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High performance liquid chromatography

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High-performance liquid chromatography (HPLC) is a laboratory analytical technique used to separate, identify, and quantify components in a mixture. HPLC employs a liquid mobile phase to separate analytes, which are then detected by a variety of detectors. The core function of HPLC is to provide efficient and precise separation and analysis of complex samples.

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Lab products found in correlation

Hplc grade methanol, by Fujifilm (3 mentions) Urease, by Merck Group (1 mentions) Chloroform, by Fujifilm (1 mentions) Cytarabine, by Tokyo Chemical Industry (1 mentions) Decitabine, by Fujifilm (1 mentions) Mycophenolate mofetil, by Merck Group (1 mentions) Ammonium hydroxide solution, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions) Acetonitrile, by Fujifilm (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Ammonium bicarbonate, by Merck Group (1 mentions) Trigonelline, by LGC (1 mentions) Sodium 1 octanesulfonate, by Tokyo Chemical Industry (1 mentions) N n diisopropylethylamine dipea, by Tokyo Chemical Industry (1 mentions) Hexane, by Fujifilm (1 mentions) Analytical grade ethanol, by Kanto Chemical (1 mentions) Fucoxanthin standard, by Fujifilm (1 mentions) 2 propanol, by Fujifilm (1 mentions)

5 protocols using high performance liquid chromatography

1

Metabolite Derivatization and Analysis

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N-methyl-N-(trimethylsilyl)-trifluoroacetamide with 1% trimethylchlorosilane (MSTFA + 1% TMCS) was purchased from Thermo Scientific (Pittsburgh, PA, USA). O-methylhydroxylammonium chloride (methoxylamine hydrochloride), pyridine, 2,2-dimethyl succinic acid, myristic acid d27, pesticide-analytical-grade chloroform and hexane, and high-performance liquid chromatography (HPLC)-analytical-grade methanol were purchased from Wako Pure Chemical (Tokyo, Japan). Urease was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Toda K., Kokushi E., Uno S., Shiiba A., Hasunuma H., Fushimi Y., Wijayagunawardane M.P., Zhang C., Yamato O., Taniguchi M., Fink-Gremmels J, & Takagi M. (2017). Gas Chromatography-Mass Spectrometry for Metabolite Profiling of Japanese Black Cattle Naturally Contaminated with Zearalenone and Sterigmatocystin. Toxins, 9(10), 294.
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2

Analysis of Antimetabolite Drug Compounds

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Mycophenolic acid, azacitidine, decitabine, 5‐FU, IMP disodium salt, GTP, high‐performance liquid chromatography (HPLC) grade methanol, acetonitrile (ACN), dimethyl sulfoxide (DMSO), and chloroform were purchased from Wako Pure Chemical Industries (Osaka, Japan). 5‐Azacytidine 5′‐triphosphate (aza‐CTP) ammonium salt was purchased from American Biochemicals (San Diego, CA, USA). Cytarabine and 2′‐deoxy‐2′‐fluorocytidine hydrate (2D2F) were purchased from Tokyo Chemical Industry (Tokyo, Japan). FF‐10501‐01 (3/4 hydrate of FF‐10501) and FF‐10501RMP disodium salt were provided by Toyama Chemical (Tokyo, Japan). Gemcitabine hydrochloride was purchased from Teva Pharmaceutical Industries (Netanya, Israel). Mycophenolate mofetil, ammonium bicarbonate, and ammonium hydroxide solution were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Phosphate‐buffered saline (PBS) was purchased from Life Technologies (Gaithersburg, MD, USA).
Murase M., Iwamura H., Komatsu K., Saito M., Maekawa T., Nakamura T., Yokokawa T, & Shimada Y. (2016). Lack of cross‐resistance to FF‐10501, an inhibitor of inosine‐5′‐monophosphate dehydrogenase, in azacitidine‐resistant cell lines selected from SKM‐1 and MOLM‐13 leukemia cell lines. Pharmacology Research & Perspectives, 4(1), e00206.
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3

Supercritical CO2 Decaffeination of Arabica Coffee

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Arabica coffee (Coffea arabica L.) beans with and without decaffeination were procured from Super Critical Technology Centre Co. Ltd. (Mie, Japan) . Coffee bean decaffeination was performed via pilot-scale (30 L) supercritical CO 2 extraction with water (co-solvent) , as described previously 29, 30) . High-performance liquid chromatography (HPLC) -grade methanol was purchased from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan) . Caffeine, caffeic acid, chlorogenic acid, nicotinic acid, theophylline, and phosphoric acid were purchased from Wako Pure Chemical Corp. (Osaka, Japan) . Cryptochlorogenic acid and trigonelline were procured from Toronto Research Chemicals (Toronto, ON, Canada) . Neochlorogenic acid and sodium 1-octanesulfonate were procured from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan) .
Honda M., Takezaki D., Tanaka M., Fukaya M, & Goto M. (2022). Effect of Roasting Degree on Major Coffee Compounds: A Comparative Study between Coffee Beans with and without Supercritical CO2 Decaffeination Treatment. Journal of oleo science, 71(10).
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4

Tomato Powder Characterization and Analysis

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Tomato powder (Kagome Tomato Powder; lycopene content＝241.9 mg/100 g, total Z-isomer ratio of lycopene ＝5.3％, moisture content＝6.9％, average particle size＝ 275.9 μm) was purchased from Kagome Co., Ltd. (Tokyo, Japan) ( Fig. S1) . This tomato powder was produced by drying and grinding Italian tomato paste. Analytical-grade acetone and ethanol were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan) , and high-performance liquid chromatography (HPLC) -grade hexane was purchased from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan) . N,N-Diisopropylethylamine (DIPEA) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) . Carbon dioxide (＞99.5％ purity) was obtained from Daiwa Corp. (Nagoya, Japan) .
Murakami K., Goto M, & Honda M. (2022). High-temperature Supercritical CO2 Extraction of Lycopene from Tomato Powder for Enhancing Z-Isomerization and Recovery of Lycopene. Journal of oleo science, 71(9).
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5

Extraction and Analysis of Fucoxanthin from Undaria pinnatifida

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Dried Undaria pinnatifida (moisture content: 5.5％) was purchased from a local supermarket in Nagoya, Japan, and homogenized using a food processor (SJM-180G; Siroca, Inc., Tokyo, Japan) . A fucoxanthin standard was purchased from Wako Pure Chemical Corp. (Osaka, Japan) . Analytical-grade ethanol and acetone were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan) , and high-performance liquid chromatography (HPLC) -grade methanol, 2-propanol, and hexane were purchased from Fujifilm Wako Pure Chemical Corp. (Osaka, Japan) . Carbon dioxide (＞99.5％ purity) was obtained from Daiwa Corp. (Nagoya, Japan) .
Honda M., Murakami K., Takasu S, & Goto M. (2022). Extraction of Fucoxanthin Isomers from the Edible Brown Seaweed Undaria pinnatifida Using Supercritical CO2: Effects of Extraction Conditions on Isomerization and Recovery of Fucoxanthin. Journal of oleo science, 71(8).
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