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Anti mouse and anti rabbit secondary antibodies conjugated with horseradish peroxidase hrp

Manufactured by Cell Signaling Technology

Anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP) are laboratory reagents used to detect and quantify target proteins in various immunoassays, such as Western blotting and ELISA. These antibodies bind to the primary antibodies that recognize the target proteins and provide a colorimetric or chemiluminescent signal when exposed to a suitable substrate, enabling the visualization and quantification of the target proteins.

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2 protocols using anti mouse and anti rabbit secondary antibodies conjugated with horseradish peroxidase hrp

1

Antibodies for Pluripotency Markers

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Mouse monoclonal antibodies to p70 S6 kinase (H-9) (SK1-hedles), PABP (10E10), eIF4A (N-19), rabbit polyclonal antibodies to eIF4G-(H-300), eIF4E (FL-217), and goat polyclonal antibodies to eIF3n and TIAR were all purchased from Santa Cruz; whereas the Mouse monoclonal antibodies to G3BP were purchased from BD Biosciences. Mouse monoclonal antibodies to detect the pluripotent markers, SOX2, NANOG, and LIN28A were from Cell Signaling; whereas the KLF4 and DPPA5 were from Abcam and R&D, respectively. Anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP) were from Cell Signaling. Alexa Fluor 488, 555, and 647 conjugated secondary antibodies were purchased from Thermoscientific.
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2

Immunodetection of Pluripotency Markers

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Mouse monoclonal antibody of β-actin was purchased from Santa Cruz. Mouse monoclonal antibodies to detect the pluripotent markers Lin28a, Nanog, Oct4, and Sox2 were purchased from Cell Signaling. Mouse and rabbit monoclonal antibodies of G3BP were purchased from BD Biosciences. Anti-alpha tubulin antibody (ab7291) and anti-beta-tubulin antibody were purchased from Abcam and Thermo Fischer Scientific, respectively. Anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP) were from Cell Signaling. Alexa Fluor 488, 555, and 647 conjugated secondary antibodies were purchased from Thermo Fisher Scientific.
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