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4 hne antibody ab46545

Manufactured by Abcam
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The 4-HNE antibody (ab46545) is a lab reagent used to detect the presence of 4-hydroxynonenal (4-HNE), a biomarker of oxidative stress. This antibody is intended for research use only.

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Lab products found in correlation

Anti acsl4, by Cell Signaling Technology (1 mentions) K48 ubiquitin antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

4-HNE (ab46545) 4-HNE antibody 4-HNE Ab46545

2 protocols using 4 hne antibody ab46545

1

Ferroptosis-Inhibition Compound Evaluation

Cited 1 time
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Ferrostatin-1 (347174-05-4), liproxstatin-1 (950455-15-9), trolox (53188-07-1), deferoxamine (138-14-7), RSL-3 (1219810-16-8), erastin (571203-78-6), sulfasalazine (599-79-1), atorvastatin (599-79-1), KU60019 (925701-46-8) were purchased from Cayman. The concentration used is shown in Supplementary Table. S1. C11BODIPY (D3861) was purchased from Invitrogen. Mouse recombinant IFNγ (485-MI-100), anti-mouse IFNγ block antibody (MAB485), human recombinant IFNγ (285-IF-100) were purchased from R&D. Anti-mouse IFNGR1 antibody (16-1193-85) was purchased from Thermo Fisher. The following antibody were used for immunoblots: anti-human SLC7A11 antibody (12691), anti-β-actin (12262), anti-GAPDH (2118), anti-STAT1 (9172), anti-phospho-STAT1 (9167), anti-ATM (2873) and anti-ACSL4 (4047) were bought from Cell Signaling Technology. Anti-GPX4 (ab41787) was from Abcam. Additionally, IgG (BE0087, BE0090), anti-mouse CTLA4 (BE0131), anti-mouse PD-L1 (BE0101) and anti-mouse CD8 (BE0117) were purchased from BioXcell. 4-HNE antibody (ab46545) was purchased from Abcam. Cyst(e)inase was generated as previously described (18 (link)).
Lang X., Green M.D., Wang W., Yu J., Choi J.E., Jiang L., Liao P., Zhou J., Zhang Q., Dow A., Saripalli A.L., Kryczek I., Wei S., Szeliga W., Vatan L., Stone E.M., Georgiou G., Cieslik M., Wahl D.R., Morgan M.A., Chinnaiyan A.M., Lawrence T.S, & Zou W. (2019). Radiotherapy and immunotherapy promote tumoral lipid oxidation and ferroptosis via synergistic repression of SLC7A11. Cancer discovery, 9(12), 1673-1685.
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2

Necroptosis Signaling Pathway Antibodies

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Receptor-interacting serine/threonine-protein kinase 1 antibody (3493; 1:1,000), RIP3 antibody (15828; 1:1,000), phosphor-RIP1 antibody (p-RIP1; 1:1,000), Ser166 (31122; 1:1,000), CaMKII antibody (3362; 1:1,000), K48-ubiquitin antibody (8081; 1:1,000), and secondary antibodies (1:10,000) were bought from Cell Signaling Technology (Danvers, MA, United States). Phosphor-RIP3 antibody (p-RIP3) (ab195117; 1:1,000), 4-HNE antibody (ab46545; 1:1,000), phosphor-MLKL antibody (p-MLKL) (ab196436; 1:1,000), phosphor-CaMKII antibody (p-CaMKII) (ab32678; 1:1,000) were purchased from Abcam (Cambridge, MA, United States). β-Actin antibody (6008-1-Ig; 1:5,000) and MLKL antibody (66675; 1:1,000) were bought from Proteintech (Rosemont, IL, United States). Cycloheximide (CHX, 25 μg/ml) (5087390001) was bought from Sigma-Aldrich (Steinheim, Germany).
Zhai X., Wang W., Sun S., Han Y., Li J., Cao S., Li R., Xu T., Yuan Q., Wang J., Wei S, & Chen Y. (2021). 4-Hydroxy-2-Nonenal Promotes Cardiomyocyte Necroptosis via Stabilizing Receptor-Interacting Serine/Threonine-Protein Kinase 1. Frontiers in Cell and Developmental Biology, 9, 721795.
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