Anti rnf126

For WB, the following conditions are used. Anti-RNF126 (Clone C-1; 1:200; Santa Cruz Technology); Anti-RNF126 (Clone 1B5; 1:1000; Abcam); Anti-BRCA1 (Clone D-9; 1:200; Santa Cruz Technology); Anti-RAD51 (Clone H92; 1:200; Santa Cruz Technology); Anti-BRCA2 (clone 5.23; 1:500; EMD Millipore); Anti-RAD52 (Clone 5H9; 1:200; GeneTex); Anti-RPA1 (Clone NA13; 1:100; Calbiochem/EMD Millipore) and anti-RPA2 (Clone NA18; 1:100; Calbiochem/EMD Millipore); Anti-53BP1(Clone 1B9; 1:1000; Novus biologicals); Anti-FLAG M2 (Clone M2; 1:1000; Sigma-Aldrich); Anti-E2F1(Clone KH95; 1:200; Santa Cruz Technology); Anti-HA (Clone 16B12; 1:1000; Covance); Anti-His (Clone H-15; 1:200; Santa Cruz Technology); Anti-GST (Clone B-14; 1:200; Santa Cruz Technology); Anti-Filamin (Clone FLMN01; 1:1000; Pierce); Anti-β-Actin ( Clone AC-74; 1:10000; Sigma-Aldrich). Secondary antibodies were goat-anti-mouse IgG–HRP conjugated and goat-anti-rabbit IgG–HRP conjugated both at 1:5 000 dilutions for immune blotting.

To detect proteins, antibodies were used as follows: anti-RNF126 (Santa Cruz Biotechnology, Dallas TX USA, clone C-1, 1:200); anti-14–3-3σ (Santa Cruz Biotechnology, clone C-18, 1:200); anti-FLAG (Sigma-Aldrich, clone M2, 1:1000); anti-GST (Santa Cruz Biotechnology, 1–109, 1:200), anti-β-actin (Sigma-Aldrich, clone AC-74, 1:50000); anti-His (Santa Cruz Biotechnology, Clone H-15, 1:200); and anti-phospho-histone H3-Ser10 (Cell Signaling Technology, #3377S). Cell Signaling Technology’s goat anti-mouse IgG–horseradish peroxidase (HRP) (#7076S, 1:1000) and goat anti-rabbit IgG-HRP (#7074S, 1:1000) were subsequently used as secondary antibodies. The primary antibodies used for immunofluorescence were anti-CDK1 (Abcam, ab133327, 1:100), and anti-cyclin B1 (Santa Cruz Biotechnology, sc-245, 1:100). Thermo Fisher Scientific’s goat anti-mouse IgG (H+L) Alexa Fluor 594 (A-11032, 1:400), and chicken anti-rabbit IgG (H+L) Alexa Fluor 488 (A-21441, 1:400) were used as secondary antibodies.