Log In Sign up
The largest database of trusted experimental protocols

B6 cg tg prrx1 cre 1cjt j

Manufactured by Jackson ImmunoResearch
Visit Supplier
Sourced in Montenegro, United States

B6.Cg-Tg(Prrx1-cre)1Cjt/J is a transgenic mouse strain that expresses Cre recombinase under the control of the Prrx1 promoter. The Prrx1 gene is expressed in mesenchymal progenitor cells, which can contribute to various cell lineages during development.

Automatically generated - may contain errors

Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (4 mentions) B6 cg fbn1tsk j, by Jackson ImmunoResearch (1 mentions) B6 cg gt rosa 26sortm9 cag tdtomato hze j, by Jackson ImmunoResearch (1 mentions) B6.129s tnftm1gkl j, by Jackson ImmunoResearch (1 mentions) B6.129s2 il6tm1kopf j, by Jackson ImmunoResearch (1 mentions) B6.129p2 lyz2tm1 cre ifo j, by Jackson ImmunoResearch (1 mentions) B6.129s4 tet1tm1 1jae j, by Jackson ImmunoResearch (1 mentions) Lm 485, by Inotiv (1 mentions) Gata4tm1.1sad j, by Jackson ImmunoResearch (1 mentions) B6 cg tg, by Jackson ImmunoResearch (1 mentions) B6 cg gt, by Jackson ImmunoResearch (1 mentions) 26sortm9, by Jackson ImmunoResearch (1 mentions) Hze j, by Jackson ImmunoResearch (1 mentions)

7 protocols using b6 cg tg prrx1 cre 1cjt j

1

Genetic Mouse Models for Tissue-Specific Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female C57BL/6J, B6.Cg-Fbn1Tsk/J, B6.Cg-Tg(Prrx1-cre)1Cjt/J, B6.Cg-Tg (Sp7-tTA, tetO-EGFP/cre)1Amc/J, and B6.129S4-Mtortm1.2koz/J mice were purchased from The Jackson Laboratory and maintained in C57BL/6J background in at least 10 backcrosses. Age-matched female littermates were used in all experiments. Female immunocompromised nude mice (Beige nu/nu XIDIII) were purchased from Harlan. IL4Rα null (Il4rα−/−) and floxed (Il4rαϕ/ϕ; Herbert et al., 2004 (link)) mice were gifts from F. Brombacher (University of Cape Town, Cape Town, South Africa). To generate tissue-specific Cre-mediated KO models, Cre-, floxed-, and Fbn1+/− mice were intercrossed, and age-matched female littermates were used as WT controls. All animal experiments were performed under the institutionally approved protocols for the use of animal research (University of Southern California protocol numbers 11141, 11953, and 11327).
Chen C., Akiyama K., Wang D., Xu X., Li B., Moshaverinia A., Brombacher F., Sun L, & Shi S. (2015). mTOR inhibition rescues osteopenia in mice with systemic sclerosis. The Journal of Experimental Medicine, 212(1), 73-91.
+ Open protocol
+ Expand
2

Genetically Modified Mouse Models

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female C57BL/6J (WT, No. 000664), B6.129S-Tnftm1Gkl/J (TNFα−/−, No. 003008), B6.129S-Tnfrsf1atm1ImxTnfrsf1btm1Imx/J (TNFR−/−, No. 003243), B6.129S7-Ifngtm1Ts/J (IFNγ−/−, No. 002287), B6.129S2-Il6tm1Kopf/J (IL-6−/−, No. 002650), B6.Cg-Tg(Prrx1-cre)1Cjt/J (Prx1-Cre, No. 005584), and B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (tdTomato, No. 007909) mice were purchased from Jackson Laboratory and maintained on a C57BL/6J background for at least ten backcrosses. Prx1-Cre and tdTomato transgenics were interbred to obtain Prx1-Cre;tdTomato mice for tracking mesenchymal cells in vivo. Genotyping was performed by PCR using tail samples from mice and primer sequences provided by Jackson Laboratory. Age-matched female littermates were used in all experiments. Female immunocompromised nude mice (Beige nu/nu XIDIII) were purchased from Harlan. Mice were housed under pathogen-free conditions, maintained on a standard 12-h light-dark cycle, and given food and water ad libitum. All animal experiments were performed under institutionally approved protocols for animal research (University of Pennsylvania, Protocol No. 805478).
Yu W., Chen C., Kou X., Sui B., Yu T., Liu D., Wang R., Wang J, & Shi S. (2021). Mechanical force-driven TNFα endocytosis governs stem cell homeostasis. Bone Research, 8, 44.
+ Open protocol
+ Expand
3

Conditional Deletion of Adamts9 in Mouse Germline and Limbs

Check if the same lab product or an alternative is used in the 5 most similar protocols
For deletion in the male germline, Prm1-Cre mice (B6Ei.129S4-Tg(Prm1-cre)58Og/EiJ; Jackson Laboratories, Bar Harbor, ME) were crossed to Adamts9fl-neo/+ mice. Male mice carrying both transgenes were selected by PCR genotyping (see below). These were bred to wild-type C57BL/6 mice to select mice bearing the deleted allele (Adamts9del/+) but not Prm1-Cre. Successful excision of the neo cassette was also verified by sequencing PCR products. Adamts9del/+ mice were subsequently intercrossed and the vaginal plug was used to determine potential fertilization (The morning the plug was observed was designated as E0.5). Pregnant dams were sacrificed at E7.5, E8.5, or E9.5. These embryos and live born progeny were genotyped to ascertain survival of Adamts9del/del progeny. For limb-specific conditional deletion, Prx1-Cre mice (B6.Cg-Tg(Prrx1-cre)1Cjt/J, Jackson Laboratories, Bar Harbor, ME) were crossed to Adamts9fl/+ mice. For conditional deletion, male Prx1-Cre; Adamts9fl/+ or Prx1-Cre; Adamts9fl/fl mice were bred to Adamts9fl/fl or Adamts9del/+ mice respectively to obtain mice with conditional deletion (Prx1-Cre; Adamts9fl/fl or Prx1-Cre; Adamts9fl/del) in limb mesoderm.
Dubail J., Aramaki-Hattori N., Bader H.L., Nelson C.M., Katebi N., Matuska B., Olsen B.R, & Apte S.S. (2014). A new Adamts9 conditional mouse allele identifies its non-redundant role in interdigital web regression. Genesis (New York, N.Y. : 2000), 52(7), 702-712.
+ Open protocol
+ Expand
4

Nrp2 Knockout Mice Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nrp2 heterozygous and Nrp2lox/lox mice on C57Bl/6 background were kindly provided by D. Ginty (The Johns Hopkins University School of Medicine, Baltimore, MD) and were maintained in a conventional animal facility (18 (link)). B6.Cg-Tg(Prrx1-cre)1Cjt/J and B6.129P2-Lyz2tm1(cre)Ifo/J mice were obtained from the Jackson Laboratory. Mice with a conditional deletion of Nrp2 in osteoblast precursors and mature osteoblasts were generated by crossing paired related homeobox 1 (Prrx1)-Cre mice with Nrp2lox/lox mice, yielding osteoblast-specific Nrp2 knockdown mice (Nrp2Ob- mice). Mice with a conditional deletion of Nrp2 in osteoclast precursors and mature osteoclasts were generated by crossing Lysozyme 2 (Lyz2)-Cre mice with Nrp2lox/lox mice, yielding osteoclast-specific Nrp2 knockdown mice (Nrp2Oc- mice). Genotyping was performed by polymerase chain reaction (PCR) on genomic DNA from toe clips obtained from preweanling mice. All animal experiments were approved by the ethical committee of the KU Leuven (P090/2019).
Verlinden L., Doms S., Janssens I., Meyer M.B., Pike J.W., Carmeliet G, & Verstuyf A. (2023). Neuropilin 2 in osteoblasts regulates trabecular bone mass in male mice. Frontiers in Endocrinology, 14, 1223021.
+ Open protocol
+ Expand
5

Mouse Models for Epigenetic Regulation Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female C57BL/6J (JAX #000664), B6.129S4-Tet1tm1.1Jae/J (JAX #017358, Tet1+/−), B6.129S-Tet2tm1.1laai/J (JAX #017573, Tet2fl/fl), and B6.Cg-Tg (Prrx1-cre)1Cjt/J (JAX #005584, Prx1cre) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Age-matched littermates were used in all experiments. The 8–10-week-old C57BL/6J mice were ovariectomized (OVX)22 (link), and sham-operated age-matched female mice served as controls. We purchased immunocompromised nude mice (Beige nu.nu XIDIII) from Harlan (Indianapolis, IN, USA). To generate Tet1−/−Prx1creTet2fl/fl mice, we mated Tet1+/−Prx1creTet2fl/+ mice with Tet1+/−Tet2fl/+ mice, and littermates whose genetic status was Prx1cre were used as controls. All animal experiments were performed under institutionally approved protocols for the use of animal research (University of Pennsylvania Institutional Animal Care and Use Committee #805478).
Yang R., Yu T., Kou X., Gao X., Chen C., Liu D., Zhou Y, & Shi S. (2018). Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter. Nature Communications, 9, 2143.
+ Open protocol
+ Expand
6

GATA4 Conditional Knockout Mouse

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal experiments were approved by the Institutional Animal Care and Use Committee at the University of Tennessee Health Science Center. Animals were maintained in a specific pathogen free environment at 20–26°C with a relative humidity of 30–70% and a 12 h light/dark cycle. Commercial rodent chow (LM-485, Teklad, Madison, WI) and drinking water were available ad libitum. GATA4fl/fl mice were purchased from Jackson Laboratory (Gata4tm1.1Sad/J, JAX stock #008194). The floxed homozygous mice (GATA4fl/fl) were backcrossed for 10 generations to FVB background and then to C57BL/6 for 10 generations. The C57BL/6 GATA4fl/fl mice were crossed to B6.Cg-Tg(Prrx1-cre)1Cjt/J (JAX stock #005584) to produce GATA4fl/fl/Cre+ (Prx-cKO) and GATA4fl/fl/Cre− littermate control mice (referred to as WT mice in the text and figures).
GATA4-Flag-biotin mice (Flag-bio, Gt(ROSA)26Sortm1(birA)Mejr Gata4tm3.1Wtp/J)[11 (link)] were obtained from Jackson Labs (stock #018121).
WNT10B knockout mice (WNT10BKO, WNT10B−/−) were previously described [12 (link)].
Khalid A.B., Pence J., Suthon S., Lin J., Miranda-Carboni G.A, & Krum S.A. (2020). GATA4 Regulates Mesenchymal Stem Cells via Direct Transcriptional Regulation of the WNT Signalosome. Bone, 144, 115819.
+ Open protocol
+ Expand
7

Diabetic Fracture Healing in Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All animal experiments were initiated on 8- to 10-week-old male and female mice conforming to a protocol approved by the University of Pennsylvania Institutional Animal Care and Use Committee. The following mice were purchased from The Jackson Laboratory: C57BL/6J, B6.Cg-Tg(Prrx1-cre)1Cjt/J (Prx1Cre), B6.Cg-Tg(Prrx1-cre/ERT2,-EGFP)1Smkm/J (Prx1CreER), B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (R26TdTomato), and B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J (R26DTA). B6;SJL-Tg(Col2α1-cre)1Bhr/J mice (Col2α1Cre) were obtained from Dr. Patrick O’Connor (Rutgers University, Newark, NJ), and floxed Ikkβ mice (Ikkβf/f) from Dr. Michael Karin (University of California San Diego, La Jolla, CA). Low-dose streptozotocin injection was performed intraperitoneally for T1D induction (5 consecutive days, 50 mg/kg) (Cayman Chemical) as previously described (22 (link)). Animals were considered diabetic when blood glucose was >220 mg/dL 1 week after last injection and remained diabetic for 4–6 weeks prior to fracture surgery.
Ko K.I., Syverson A.L., Kralik R.M., Choi J., DerGarabedian B.P., Chen C, & Graves D.T. (2019). Diabetes-Induced NF-κB Dysregulation in Skeletal Stem Cells Prevents Resolution of Inflammation. Diabetes, 68(11), 2095-2106.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!