Taqman snp genotyping assays probe

All mice used were housed in a 12 hr light-dark cycle. For SLC48A1 pups, genetic segregation was computed on 21 day old (P21) mice pups. SLC48A1 mice were genotyped from tail genomic DNA extracts using a custom ordered TaqMan SNP Genotyping Assays probe (ThermoFisher Scientific) on a Bio-rad CFX Connect system. For HMOX1/SLC48A1 mice, genetic segregation was computed on 5 day old (P6) mice pups via toe-clip DNA extracts. HMOX1 was genotyped by PCR using primers HMOX1 KO Forward 5’-GCTTGGGTGGAGAGGCTATTC-3’, HMOX1 KO Reverse 5’-CAAGGTGAGATGACAGGAGATC-3’, HMOX1 WT Forward 5’-GTACACTGACTGTGGGTGGGGGAG-3’, HMOX1 WT Reverse 5’-AGGGCCGAGTAGATATGGTAC-3’. Mice in all studies were males unless otherwise noted, although initial experiments to exclude gender variation were done using both males and females. All animal protocols were approved by the Institutional Animal Care and Use Committee at the University of Maryland, College Park (IACUC Animal Study Protocol R-NOV-18–61).

All mice used were housed in a 12 hr light-dark cycle. For SLC48A1 pups, genetic segregation was computed on 21 day old (P21) mice pups. SLC48A1 mice were genotyped from tail genomic DNA extracts using a custom ordered TaqMan SNP Genotyping Assays probe (ThermoFisher Scientific) on a Bio-rad CFX Connect system. For HMOX1/SLC48A1 mice, genetic segregation was computed on 5 day old (P6) mice pups via toe-clip DNA extracts. HMOX1 was genotyped by PCR using primers HMOX1 KO Forward 5’-GCTTGGGTGGAGAGGCTATTC-3’, HMOX1 KO Reverse 5’-CAAGGTGAGATGACAGGAGATC-3’, HMOX1 WT Forward 5’-GTACACTGACTGTGGGTGGGGGAG-3’, HMOX1 WT Reverse 5’-AGGGCCGAGTAGATATGGTAC-3’. Mice in all studies were males unless otherwise noted, although initial experiments to exclude gender variation were done using both males and females. All animal protocols were approved by the Institutional Animal Care and Use Committee at the University of Maryland, College Park (IACUC Animal Study Protocol R-NOV-18–61).