Cell apoptosis was measured by TransDetect® Annexin V-FITC/PI Cell Apoptosis Detection Kit (Transgen, FA101, China). After treatment with various concentrations of IFN-λ1 for 24 h and infected by S. aureus, keratinocytes (2 × 105 cells) were harvested, resuspended in 100 μl one x Annexin V binding buffer and incubated with 5 μl Annexin V-FITC and 5 μl propidium iodide (PI) for 15 min in the dark at room temperature. The samples were detected by a Beckman Coulter FC500 cytometer immediately, and the FlowJo software version 10.4 (BD, United States) was used for data analysis.
Fc500 cytometer
Manufactured by BD
Sourced in United States
The FC500 cytometer is a flow cytometry instrument designed for cell analysis. It provides accurate measurements of various cellular properties, including size, granularity, and fluorescence intensity. The FC500 is capable of analyzing multiple parameters simultaneously, enabling researchers to obtain comprehensive data on cell populations.
Automatically generated - may contain errors
2 protocols using fc500 cytometer
1
Annexin V-FITC/PI Apoptosis Assay
2
Quantifying Murine Cytokines via Flow Cytometry
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Murine cytokines were quantified by bead-based immunoassays adapted on flow cytometry, using the mouse Th1/Th2 10-plex FlowCytomixTM Kit (eBioscience, San Diego, CA, USA) according to the manufacturer’s instructions, using a filter plate and a vacuum filtration system for washing steps. Data were collected on a FC500® cytometer (BD Biosciences, San Jose, USA), and analyzed with FlowCytomix® Pro 3.0 Software (eBioscience). Standard curves were determined for each cytokine from a range of 27–20000 pg/ml. Serum concentrations were expressed in mean fluorescence intensity (MFI).
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