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Fluorinert fc 43

Manufactured by 3M
Sourced in France

Fluorinert FC-43 is a fully fluorinated, thermally and chemically stable liquid produced by 3M. It is designed for use as a coolant, heat transfer medium, and dielectric fluid in specialized laboratory equipment and applications.

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5 protocols using fluorinert fc 43

1

Wheat Infection by Powdery Mildew

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Twelve-day-old wheat plantlets were inoculated with 5 mL of a suspension of Bgt spores (500 000 spores/mL in Fluorinert FC43, heptacosafluorotributhylamine 3M, Cergy-Pontoise, France) with a sprayer (ITW Surfaces et Finitions, Valence, France).
Mock-infected plants were sprayed with 5 mL of Fluorinert FC43 (heptacosafluorotributhylamine 3M, Cergy-Pontoise, France). Twelve days after inoculation, the symptoms were checked through the development of white colonies on the first leaf, what confirms the successful infection of plantlets by Bgt.
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2

Membrane Pore Size Characterization

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The membrane pore size distribution was characterized
by using a Porolux 500 capillary flow porometer (IB-FT GmbH, Berlin,
Germany) for the symmetric porous membranes. Before each measurement,
the membranes (ϕ 25 mm) were immersed for 15 min in perfluorotributylamine
(Fluorinert FC-43, 3M Belgium). Measurements were performed from 0.1
to 6.0 bar of applied nitrogen pressure. Reported values for mean
pore size are the average of at least two measurements with the standard
deviation of the repeats used for the error bars.
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3

Microfluidic PLGA Particle Synthesis

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Poly(d,l lactic-co-glycolic acid) (PLGA, 38,000–54,000, 50:50 lactic acid: glycolic acid, acid terminated; SIGMA); Polyvinyl alcohol (PVA, average 30,000–70,000 mw, 87–90% hydrolyzed, Sigma Aldrich); High-speed FASTCAM camera (PCI-10K, Photron LTd.); high-speed Phantom monochrome camera (V310, Vision Research); Inverted microscope (Nikon); Plastic 1 ml Luer Norm-Ject syringes (Henke Sass Wolf); Glass 250 µl–1 ml Hamilton gastight instrument syringes (Hamilton); Pico Plus Syringe pumps (Harvard Apparatus); Teflon (PTFE) tubing (wall thickness 0.012, AWG 20, ICO Rally); PDMS (poly(dimethyl) siloxane, Sylgard 184, Dow Corning), Teflon AF (DuPont); Fluorinert FC-43 (3M); Biopsy punches (Integra Miltex); Air plasma chamber (Harrick Scientific); Dimethyl Carbonate (DMC, anhydrous 99%, SIGMA), PBS (Phosphate Buffered Saline, pH 7.4, without Calcium or Magnesium, Lonza).
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4

Porous Rock Core Flow Characterization

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Brown"; Kocurek Industries Inc.), of diameter and length 38.1 mm (1.5 in) and 6 cm, respectively, was used. The total porosity, determined gravimetrically by imbibition of deionised water by vacuum saturation was 37.6 ± 0.4%. The core was then placed in a cylindrical Ergotech PEEK rock core flow cell and a confining pressure of 2 MPa was applied using 3M Fluorinert FC-43, which is NMR silent in typical 1 H chemical shift ranges. The cell was contained in a closed-loop system with a backpressure regulator, and the confining pressure was maintained using a Gilson model 307 pump. A constant flow rate of water of 10 mL min -1 was imposed using a Quizix QX-1500HC dual-cylinder syringe pump.
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5

Heterogeneous Carbonate Imbibition Experiment

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A cylindrical plug from a vuggy, heterogeneous carbonate outcrop rock of 38.0 mm diameter and 59.7 mm length and an overall porosity of 19.1% was imbibed with deionised water through vacuum saturation. The core was then placed in a cylindrical Ergotech PEEK rock core flow cell. A confining pressure was applied using 3M Fluorinert FC-43, which is NMR silent in typical 1 H chemical shift ranges. The flow cell was connected into a closed flow loop, and a constant flow rate of water of 10 mL min -1 was imposed using a Quizix QX-1500 HC dual-cylinder syringe pump.
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