Cellular lysis was performed using a lysis buffer (1.5 mmol/L EDTA, 50 mmol/L Hepes pH 7.4, 150 mmol/L NaCl, 10% (v/v) glycerol, and 1% (v/v) NP40). Total cellular lysates were loaded onto a 4% to 20% SDS-PAGE gel (Bio-Rad), transferred onto nitrocellulose membrane, and revealed with different antibodies such as homemade anti-proteasome36 (link) and anti-HNE 4-hydroxy-nonenal37 (link). Commercial antibodies used were, MitoProfile® Total OxPhOS WB Antibody Cocktail and anti-phospho-gamma H2AX-Ser139 (Thermofischer, Saint Aubin, France), anti-actin and anti-NRF2 (Abcam, Paris, France) and anti-human PARP (BD Biosciences, Le Pont de Claix, France). Direct recording of the chemi-luminescence (GeneGnome Syngene) and quantification (GeneSnap software) were performed (Ozyme, St Quentin en Yvelines, France).
Anti human parp
Manufactured by BD
Sourced in France
Anti-human PARP is a laboratory reagent that specifically binds to and detects the presence of the poly(ADP-ribose) polymerase (PARP) protein in human samples. PARP is an enzyme involved in various cellular processes, including DNA repair and programmed cell death. This product can be used to identify and quantify PARP levels in research applications.
Automatically generated - may contain errors
2 protocols using anti human parp
1
Cellular lysis and Western blot analysis
2
Protein Extraction and Immunoblotting
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Protein extraction and immunoblotting analysis were performed as previously described by Velaithan et al. 23 Primary antibodies used were anti-MSH2, anti-MLH1, and antihuman PARP (BD Pharmingen, Franklin Lakes, NJ); antiγ-H2A.X (S139), anti-cdc2, anti-p-cdc2 (Y15), anti-p-cdc2 (T14), anti-p-cdc2 (T161), anti-cleaved caspase 3, and anti-p21 Waf1/Cip1 (Cell Signaling, MA); anti-β-actin (Merck Millipore, Palo Alto, CA); and anti-p53 (Novocastra, NE, UK). anti-β-actin monoclonal antibody was used to normalize loading.
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