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Oligo probe

Manufactured by Merck Group
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The Oligo probe is a laboratory instrument designed for the detection and analysis of specific DNA or RNA sequences. It utilizes synthetic oligonucleotides, known as probes, to target and bind to complementary sequences within a sample. The core function of the Oligo probe is to facilitate the identification and characterization of genetic material.

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Lab products found in correlation

C 1 magnetic beads, by Thermo Fisher Scientific (2 mentions) Trizol reagent, by Merck Group (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

2 protocols using oligo probe

1

Quantifying circUGGT2 and miR-526-5p

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The biotinylated-circUGGT2 probe (circUGGT2 probe) and Oligo probe were obtained from Sigma. In brief, the circUGGT2 probe was incubated with C-1 magnetic beads (Life Technologies) to generate the probe-coated beads. Thereafter, the lysates of HCC cells were incubated with the probe-coated beads. The bound RNA was extracted with TRIzol reagent (Sigma). The levels of circUGGT2 and miR-526-5p were detected with qRT-PCR.
Kong Q., Fan Q., Ma X., Li J, & Ma R. (2020). CircRNA circUGGT2 Contributes to Hepatocellular Carcinoma Development via Regulation of the miR-526b-5p/RAB1A Axis. Cancer Management and Research, 12, 10229-10241.
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2

Profiling CircRNA-miRNA Interactions

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In short, the hsa_circ_0000735 probe or Oligo probe (Sigma) was incubated with C-1 magnetic beads (Life Technologies) to generate the probe-coated beads. Thereafter, the probe-coated beads were incubated with sonicated PC-3/DTX and DU145/DTX cells. The RNA complexes were extracted with the RNeasy Mini Kit (Qiagen). The levels of miR-1182, miR-331-3p, miR-583, and miR-7 were evaluated with qRT-PCR.
Gao Y., Liu J., Huan J, & Che F. (2020). Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7. Cancer Cell International, 20, 334.
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