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Alexa fluor 568 conjugated goat anti rabbit secondary

Manufactured by Thermo Fisher Scientific
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Alexa Fluor 568-conjugated goat anti-rabbit secondary is a fluorescently labeled antibody that can be used to detect and visualize rabbit primary antibodies in various biological applications. It consists of a goat-derived secondary antibody that specifically binds to rabbit immunoglobulins, conjugated with the Alexa Fluor 568 fluorescent dye.

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Lab products found in correlation

Ab27957, by Santa Cruz Biotechnology (2 mentions) Fl 197, by Santa Cruz Biotechnology (2 mentions) Facscanto, by BD (2 mentions) Facscalibur, by BD (2 mentions) Dnase 1, by Roche (2 mentions) Dispase, by Roche (2 mentions) Collagenase a, by Roche (2 mentions)

Close spelling variants of this product

Alexa Fluor® 568 conjugated goat anti-rabbit IgG secondary antibody Alexa Fluor 568-conjugated goat anti-rabbit IgG (H + L) secondary antibodies Alexa Fluor 568-conjugated goat anti-rabbit secondary antibody Alexa Fluor 568-conjugated goat anti-rabbit Alexa Fluor 568 goat anti-rabbit Alexa 568-conjugated goat anti-rabbit Anti-goat Alexa-Fluor 568 Alexa Fluor 568 anti-rabbit Alexa 568 goat anti-rabbit Alexa Fluor 568 secondary

2 protocols using alexa fluor 568 conjugated goat anti rabbit secondary

1

Isolation and Flow Cytometric Analysis of Lung and Liver Cells

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Bone marrow was prepared for flow cytometry as previously described1 (link). For analysis of lung, tissues were minced and then digested at 37°C for 20 min with an enzyme cocktail (collagenase A, dispase and DNaseI, Roche Applied Science). Single-cell suspensions were prepared by filtering through a 70-μm strainer and passing through an 18G syringe. Lung fibroblasts were identified by flow cytometry using an anti-mouse rabbit polyclonal S100A4 (1:50, Abcam; ab27957), or SPC (1:100, Santa Cruz; FL-197), revealed by Alexa Fluor 568-conjugated goat anti-rabbit secondary (A-11011, Life Technologies, 1:400). For liver, tissues were mechanically dissociated, and single-cell suspensions were filtered through a 40-μm strainer. Allophycocyanin-conjugated F4/80 (1:100, eBioscience; clone BM8) was used to identify liver macrophages by flow cytometry. Cell fluorescence indicating fluorescently labelled exosome uptake was analysed using a FACSCalibur or a FACSCanto (Beckton Dickinson). FACS data was analysed with FlowJo software (TreeStar Inc.).
Hoshino A., Costa-Silva B., Shen T.L., Rodrigues G., Hashimoto A., Mark M.T., Molina H., Kohsaka S., Di Giannatale A., Ceder S., Singh S., Williams C., Soplop N., Uryu K., Pharmer L., King T., Bojmar L., Davies A.E., Ararso Y., Zhang T., Zhang H., Hernandez J., Weiss J.M., Dumont-Cole V.D., Kramer K., Wexler L.H., Narendran A., Schwartz G.K., Healey J.H., Sandstrom P., Labori K.J., Kure E.H., Grandgenett P.M., Hollingsworth M.A., de Sousa M., Kaur S., Jain M., Mallya K., Batra S.K., Jarnagin W.R., Brady M.S., Fodstad O., Muller V., Pantel K., Minn A.J., Bissell M.J., Garcia B.A., Kang Y., Rajasekhar V.K., Ghajar C.M., Matei I., Peinado H., Bromberg J, & Lyden D. (2015). Tumour exosome integrins determine organotropic metastasis. Nature, 527(7578), 329-335.
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2

Isolation and Flow Cytometric Analysis of Lung and Liver Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow was prepared for flow cytometry as previously described1 (link). For analysis of lung, tissues were minced and then digested at 37°C for 20 min with an enzyme cocktail (collagenase A, dispase and DNaseI, Roche Applied Science). Single-cell suspensions were prepared by filtering through a 70-μm strainer and passing through an 18G syringe. Lung fibroblasts were identified by flow cytometry using an anti-mouse rabbit polyclonal S100A4 (1:50, Abcam; ab27957), or SPC (1:100, Santa Cruz; FL-197), revealed by Alexa Fluor 568-conjugated goat anti-rabbit secondary (A-11011, Life Technologies, 1:400). For liver, tissues were mechanically dissociated, and single-cell suspensions were filtered through a 40-μm strainer. Allophycocyanin-conjugated F4/80 (1:100, eBioscience; clone BM8) was used to identify liver macrophages by flow cytometry. Cell fluorescence indicating fluorescently labelled exosome uptake was analysed using a FACSCalibur or a FACSCanto (Beckton Dickinson). FACS data was analysed with FlowJo software (TreeStar Inc.).
Hoshino A., Costa-Silva B., Shen T.L., Rodrigues G., Hashimoto A., Mark M.T., Molina H., Kohsaka S., Di Giannatale A., Ceder S., Singh S., Williams C., Soplop N., Uryu K., Pharmer L., King T., Bojmar L., Davies A.E., Ararso Y., Zhang T., Zhang H., Hernandez J., Weiss J.M., Dumont-Cole V.D., Kramer K., Wexler L.H., Narendran A., Schwartz G.K., Healey J.H., Sandstrom P., Labori K.J., Kure E.H., Grandgenett P.M., Hollingsworth M.A., de Sousa M., Kaur S., Jain M., Mallya K., Batra S.K., Jarnagin W.R., Brady M.S., Fodstad O., Muller V., Pantel K., Minn A.J., Bissell M.J., Garcia B.A., Kang Y., Rajasekhar V.K., Ghajar C.M., Matei I., Peinado H., Bromberg J, & Lyden D. (2015). Tumour exosome integrins determine organotropic metastasis. Nature, 527(7578), 329-335.
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