Rabbit anti phospho p38 mapk thr180 tyr182

Manufactured by Cell Signaling Technology

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Rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) is an antibody product used for the detection of phosphorylated p38 MAPK at Thr180 and Tyr182 residues. This antibody is designed to specifically recognize the activated form of p38 MAPK.

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8 protocols using rabbit anti phospho p38 mapk thr180 tyr182

Western Blot Antibody Panel for Cell Signaling

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Rabbit anti-ERK1/2 MAPK, rabbit anti-phospho-ERK1/2 MAPK (Thr202/Tyr204), rabbit anti-p38 MAPK, rabbit anti-phospho-p38 MAPK (Thr180/Tyr182), rabbit anti-SAPK/JNK, rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185), rabbit anti-AKT, rabbit anti-phospho-AKT, rabbit anti-p65, rabbit anti-IkB, rabbit anti-MMP-2, rabbit anti-MMP-9, rabbit anti-lamin B1, rabbit anti- -β-catenin were from Cell Signaling Technology (USA). Rabbit anti-phospho-IkB was from Abcam (UK). Horsera-dish peroxidase-labeled goat anti-rabbit IgG, horsera-dish peroxidase-labeled goat anti-mouse IgG, mouse anti-GAPDH, and mouse anti-actin were from Multi-Sciences Biotech Co. Ltd (China). Rabbit anti-SLC8A2 was from MBL International Corporation (Japan).

Qu M., Yu J., Liu H., Ren Y., Ma C., Bu X, & Lan Q. (2017). The Candidate Tumor Suppressor Gene SLC8A2 Inhibits Invasion, Angiogenesis and Growth of Glioblastoma. Molecules and Cells, 40(10), 761-772.

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Western Blot Analysis of MAPK Signaling

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P815-HTR cells were lysed in radio-immunoprecipitation assay buffer (RIPA) buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EGTA, 1 mM EDTA, 1% Triton X-100, 1 mM Na₃VO₄, 5 mM NaF, and a protease inhibitor cocktail). A total of 30 µg of protein was separated per lane on 10% SDS-polyacrylamide gels, followed by transfer onto PVDF membranes (Millipore). The membranes were blocked in TBST (Tris-buffered saline containing 0.1% Tween-20) supplemented with 5% nonfat milk. Probing with the primary antibody was overnight at 4 °C; the blot was washed three times with TBST, and then incubated with appropriate secondary antibody (anti-rabbit HRP). After washing the blots three times in TBST, the protein bands were detected using a Western HRP substrate ECL kit (Luminata Forte, Millipore) and chemiluminescence imaging (Fusion FX, Vilber Lourmat). Primary antibodies were rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling #9211; 1: 1000), rabbit anti-p38 MAPK (Cell Signaling #9212; 1: 1000), rabbit anti-phospho-Erk1/2 (Thr202/Tyr204) (Cell signaling #9101; 1: 1000), and rabbit anti-Erk1/2 (Cell Signaling #9102; 1: 1000). Secondary antibody was goat anti-rabbit IgG HRP (Thermo Fisher; 1: 5000).

Park H., Lee C.W., Kang J., Sadra A, & Huh S.O. (2022). Glutamine increases stability of TPH1 mRNA via p38 mitogen-activated kinase in mouse mastocytoma cells. Molecular Biology Reports, 50(1), 267-277.

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Immunoblotting of Apoptosis-Related Proteins

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Immunoblotting was performed as in Hyvonen et al.^{54 (link)} Membranes were incubated overnight at +4 °C with rabbit anti-PDK1, rabbit anti-p38 MAPK, rabbit anti-phospho-p38 MAPK (Thr180/Tyr182), rabbit anti-phopho-Akt (Ser473), mouse anti-caspase-3 or rabbit anti-cleaved caspase-3 (Cell Signaling Technology), mouse anti-Pan Akt (R&D Systems, Minneapolis, MN, USA), rabbit anti-Bax or rabbit anti-Bcl2 (Abcam, Cambridge, UK), mouse anti-tubulin or mouse anti-actin IgGs (Sigma-Aldrich), followed by Alexa Fluor 680 (Invitrogen) or IRDye 800 (LI-COR, Lincoln, NE, USA) donkey anti-rabbit, anti-goat or anti-mouse IgGs. Detection and quantification was performed with an Odyssey Infrared Imager (LI-COR).

Saurus P., Kuusela S., Lehtonen E., Hyvönen M.E., Ristola M., Fogarty C.L., Tienari J., Lassenius M.I., Forsblom C., Lehto M., Saleem M.A., Groop P.H., Holthöfer H, & Lehtonen S. (2015). Podocyte apoptosis is prevented by blocking the Toll-like receptor pathway. Cell Death & Disease, 6(5), e1752-.

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Western Blot Analysis of Signaling Proteins

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Proteins obtained as described previously^{58 (link)} were blotted onto polyvinylidene fluoride membrane (Calbiochem/Merck Millipore) and incubated with following antibodies: rabbit anti-NTSR-1 (ANT-015), rabbit anti-NTSR-2 (ANT-016), Alomone Labs; rabbit anti-Bcl-2 (sc-783, Santa Cruz Biotechnology, Dallas, TX, USA); rabbit anti-Bcl-xL (#2764S), rabbit anti-phospho-Akt (Ser473) (#4060S), mouse anti-Akt (pan; #2920S), rabbit anti-phospho-Src family (Tyr416; #6943S), rabbit anti-Src (#2108S), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182; #9211S), rabbit anti-p38 MAPK (#8690S), rabbit anti-phospho-SAPK/JNK (Thr183/Tyr185; #4668S), and rabbit anti-SAPK/JNK (#9252), all from Cell Signaling Technology (Danvers, MA, USA); mouse anti-Giα1/2 antibody (06-236, Calbiochem/Merck Millipore); and anti-actin (A5441, Sigma-Aldrich). After washing (tris-buffered saline/0.1% Tween-20), the immunoreactions were detected by incubation for 2 h at RT with horseradish peroxidase-conjugated secondary Ab against mouse or rabbit Ig (P0447 and P0448, respectively, Agilent Technologies, Santa Clara, CA, USA), revealed with the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore). Western blot were detected using Bioimaging Systems (GeneSnap and GeneTool; Syngene, Cambridge, UK). Densitometric analyses were performed using the ImageJ software (NIH, Bethesda, MD, USA).

Abbaci A., Talbot H., Saada S., Gachard N., Abraham J., Jaccard A., Bordessoule D., Fauchais A.L., Naves T, & Jauberteau M.O. (2017). Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis. Oncogene, 37(6), 756-767.

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Western Blot Analysis of MAPK Signaling

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Protein content was quantified using the Bradford method. The samples were resolved by 10%–15% SDS-PAGE and transferred onto PVDF membranes (Millipore). After that, the membrane was blocked with 5% fat-free milk or BSA for 20–30 min and washed with TBST, followed by incubation with the indicated primary antibodies overnight at 4°C. The following primary antibodies were used: rabbit anti-JNK (Cell Signaling Technology, 9252), rabbit anti-phospho-JNK (Thr183/Tyr185) (Cell Signaling Technology, 4668), rabbit anti-p38 (Cell Signaling Technology, 2308), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology, 4511), rabbit anti-ATF2 (Cell Signaling Technology, 9226s), rabbit anti-phospho-ATF2 (Cell Signaling Technology, 5112), mouse anti-β-actin (Santa Cruz, SC-47778), rabbit anti-H2AX (Cell Signaling Technology, 7631), rabbit anti-phospho-Histone H2AX (Ser139) (Cell Signaling Technology, 3377s), and mouse anti-GST-tag (Proteintech Group, 66031-1-Ig). The chemiluminescence method (Bio-Rad) was used to detect the signals.

Xiao J., Lu H., Ma T., Ni X., Chang T., Liu M., Li N., Lu P., Ke C., Tian Q., Zou L., Wang F., Wang W., Zhang L., Yuan P., Liu L., Zhang J., Shi F., Duan Q, & Zhu F. (2022). Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2. Frontiers in Pharmacology, 13, 881042.

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Western Blot Analysis of Drosophila Proteins

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Flies were dissected into phosphate-buffered saline and abdomens were homogenized in standard lysis buffer. Equal amounts of protein were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto PVDF membrane (Thermo Scientific, Rockford, USA), blocked in 5% BSA, and incubated with 1:1000 rabbit β-Actin (Cell Signaling # 4967); 1:1000 rabbit anti-Phospho-Drosophila Akt (Ser505) (Cell Signaling #4054) and rabbit anti-Akt (Cell Signaling #9272); 1:1000 rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (Cell Signaling #9211) and rabbit anti-p38 MAPK (Cell Signaling #9212); 1:1000 rabbit anti-Phospho-p44/42 MAPK (Thr202/Tyr204) (Cell Signaling #4377) and rabbit anti-p44/42 MAPK (Cell Signaling #4695); and 1:1000 rabbit anti-Phospho-SAPK/JNK(Thr183/Tyr185) (Cell Signaling #4668) and rabbit anti-JNK (Santa Cruz Biotechnology, Inc. #sc-571). The primary antibodies were incubated overnight at 4°C. The secondary antibody was incubated with 1:5000 HRP-conjugated goat anti-rabbit or goat anti-mouse for 1 hour at room temperature and the signal developed using an ECL detection kit (Merk Millipore).

Eveland M., Brokamp G.A., Lue C.H., Harbison S.T., Leips J, & De Luca M. (2016). Knockdown expression of Syndecan in the fat body impacts nutrient metabolism and the organismal response to environmental stresses in Drosophila melanogaster. Biochemical and biophysical research communications, 477(1), 103-108.

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Comprehensive Antibody Panel for Cellular Analysis

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Goat anti-TIAR (C-18; sc-1749), goat anti-TIA1 (sc-1671), goat anti-eIF3B (eIF3eta; sc-16377), rabbit anti-p38 MAPK (sc-535), and mouse anti-G3BP (sc-81940) are from Santa Cruz; mouse anti-phospho-RPOL2A (Ser2; H5; ab24758), mouse anti-U1A (ab55751), rat anti-U1C (ab122901), rabbit anti-H3 (ab1791), rabbit anti-PRP19 (ab27692), rabbit anti-PRPF3 (ab187535), and rabbit anti-U2AF65 (ab37530) are from Abcam; rabbit anti-phospho-H3 (Ser10; #9701), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182; #9211), and rabbit anti-phospho-SF3B1 (Thr313; #25009) are from Cell Signaling Technology; mouse anti-U1-70K (05-1588) and mouse anti-puromycin (MABE343) are from Millipore; mouse anti-SC35 (S4045), rabbit anti-SON (HPA031755), and rabbit anti-CASC3 (HPA050262) are from Sigma-Aldrich; and rabbit anti-Calnexin polyclonal antibody (ADI-SPA-860-F) is from Enzo.

Sung H.M., Schott J., Boss P., Lehmann J.A., Hardt M.R., Lindner D., Messens J., Bogeski I., Ohler U, & Stoecklin G. (2023). Stress-induced nuclear speckle reorganization is linked to activation of immediate early gene splicing. The Journal of Cell Biology, 222(12), e202111151.

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RAGE Signaling Pathways in AGT Regulation

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A rabbit anti-RAGE antibody from Novus and a rabbit anti-AGT antibody from IBL were used in this study. A mouse anti-phospho p47phox (ser359) antibody and a rabbit anti-total p47phox antibody were purchased from Thermo Fisher Scientific. To elucidate RAGE signaling pathways involved in AGT regulation in PTC, mouse antiphospho-p42/44 MAPK (ERK 1/2, Thr202/Tyr204), a rabbit anti-p42/44 MAPK, mouse anti-phospho-STAT3 (Tyr705), rabbit anti-STAT3, rabbit anti-phospho-STAT1 (Tyr701), rabbit anti-STAT1, rabbit anti-phospho-p38 MAPK (Thr180/Tyr182), rabbit anti-p38 MAPK, rabbit anti-p65, rabbit anti-phospho-c-Jun (Ser73), rabbit anti-c-Jun, rabbit antiphospho-c-Fos (Ser32), and rabbit anti-c-Fos antibodies were purchased from Cell Signaling Technology and used in this study. In addition, rabbit anti-phospho-p65 (Ser536) antibody from Signalway Antibody was also used. Mouse anti-GAPDH antibody from Abcam was used as an internal control. IRDye-labeled anti-mouse IgG and anti-rabbit IgG antibodies were obtained from Li-Cor as secondary antibodies in Western blot analyses. Alexa Fluor 488 goat anti-rabbit IgG (H+L) antibody from Life Technologies was used as a secondary antibody in immunostaining.

Garagliano J.M., Katsurada A., Miyata K., Derbenev A.V., Zsombok A., Navar L.G, & Satou R. (2018). Advanced Glycation End Products Stimulate Angiotensinogen Production in Renal Proximal Tubular Cells. The American journal of the medical sciences, 357(1), 57-66.

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