The largest database of trusted experimental protocols

Primary rabbit monoclonal anti gapdh antibody

Manufactured by Cell Signaling Technology

The primary rabbit monoclonal anti-GAPDH antibody is a laboratory reagent used for the detection and quantification of the GAPDH protein in biological samples. GAPDH is a commonly used housekeeping gene and its protein is involved in the glycolytic pathway.

Automatically generated - may contain errors

2 protocols using primary rabbit monoclonal anti gapdh antibody

1

Antibody Characterization and Pull-Down Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies of mouse IgG1 monclonal anti-FLAG [Clone number: M2] (# ab49763), rabbit monoclonal anti-CDK1 antibody [Clone number: EPR165] (# ab133327), and rabbit monoclonal anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [Clone number: EPR17621] (# ab183554) were purchased from Abcam. The primary rabbit monoclonal anti-GAPDH antibody [Clone number: D16H11] (# 8884) was purchased from Cell Signaling Technology (CST). Primary mouse monoclonal anti-Green Fluorescent Protein (GFP) antibody [Clone number: GSN149] (# G1546) was purchased from Sigma. The secondary horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (heavy and light chain) antibody (# 7074) and the HRP-conjugated goat anti-mouse IgG (heavy and light chain) antibody (# 7076) were purchased from CST.
The Anti-DDDDK-tag mAb-Magnetic Agarose (M185-10) and anti-GFP magnetic beads Anti-GFP mAb-Magnetic Beads (D153-11) used for pull-down assay were ordered from Medical and Biological Laboratories (MLB).
+ Open protocol
+ Expand
2

Antibody Characterization and Pull-Down Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies of mouse IgG1 monclonal anti-FLAG [Clone number: M2] (# ab49763), rabbit monoclonal anti-CDK1 antibody [Clone number: EPR165] (# ab133327), and rabbit monoclonal anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [Clone number: EPR17621] (# ab183554) were purchased from Abcam. The primary rabbit monoclonal anti-GAPDH antibody [Clone number: D16H11] (# 8884) was purchased from Cell Signaling Technology (CST). Primary mouse monoclonal anti-Green Fluorescent Protein (GFP) antibody [Clone number: GSN149] (# G1546) was purchased from Sigma. The secondary horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (heavy and light chain) antibody (# 7074) and the HRP-conjugated goat anti-mouse IgG (heavy and light chain) antibody (# 7076) were purchased from CST.
The Anti-DDDDK-tag mAb-Magnetic Agarose (M185-10) and anti-GFP magnetic beads Anti-GFP mAb-Magnetic Beads (D153-11) used for pull-down assay were ordered from Medical and Biological Laboratories (MLB).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!