Dehydroepiandrosterone dhea

Testosterone, androsterone, β-estradiol, α-estradiol, epiandrosterone, cortisone, corticosterone, 11-deoxycortisol, lithium acetate, sodium acetate, potassium acetate, and water were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aldosterone, dehydroepiandrosterone (DHEA), and methanol were purchased from Fisher Scientific (Pittsburg, PA, USA). Steroid solutions contained 25 μM of each steroid for the specified mixtures with 100–150 μM of lithium acetate, sodium acetate, or potassium acetate. The complex mixture contained 10 μM of all steroids and 100 μM of lithium acetate and 100 μM of potassium acetate.

Testosterone, androsterone, epiandrosterone, cortisone, corticosterone, hydrocortisone, 11-deoxycortisol, corticosterone-d4, lithium acetate, sodium acetate, potassium acetate, and water were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aldosterone, dehydroepiandrosterone (DHEA), and methanol were purchased from Fisher Scientific (Pittsburg, PA, USA). The steroid mixture was made containing all steroids at a concentration of 500 nM in 50% HPLC grade water/methanol (the names, molecular weights, and structures of the steroids studied here are provided in Table S1 of the Supplementary Material). For analysis, 2.5 μL were injected into the LC (approximating 375 pg of each component on column). Water and methanol with lithium acetate added at a concentration of 150 μM (to form lithium ion adducts) were used as the mobile phase.