or interpretation.

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Lab products found in correlation

Propofol, by Merck Group (1 mentions) Ab128251, by Abcam (1 mentions) Ab178846, by Abcam (1 mentions) Ab11268, by Abcam (1 mentions) Ab30559, by Abcam (1 mentions) Ab1543p, by Abcam (1 mentions) Ab5392, by Merck Group (1 mentions) Ab16997, by Abcam (1 mentions)

2 protocols using ab230537

Propofol Modulates Neuronal Excitability

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Propofol (2,6-diisopropylphenol) was obtained from Sigma-Aldrich (United States). For in vivo experiments, Propofol was used at a dose of 50 mg/kg and for in vitro experiments Propofol from 5–80 μM concentrations were used. The antibodies used in this study were Anti-CACNA1H (Cav3.2, ab128251, and Abcam), Anti-Connexin 43/GJA1 (phospho S368, ab30559, Abcam), Anti-Connexin 43/GJA1 antibody (ab230537, Abcam), Anti-Iba1 antibody (ab178846, Abcam), Gap27 (HY-P0139, MedChemExpress), and Map2 (ab11268, Abcam).

Zhang T., Wang Y., Xia Q., Tu Z., Sun J., Jing Q., Chen P, & Zhao X. (2021). Propofol Mediated Protection of the Brain From Ischemia/Reperfusion Injury Through the Regulation of Microglial Connexin 43. Frontiers in Cell and Developmental Biology, 9, 637233.

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Immunostaining of Neuronal Cultures

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Cultures neurons were fixed at D28 of neuronal differentiation in 4% paraformaldehyde +5% sucrose in DPBS for 20 min at room temperature. Cells were incubated with blocking buffer containing 4% horse serum, 0.1M Glycine, and 0.3% Triton-X in PBS for 1 h at room temperature. Primary antibodies, diluted in 4% horse serum in PBS, were incubated overnight at 4°C. Secondary antibodies were diluted in 4% horse serum in PBS and applied for 1 h at room temperature. Samples were washed 3x with PBS and imaged on spinning disc confocal microscope (Andor Dragonfly) with a 20x air interface objective using Fusion software. The following antibodies were used: chicken anti-MAP2 (1:10,000, Abcam ab5392), Rabbit anti-Syn-1 (1:1000 millipore AB1543P), Rabbit anti-GFAP (1:250, Abcam ab16997), Rabbit anti-Cx43 (1:500, ab230537). Goat Alexafluor plus-555- conjugated anti-mouse (Cat # A32727) and Goat anti-Rabbit Alexafluor plus-488 (Cat # A32731).

, & Igloi G.L. (1998). Variability in the stability of DNA–peptide nucleic acid (PNA) single-base mismatched duplexes: Real-time hybridization during affinity electrophoresis in PNA-containing gels. Proceedings of the National Academy of Sciences of the United States of America, 95(15), 8562-8567.

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